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Stem cell tracking and MRI

Magnetic reporter genes for MRI-based stem cell tracking

Magnetic reporter genes for MRI-based stem cell tracking

... normal cell functions and cells can recover up to normal levels in a few ...parental cell line, differentiation potential was assessed at P8 post-transduction, again after FACS ...

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Superparamagnetic Iron Oxide Nanoparticles as MRI contrast agents for Non-invasive Stem Cell Labeling and Tracking

Superparamagnetic Iron Oxide Nanoparticles as MRI contrast agents for Non-invasive Stem Cell Labeling and Tracking

... in stem cell labeling due to their high MR sensitivity and amenability to surface ...and MRI tech- nologies for stem cell biology, several problems re- main to be solved: 1) How to ...

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Design considerations for the synthesis of polymer coated iron oxide nanoparticles for stem cell labelling and tracking using MRI

Design considerations for the synthesis of polymer coated iron oxide nanoparticles for stem cell labelling and tracking using MRI

... particular stem cell line, although uptake values can be subject of large errors depending on the labelling procedure and quantification method ...label stem cells, they were designed as ...

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Design considerations for the synthesis of polymer coated iron oxide nanoparticles for stem cell labelling and tracking using MRI

Design considerations for the synthesis of polymer coated iron oxide nanoparticles for stem cell labelling and tracking using MRI

... particular stem cell line, although uptake values can be subject of large errors depending on the labelling procedure and quantification method ...label stem cells, they were designed as ...

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Nanoparticles Based Stem Cell Tracking in Regenerative Medicine

Nanoparticles Based Stem Cell Tracking in Regenerative Medicine

... in tracking stem cells by MRI both in vitro and in vivo but are not limited to just stem cells, they can also be ap- plied in other tracking ...an MRI scan, allowing the infected ...

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Stem cell tracking using iron oxide nanoparticles

Stem cell tracking using iron oxide nanoparticles

... neural stem cells where the concentration of SPIONs was shown to decrease by 50% every 3 days in ...labeled stem cells, the density of the cells is considerably reduced over time, again leading to a ...

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Bright Polymer Dots Tracking Stem Cell Engraftment and Migration to Injured Mouse Liver

Bright Polymer Dots Tracking Stem Cell Engraftment and Migration to Injured Mouse Liver

... Current cell tracking techniques mainly include positron emission tomography (PET) [12], magnetic resonance imaging (MRI) [13, 14], and fluorescence imaging [15, ...for cell tracking ...

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MRI tracking of Stem Cells in Multiple Sclerosis

MRI tracking of Stem Cells in Multiple Sclerosis

... of cell labeling and tracking are very ...cells, MRI of labeled cells in vitro, assessment of cell viability, animal model, preparation of cells for transplantation, MRI of transplanted ...

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Commercial Nanoparticles for Stem Cell Labeling and Tracking

Commercial Nanoparticles for Stem Cell Labeling and Tracking

... imaging, MRI and photoacoustic imaging) for labeling and tracking of stem ...as MRI contrast agents have the longest history of com- mercialization and use in cell labeling and in vivo ...

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Labeling of mesenchymal stem cells for MRI with single cell sensitivity

Labeling of mesenchymal stem cells for MRI with single cell sensitivity

... for MRI cell ...for MRI, 26 and cell labeling has been achieved after cell incubation with nanocomplexes of ferumoxy- ...sensitive MRI detection has been accomplished for 1,000 ...

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Labeling of mesenchymal stem cells for MRI with single-cell sensitivity

Labeling of mesenchymal stem cells for MRI with single-cell sensitivity

... for MRI cell ...for MRI, 26 and cell labeling has been achieved after cell incubation with nanocomplexes of ferumoxy- ...sensitive MRI detection has been accomplished for 1,000 ...

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The Effectiveness of Ferritin as a Contrast Agent for Cell Tracking MRI in Mouse Cancer Models

The Effectiveness of Ferritin as a Contrast Agent for Cell Tracking MRI in Mouse Cancer Models

... macrophages, stem cells are known to have tumor tropism, as well as wound-relat- ed ...mal stem cells may be useful as a gene delivery tool in cancer ...a tracking system to monitor targeting ...

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A safe and effective magnetic labeling protocol for MRI based tracking of human adult neural stem cells

A safe and effective magnetic labeling protocol for MRI based tracking of human adult neural stem cells

... neural stem cells and therefore can store much higher amounts of VSOP, while macrophages are particularly efficient in uptaking ...higher cell concentrations as cell loss or dilution of intracellular ...

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In vivo MRI tracking of iron oxide nanoparticle-labeled human mesenchymal stem cells in limb ischemia

In vivo MRI tracking of iron oxide nanoparticle-labeled human mesenchymal stem cells in limb ischemia

... of stem cells into target tissues results in the death of a percentage of the donor cells, 31,32 which may happen more often at the center of transplanted cells because of the lack of culture medium, as in a ...

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In-vitro labelling of ovine adipose-derived mesenchymal stem cells (oADMSCS) and tracking using MRI technique

In-vitro labelling of ovine adipose-derived mesenchymal stem cells (oADMSCS) and tracking using MRI technique

... successful stem cell-based therapy, the monitoring of transplanted cell’s migration, homing as well as the engraftment efficiency and functional capability in-vivo has become a critical ...

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Assessing the Efficacy of Nano- and Micro-Sized Magnetic Particles as Contrast Agents for MRI Cell Tracking

Assessing the Efficacy of Nano- and Micro-Sized Magnetic Particles as Contrast Agents for MRI Cell Tracking

... time, cell line, ...from cell proliferation to gene expression and DNA ...of stem cells: self-renewal and the capacity to give rise to specialised cells, and whether contrast agent labelling affected ...

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52Mn Production for PET/MRI Tracking Of Human Stem Cells Expressing Divalent Metal Transporter 1 (DMT1)

52Mn Production for PET/MRI Tracking Of Human Stem Cells Expressing Divalent Metal Transporter 1 (DMT1)

... Immunocytochemistry was used to examine ex- pression of the human DMT1 protein in hNPC. Cover slips were fixed with 4% paraformaldehyde (PFA) and washed with phosphate-buffered saline (PBS) before incubation in blocking ...

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Ex vivo MRI cell tracking of autologous mesenchymal stromal cells in an ovine osteochondral defect model

Ex vivo MRI cell tracking of autologous mesenchymal stromal cells in an ovine osteochondral defect model

... understanding MRI signal characteristics relating to particular events and have also demonstrated evidence of engraftment of stem cells to the defect site by MRI ...veterinary MRI scanner and ...

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Long-term MRI tracking of dual-labeled adipose-derived stem cells homing into mouse carotid artery injury

Long-term MRI tracking of dual-labeled adipose-derived stem cells homing into mouse carotid artery injury

... goal. MRI has the advantages of high temporal and spatial resolution and sharp contrast, 9 and has an ability to monitor the migration and homing of transplanted cells to injured tissues and organs in ...26 ...

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Development and Optimization of 19F-MRI for Tracking Cellular Therapeutics

Development and Optimization of 19F-MRI for Tracking Cellular Therapeutics

... with MRI,” Topics in Magnetic Resonance Imaging 25(5): ...presumed stem cells for the skeletal tissues of bone, cartilage, muscle and ...investigated stem-like cells in clinical ...tissues, ...

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