SYBR Green I dye
A robust and versatile signal-on fluorescence sensing strategy based on SYBR Green I dye and graphene oxide
... Abstract: A robust and versatile signal-on fluorescence sensing strategy was developed to provide label-free detection of various target analytes. The strategy used SYBR Green I dye and ...
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Measurement of Epstein Barr virus DNA load using a novel quantification standard containing two EBV DNA targets and SYBR Green I dye
... Master SYBR Green 1 dye (Roche Diagnostics, Castle Hill, NSW, Australia) at 10× concentration pre- combined with the LightCycler® FastStart enzyme, ...
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Direct comparison of the histidine rich protein 2 enzyme linked immunosorbent assay (HRP 2 ELISA) and malaria SYBR green I fluorescence (MSF) drug sensitivity tests in Plasmodium falciparum reference clones and fresh ex vivo field isolates from Cambodia
... malaria SYBR Green I fluorescence (MSF) assay ...fluorescent SYBR Green I dye into parasite ...to SYBR Green I binding to human DNA of white blood ...
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The development of a rapid SYBR Green I based quantitative PCR for detection of Duck circovirus
... on SYBR Green I dye was ...a SYBR Green I-based quantitative PCR can therefore be practically used as an alternative diagnostic tool for duck circovirus ...
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High resolution melting as an alternative method to genotype diacylglycerol O acyltransferase 1 (DGAT1) K232A polymorphism in cattle
... However, the PCR amplification was successfully performed using lower concentrations (1.5µM) of SYBR Green I dye. Homozygous samples (AA and KK) could often be genotyped by an absolute change ...
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Real time quantitative PCR with SYBR Green I detection for estimating copy numbers of nine drug resistance candidate genes in Plasmodium falciparum
... with SYBR green I ...1). SYBR Green I fluorescent dye has the important property of being a sequence-independent, universal RT-qPCR detection system, due to its ability to ...
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Evaluating an MRNA based body fluid identification test using sybr green fluorescent dye and real time PCR
... The main advantage of a SYBR Green ® test over a Taqman ® based test is that it can be more cost-effective in certain circumstances. If mRNA profiling is to be utilised in forensic casework then the ...
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Adaptation of a visualized loop mediated isothermal amplification technique for field detection of Plasmodium vivax infection
... acid dye SYBR Green I [14], which, unfortunately, inhibits the amplification reaction, so cannot be added prior to the ...fluorescence dye is often added after the reac- tion has ...
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Flow cytometric quantification, sorting and sequencing of methanogenic archaea based on F420 autofluorescence
... with SYBR Green I, which was used to differentiate cells from the background noise and to further segregate the autofluorescent community F420+ into F420 + S1 and F420 + ...acid dye led to a ...
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Absolute quantitative real-time polymerase chain reaction for the measurement of human papillomavirus E7 mRNA in cervical cytobrush specimens
... the SYBR Green I (Molecular Probes, Eugene, OR) fluorescent intercalation ...iQ SYBR Green Supermix (Bio-Rad Laboratories, Hercules, CA), ...
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Comparison of Three Terminal Detection Methods Based on Loop Mediated Isothermal Amplification (LAMP) Assay for Spring Viremia of Carp Virus (SVCV)
... fluorescent dye (SYBR Green I) staining can substitute for gel electrophoresis to judge LAMP products by visual inspection even if its detection sensitivity was lower an order of ...the ...
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Development of a SYBR Green I real-time PCR for the detection of the orf virus
... Orf is a non-systemic, ubiquitous disease of sheep and goats caused by the orf virus (ORFV). ORFV occasionally causes cutaneous lesions in humans in contact with infected animals. In the present study, a real-time PCR ...
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TANDEM RECOMBINANT PLASMID CONSTRUCTION AS POSITIVE CONTROL FOR PIK3CA H1047R DETECTION BASED ON SYBR GREEN I qPCR
... Shift SYBR Green I qPCR) showed consistent results that the tandem positive control had been successfully construc- ...Using SYBR Green I as intercalating dye gene- rates ...
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Development of Mitochondrial Loop Mediated Isothermal Amplification for Detection of the Small Liver Fluke Opisthorchis viverrini (Opisthorchiidae; Trematoda; Platyhelminthes)
... LAMP test with field samples collected from fish and hu- mans. The specific LAMP assay was used in attempts to detect O. viverrini metacercariae and cysts from silver carp and to detect eggs from feces of humans in an ...
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Detection and Genotyping of Oocysts of Cryptosporidium parvum by Real Time PCR and Melting Curve Analysis
... Differentiation between C. parvum type 1 and type 2 by real-time PCR and with fluorescent probes. A pair of probes 24 and 25 bp in length, respectively, were tested in a real-time PCR format with the same btub1 and btub2 ...
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Examination of oral cancer biomarkers by tissue microarray analysis.
... Differential gene expression of CDH11, MMP3, SPARC, POSTN, TNC, and TGM3 104.. between normal and cancer specimens was quantified by using SYBR ® Green I technology and 105.[r] ...
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Rumpff, Dirk (2013): Perioperative Analyse initialer intra- und extrazellulärer Aktivierungsfaktoren bei Patienten mit kolorektalem Adenokarzinom. Dissertation, LMU München: Medizinische Fakultät
... Die einfachste Möglichkeit zur Quantifizierung der PCR-Produkte ist dabei die Nutzung von DNA-Farbstoffen (z. Ethidiumbromid oder SYBR Green I). Diese Fluoreszenzfarbst[r] ...
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Rapid Visual Detection of Highly Pathogenic Streptococcus suis Serotype 2 Isolates by Use of Loop Mediated Isothermal Amplification
... Streptococcus suis serotype 2 (S. suis 2) is an important zoonotic pathogen that causes considerable economic losses to the pig industry and significantly threatens public health worldwide. The highly pathogenic S. suis ...
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Simultaneous detection of porcine pseudorabies virus, porcine parvovirus and porcine circovirus type 2 by multiplex real-time PCR and amplicon melting curve analysis using SYBR Green I
... a SYBR Green I-based multiplex real time PCR assay for simultaneous detection of porcine parvovirus, porcine pseudorabies virus and porcine circovirus type ...
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Frequency of Human Herpesvirus 6 (HHV-6) in Pterygium Using Real-Time PCR Based on SYBR-Green I Fluorescence
... Real-time PCR was performed in an ABI system 7300 with Maxima SYBR Green/ROX qPCR Master Mix (2X) (Ampliqon, Denmark) kit according to the manufacturer’s instructions (15). The primer pair 5'- ...
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