TaqMan-based real-time PCR
Field Validation of SYBR Green and TaqMan Based Real Time PCR Using Biopsy and Swab Samples To Diagnose American Tegumentary Leishmaniasis in an Area Where Leishmania (Viannia) braziliensis Is Endemic
... and TaqMan- based real-time PCR (qPCR) assays with that of a composite reference standard con- sisting of the Montenegro skin test, serology, histopathology, smears, culture, and ...
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Development of a novel TaqMan based real time PCR assay for the detection of porcine boca like virus (Pbo likeV)
... specific TaqMan-based real-time PCR assay to target the Pbo-likeV NP1 ...reliable real-time tool for epidemiological and pathogenetic study of Pbo-likeV ...
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Establishing a TaqMan Based Real Time PCR Assay for the rapid detection and quantification of the newly emerged duck tembusu virus
... In April 2010, an outbreak of the newly emerged duck Tembusu virus (DTMUV) caused a range of symptoms in infected ducks, including retarded growth, high fever, loss of appetite, decreased egg production, and death. The ...
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Rapid detection of porcine circovirus type 2 using a TaqMan based real time PCR
... The standard PCV2 plasmid with 10 7 , 10 5 and 10 3 copies was used to evaluate the coefficients of variation (CVs) of the real-time PCR. Intra- and inter-assay CVs for Ct values were both included. ...
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Comparison of a New Quantitative ompA Based Real Time PCR TaqMan Assay for Detection of Chlamydia pneumoniae DNA in Respiratory Specimens with Four Conventional PCR Assays
... the real-time PCR technology is an additional diagnostic tool that offers numer- ous advantages such as an automated, nonnested format in- cluding template inactivation and the combination of ...
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Real Time Quantitative PCR Assays for Detection and Monitoring of Pathogenic Human Viruses in Immunosuppressed Pediatric Patients
... 23 real-time PCR assays based on TaqMan technology has been developed for the detection and monitoring of 16 different viruses and virus families including human polyomaviruses BK virus ...
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A simplified duplex real time PCR incorporating TaqMan minor groove binder (MGB) probes and an exogenous internal positive control for the simultaneous detection of Mycoplasma gallisepticum and Mycoplasma synoviae cultures
... duplex TaqMan labelled probe-based RT-PCR assay for detection of MG (mgc2 gene) and MS (vlhA gene) reported by Sprygin et ...1. TaqMan duplex real-time PCR show- ing ...
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Real Time PCR Based Pathotyping of Newcastle Disease Virus by Use of TaqMan Minor Groove Binder Probes
... a PCR targeting a more conserved region of the genome ...the PCR specific for velogens/mesogens (Table 3), in agreement with the definition of virus virulence by the OIE ...the time course ...
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Quantitative detection of human Malawi polyomavirus in nasopharyngeal aspirates, sera, and feces in Beijing, China, using real time TaqMan based PCR
... the time of year when multiple viruses circulate, and a history of immunosuppression are associated with an increased chance of viral co-infection ...China, based on a real-time qPCR specific ...
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Evaluation of Transported Dry and Wet Cervical Exfoliated Samples for Detection of Human Papillomavirus Infection
... by PCR amplification followed by dot blot hybridization ...of PCR products was then dotted onto nylon filters and probed with biotin-labeled -globin and generic HPV probes ...QIAquick PCR ...
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TaqMan Real Time Reverse Transcription PCR Assay for Universal Detection and Quantification of Avian Hepatitis E Virus from Clinical Samples in the Presence of a Heterologous Internal Control RNA
... the PCR assay than in vitro-transcribed RNA ...is based on RNA and could easily be adapted and integrated into the assay to check for successful RNA extraction and ...
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The rtxA Toxin Gene of Kingella kingae: a Pertinent Target for Molecular Diagnosis of Osteoarticular Infections
... methods based on the rtxA toxin gene sequence to diagnose this ...Two real-time PCR assays (SYBR green and TaqMan chemistries) targeting this gene are ...and/or PCR (16S rRNA and ...
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Development and Evaluation of a Novel Single Nucleotide Polymorphism Real Time PCR Assay for Rapid Detection of Fluoroquinolone Resistant Mycoplasma bovis
... and time-consuming, especially for clinical isolates, and is rarely performed in mycoplasma diagnostic ...rapid real-time detection of resistant strains of ...novel TaqMan single- ...
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Rapid and Sensitive Routine Detection of All Members of the Genus Enterovirus in Different Clinical Specimens by Real Time PCR
... were based on highly conserved regions in the 5⬘-untranslated region of the enterovirus ge- ...2⫻ TaqMan Uni- versal PCR Master Mix, a 900 nM concentration of forward primer, 300 nM concentrations of ...
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Development of a TaqMan Array Card for Acute Febrile Illness Outbreak Investigation and Surveillance of Emerging Pathogens, Including Ebola Virus
... a real-time PCR-based TaqMan array card (TAC) that can test six to eight samples within ...individual real-time PCR assays, and the TAC exhibited an overall 88% ...
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Comparison of the Xpert MTB/RIF Test with an IS6110 TaqMan Real Time PCR Assay for Direct Detection of Mycobacterium tuberculosis in Respiratory and Nonrespiratory Specimens
... by PCR is highly dependent on the efficiency of DNA extraction procedures (4, 14, 20), which should eliminate inhibiting substances and achieve optimal ...IS6110- TaqMan assay may increase the release of ...
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Use of TaqMan Real Time Reverse Transcription PCR for Rapid Detection, Quantification, and Typing of Norovirus
... (RT)- PCR, a method that requires confirmation by probe or se- quence analysis (3, ...including PCR and probe hybridization with multiple genotype-specific oligonu- cleotides and SYBR green analysis (3, 4, ...
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Development of a duplex TaqMan real time RT PCR assay for simultaneous detection of newly emerged H5N6 influenza viruses
... designed based on the highly conservative regions of HA and NA genes of A/Duck/ Guangxi/1/18(H5N6) virus downloaded from GenBank by using the MegAlign module of the DNAStar ...
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Novel Method for Genotyping Hepatitis B Virus on the Basis of TaqMan Real Time PCR
... genotyping based on TaqMan real-time PCR, which identifies all HBV genotypes without post-PCR ...multiplex real-time PCRs, each targeting two or three ...
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Evaluation of Polymerase Chain Reaction and Cobas TaqMan Real Time PCR in the Diagnosis of Tuberculosis: Indian Prospective
... various PCR targets reliable screening test for tuberculosis in clinical specimen such as devR, TRC4, GCRS, MPB64 (MPt64), ...around time for definitive mycobacteriological detection in the laboratory to ...
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