Standard and nested PCR for MDV-2
Evaluation of nested PCR in diagnosis of fungal rhinosinusitis
... Rhinosinusitis is a clinical entity produced by several kinds of organisms, and the management of each type requires identification of the etiologic agent through direct smear and culture, which are valuable in the ...
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Rapid Multiplex Nested PCR for Detection of Respiratory Viruses
... (fast PCR machine; Applied Biosystems, Foster City, CA) was ...fast PCR master mix (GeneAmp; Applied Biosystems, Foster City, CA), a 35-cycle PCR assay could be completed within 35 min, compared to ...
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Comparison of two nested PCR methods for the detection of human malaria
... by nested PCR. The sensitivity and specificity of nested PCR assay using the two primers were calculated with reference to microscopy as the ‘gold ...
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Detection of Sporothrix schenckii in Clinical Samples by a Nested PCR Assay
... MATERIALS AND METHODS Microorganisms. Five clinical isolates of S. schenckii obtained from the De- partment of Clinical Microbiology of Chang Gung Memorial Hospital and one ATCC 10213 strain of S. schenckii were used and ...
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A highly sensitive modified nested PCR to enhance case detection in leishmaniasis
... Validation and data analysis A sample was taken as Leishmania positive if it was positive by any one of the control tests performed viz. LM, IVC or CPCR. Reproducibility or repeatability of the assay was determined over ...
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Analysis of malaria diagnosis discrepancies between RDTs and microscopy by nested PCR
... The PCR assay de- tected submicroscopic infection in almost 23% of the microscopy-negative samples, whereas plasmodial DNA was not found in 77% of the Acon ® HRP2 positive- microscopy negative ...of PCR ...
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The Diagnostic Utility of Bact/ALERT and Nested PCR in the Diagnosis of Tuberculous Meningitis
... the nested PCR, except for the primers which were ...specific nested PCR product size was 219 ...with standard molecular weight ...
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Genetic Diversity Block 2 of Surface Protein-1 in Plasmodium Falciparum Merozoite by Nested-PCR Method in Southeastern Iran
... TLA PCR Premix (Bioneer, Korea) which included the following ingredients: the enzyme TLA DNA polymerase (1 U), Deoxynucleotide Triphosphate (250 μM), buffer 10X (2μl), stabilizer and dye ...stage PCR ...
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Sensitivity and Specificity of Nested PCR for Diagnosing Malaria: Cases in Several Areas of Indonesia
... gold standard to diagnose malaria ...of nested PCR compared to microscopic examination in diagnosing malaria ...nest PCR was done in Laboratory of Parasitology and Laboratory of Central ...
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Monitoring of leukocyte cytomegalovirus DNA in bone marrow transplant recipients by nested PCR
... a nested PCR assay that circumvented the need for DNA probing was used for weekly monitoring of 101 BMT ...leukocyte PCR assays under standard conditions defeats the purpose of the ...
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Colorimetric one tube nested PCR for detection of Trichomonas vaginalis in vaginal discharge
... one-tube nested PCR was developed for the detection of Trichomonas vaginalis in clinical vaginal discharge ...to nested PCR for semiquan- titative determination of ...parasites. PCR of ...
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ECKER LAB PROTOCOL. Nested PCR and Sequencing of T-DNA Junctions in Arabidopsis
... Note: This method sometimes fails to work when a new stock is tested. This may be a result of incorrect DNA concentration, impure primers, etc. Always have some good primer stock stored so that new stocks can be compared ...
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Detection of Human Papillomavirus in Squamous Cell Carcinoma of the Lung by Semi-nested PCR
... DNA was extracted from four to five 10 µm tissue sections of paraffin blocks by standard phenol- chloroform procedure after treatment with proteinase K. To avoid carry-over of samples the microtome blade was ...
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Identification of Genetic Evidence for Dobrava Virus Spillover in Rodents by Nested Reverse Transcription (RT) PCR and TaqMan RT PCR
... RNA standard transcribed from a DOBV S segment ligated into pCRII (Invitrogen, Breda, The ...10 2 ; correlation coefficient, ...the nested PCR with the synthetic RNA standard and found ...
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Application of a nested, multiplex PCR to psittacosis outbreaks
... a nested, multiplex PCR for simultaneous detection of three species of chlamydiae in human and avian ...The PCR was designed to increase sensitivity and to circumvent inhibitors of PCR present ...
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Nested PCR Assay for Detection of Granulocytic Ehrlichiae
... the PCR amplification was assessed by using a plasmid control and was shown to be fewer than 2 copies of the 16S rRNA ...single-step PCR assays were negative for each of the seven human serum samples ...
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Evaluation of AMPLICOR Neisseria gonorrhoeae PCR Using cppB Nested PCR and 16S rRNA PCR
... gonorrhoeae PCR (Roche Diagnostic Systems, Branchburg, ...three PCR tests were assessed with 3 geographically diverse ...in-house nested cppB gene and the 16S rRNA PCR methods were greater ...
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Detection and strain identification of Actinobacillus actinomycetemcomitans by nested PCR
... Two-step PCR amplification directly from plaque samples combined with sequence analysis of the ribosomal DNA spacer region provides a sensitive assay for detection and strain identificat[r] ...
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Validation of nested Bordetella PCR in pertussis vaccine trial
... MATERIALS AND METHODS Clinical material and case definition. A total of 9,829 children born during 1992 were enrolled in a phase 3 pertussis vaccine efficacy trial which took place from 1992 to 1995 in Sweden, receiving ...
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Broadly Reactive Nested Reverse Transcription PCR Using an Internal RNA Standard Control for Detection of Noroviruses in Stool Samples
... RT- PCR and Southern hybridization. In some cases, the sensitivity of nRT-PCR appeared to be 10-fold higher or ...or 2-log- higher positive dilution for GII norovirus strains, which supports the ...
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