SV40 DNA
Interaction of the Transcription Factor TFIID with Simian Virus 40 (SV40) Large T Antigen Interferes with Replication of SV40 DNA In Vitro
... the SV40 core origin and also physically interacts with T antigen, we examined whether TBP complexes might stimulate SV40 DNA replication in ...support SV40 DNA replication in vitro, ...
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An N-terminal deletion mutant of simian virus 40 (SV40) large T antigen oligomerizes incorrectly on SV40 DNA but retains the ability to bind to DNA polymerase alpha and replicate SV40 DNA in vitro.
... shift DNA-binding experiments showed a reduction in the site II binding activity of T83-708 ...origin DNA–T83-708 complexes ap- peared to contain more than 12 monomers ...origin DNA binding, but this ...
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Mechanisms of interference with simian virus 40 (SV40) DNA replication by trans-dominant mutants of SV40 large T antigen.
... By cotransfecting CV-1 cells with an origin-defective plasmid encoding wild-type T antigen and a replicationcompetent plasmid encoding mutant T antigen, we saw that the level of interfer[r] ...
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Simian virus 40 (SV40) small t antigen inhibits SV40 DNA replication in vitro.
... This dramatic inhibition by small t depends on the presence of large T during the preincubation period; when extracts were preincubated with small t alone and large T was added at the be[r] ...
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Natural Isolates of Simian Virus 40 from Immunocompromised Monkeys Display Extensive Genetic Heterogeneity: New Implications for Polyomavirus Disease
... pathogen. SV40 DNA has recently been detected by PCR in human tumors, including pediatric and adult brain tumors (3, 21, 26), mesotheliomas (6–8, 30), and osteosarcomas (7, ...of SV40 association ...
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Use of Nondefective Adenovirus-Simian Virus 40 Hybrids for Mapping the Simian Virus 40 Genome
... The order of these early SV40 antigen inducing regions in the SV40 DNA segments contained in the nondefective hybrids is: U antigen, tumor specific transplantation antigen, and T antigen[r] ...
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SV40-Immortalized Human Fibroblasts as a Source of SV40 Infectious Virions
... that SV40 can establish a persistent infec- tion in human fibroblasts lasting after the “crisis” ...the SV40 genome characterized in MRC5-SV2 immortalized cells is wild-type and indistinguishable from the ...
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Purification of the simian virus 40 (SV40) T antigen DNA-binding domain and characterization of its interactions with the SV40 origin.
... of DNA replication is a complicated process that requires very precise protein-DNA interactions at replication origins (1, ...(SV40) DNA has served as a useful model system for studying ...
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Efficient Transcription of a Compact Nucleoprotein Complex Isolated from Purified Simian Virus 40 Virions
... Due to the relative simplicity of simian virus 40 SV40 nucleoprotein complexes and the ability to isolate SV40 DNA, DNA-histone minichromosome, and DNA-histone viral protein core complex[r] ...
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Three domains in the simian virus 40 core origin orchestrate the binding, melting, and DNA helicase activities of T antigen.
... Simian virus 40 large tumor antigen requires three core replication origin domains for DNA unwinding and replication in vitro.. Simian virus 40 SV40 DNA replication: SV40 large T antigen[r] ...
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Structural topography of simian virus 40 DNA replication.
... Isolated nuclei as well as nuclear matrices were able to continue SV40 DNA elongation under replication conditions in situ, arguing for a coordinated and functional association of SV40 D[r] ...
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Simian Virus 40 Deoxyribonucleic Acid Transcription In Vitro: Binding and Transcription Patterns with a Mammalian Ribonucleic Acid Polymerase
... It was found that i the majority of supercoiled SV40 DNA molecules displayed a single binding site for the enzyme, ii the supercoiled structure of SV40 DNA was frequently retained during[r] ...
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Ultraviolet irradiation inhibits encapsidation of simian virus 40 chromatin.
... To provide the infornation on maturation essential for analyzing SV40 DNA replication and repair in UV-irradiated cells, we have examined the progression of SV40 DNA along the maturation[r] ...
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Structure of Replicating Simian Virus 40 Deoxyribonucleic Acid Molecules
... We have examined SV40 RI and have observed that, during alkaline sedimentation, newly replicated SV40 DNA is not covalently attached to the parental DNA strand.. By electron microscopy o[r] ...
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Purification and functional properties of simian virus 40 large and small T antigens overproduced in insect cells.
... Moreover, SF9 T had ATPase activity comparable to that of T produced in monkey cells, exhibited helicase activity and SV40 origin-specific DNA binding, and was active in the SV40 DNA rep[r] ...
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Studies of Nondefective Adenovirus 2-Simian Virus 40 Hybrid Viruses VIII. Association of Simian Virus 40 Transplantation Antigen with a Specific Region of the Early Viral Genome
... Since it is likely that, in the inoculated hamsters, transcription of the SV40 DNA segments in the nondefective Ad2-SV40 hybrids is the same as transcription of these segments in lytical[r] ...
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cdc2 phosphorylation of threonine 124 activates the origin-unwinding functions of simian virus 40 T antigen.
... Mutations in the phosphorylation sites of simian virus 40 SV40 T antigen alter its origin DNA-binding specificity for sites I and II and affect SV40 DNA replication activity.. Binding of[r] ...
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The finger domain of simian virus 40 large T antigen controls DNA-binding specificity.
... Mutations in the phosphorylation sites of simian virus 40 SV40 T antigen alter its origin DNA-binding specificity for sites I and II and affect SV40 DNA replication activity.. Initiation[r] ...
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Simian virus 40 (SV40) T antigen binds specifically to double-stranded DNA but not to single-stranded DNA or DNA/RNA hybrids containing the SV40 regulatory sequences.
... We previously reported 28 that only a very small fraction of T Ag binds with a higher salt-sensitive affinity to SV40 DNA than to calf thymus DNA-cellulose under these conditions and tha[r] ...
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The N-terminal side of the origin-binding domain of simian virus 40 large T antigen is involved in A/T untwisting.
... in SV40 DNA replica- tion. To initiate DNA replication, T antigen must interact with three distinct DNA elements at the SV40 replication origin (12–14, 43): a central palindrome that ...
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