SYBR Green I
Development of a duplex SYBR Green I based quantitative real time PCR assay for the rapid differentiation of goose and Muscovy duck parvoviruses
... Despite the prophylaxis taken against waterfowl parvo- viruses, parvovirus outbreaks persist in coastal China where vaccination against parvoviruses is widely used in commercial goose and duck flocks [4, 24, 25, 27]. ...
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Direct comparison of the histidine rich protein 2 enzyme linked immunosorbent assay (HRP 2 ELISA) and malaria SYBR green I fluorescence (MSF) drug sensitivity tests in Plasmodium falciparum reference clones and fresh ex vivo field isolates from Cambodia
... non-specific SYBR Green fluorescence I is expected to improve MSF assay ...of SYBR Green I dye and other modifications that could improve performance of the assay in field ...
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TANDEM RECOMBINANT PLASMID CONSTRUCTION AS POSITIVE CONTROL FOR PIK3CA H1047R DETECTION BASED ON SYBR GREEN I qPCR
... PIK3CA H1047R mutation is found in breast cancer in high frequency and its detection could be applied as prognosis and predictive factor for trastuzumab therapy. qPCR is one of the simplest and robust method for PIK3CA ...
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A robust and versatile signal-on fluorescence sensing strategy based on SYBR Green I dye and graphene oxide
... Figure 7 Fluorescence spectra from “a” to “e” for the addition of 20 nM target, one-based mismatch, three-based mismatch, non-complementary MDr1 gene and without any target in the test conditions of 8 µl sgI, 50 nM cP, ...
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Real time quantitative PCR with SYBR Green I detection for estimating copy numbers of nine drug resistance candidate genes in Plasmodium falciparum
... on SYBR Green I detection and can, thus, be adapted to any given gene provided optimal experimental conditions are ...expensive SYBR green I detection ...
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Development and application of SYBR Green I real time PCR assay for the separate detection of subgroup J Avian leukosis virus and multiplex detection of avian leukosis virus subgroups A and B
... the SYBR Green I real-time PCR assay is relatively inexpensive and more convenient in that it does not require a specific probe but rather requires specific ...
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Simultaneous detection of porcine pseudorabies virus, porcine parvovirus and porcine circovirus type 2 by multiplex real-time PCR and amplicon melting curve analysis using SYBR Green I
... a SYBR Green I-based multiplex real-time PCR with melting curve analysis for the simultaneous detection of PPV, PRV and PCV2 ge- nomes in a single reaction vessel was developed and validated by ...
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Development of a SYBR green I based RT PCR assay for yellow fever virus: application in assessment of YFV infection in Aedes aegypti
... a SYBR Green I based qRT-PCR ...independent SYBR Green-I based qRT-PCR assay has the potential to detect such mutant strains ...
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A duplex, SYBR Green I based RT qPCR assay for the simultaneous detection of Apple chlorotic leaf spot virus and Cherry green ring mottle virus in peach
... [7-9]. SYBR Green-I multiplex RT-qPCR assays were developed for the simultaneous detection and quantification of animal viruses [13,14], and demon- strated that this strategy provides a reliable ...
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Development of Group and Serotype Specific One Step SYBR Green I Based Real Time Reverse Transcription PCR Assay for Dengue Virus
... from SYBR Green I-based real-time RT-PCR in daily routine screening, both flavivirus-specific (FL-F, GCC ATA TGG TAC ATG TGG; FL-R, TGT CCC ATC CTG CGG TAT CAT [200 to 100 nM]) and dengue-specific ...
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Measurement of Epstein Barr virus DNA load using a novel quantification standard containing two EBV DNA targets and SYBR Green I dye
... Several methods for quantifying absolute DNA load have been developed since its first application to EBV diagnos- tics in 1999 [18-20]. These include semi-quantitative, quantitative competitive and real-time PCR methods ...
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The development of a rapid SYBR Green I based quantitative PCR for detection of Duck circovirus
... A SYBR Green I-based quantitative PCR is an excellent diagnostic tool with high sensitivity, specificity, and a fast turnaround time [23,24]. This system is called real-time PCR because the ...
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Detection of mixed infection level of Plasmodium falciparum and Plasmodium vivax by SYBR Green I based real time PCR in North Gondar, north west Ethiopia
... Methods: The study was conducted in seven health centres from North Gondar, north-west Ethiopia. The data of all febrile patients, who attended the outpatient department for malaria diagnosis, from October to December ...
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Development of a SYBR Green I real-time PCR for the detection of the orf virus
... our SYBR Green I real-time PCR assay exhibits high specificity for detecting ORFV; among common DNA pathogens, only ORFV returned a positive ...
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Frequency of Human Herpesvirus 6 (HHV-6) in Pterygium Using Real-Time PCR Based on SYBR-Green I Fluorescence
... Methods: Fifty tissue specimens were collected from patients with pterygium who underwent pterygium surgery between February 2013 and May 2015. The specimens were tested by real-time PCR using Maxima SYBR ...
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Molecular detection of human rhinoviruses in respiratory samples: a comparison of Taqman probe , SYBR green I and BOXTO based real time PCR assays
... the SYBR green assay is associated with high specificity and positive predictive ...the SYBR Green and a Texas-Red labelled ...and SYBR Green-based detections in a combined assay ...
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The source of SYBR green master mix determines outcome of nucleic acid amplification reactions
... of SYBR green I is its lack of specificity: bind- ing to nonspecific dsDNA in the real-time PCR reaction hampers reliable quantification of the specific product ...
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High resolution melting as an alternative method to genotype diacylglycerol O acyltransferase 1 (DGAT1) K232A polymorphism in cattle
... RT-PCR amplification and HRM analysis Optimization of the PCR conditions and moni- toring of amplification efficiency in real time were carried out using the RotorGene™ 6000 (Corbett Life Science). Oligo 5.0 software was ...
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Drying anti malarial drugs in vitro tests to outsource SYBR green assays
... of SYBR Green I protocol in field laboratories with limited re- ...The SYBR Green I modified protocol described in this study enables to dry and store the plates, which will be ...
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A Real-time Resistance Measurement for DNA Amplification and Detection
... Five dyes (1: Crytal violet, 2: Methylene blue, 3: SYBR Green I, 4: Hydroxy naphthol blue, 5: Ethidium bromide) were applied in the same concentration (1.0mg.L -1 ) to compares of their reaction ...
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