TaqMan real-time PCR
Quantification of Human Immunodeficiency Virus Type 1 Proviral Load by a TaqMan Real Time PCR Assay
... by TaqMan real-time PCR. The principle of TaqMan real-time PCR is based on the cleavage of an internal probe by the 5⬘-3⬘ exonuclease activity of the Taq polymerase ...
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Evaluation of Polymerase Chain Reaction and Cobas TaqMan Real Time PCR in the Diagnosis of Tuberculosis: Indian Prospective
... of PCR for the detection of ...conventional PCR using two gene targets (IS6110 and MPB64). Results: The PCR positivity of IS6110 and MPB64 gene targets was found to be ...the PCR positivity ...
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Original Article Development of TaqMan real-time PCR for detection of stenotrophomonas maltophilia
... mention TaqMan real- time ...Man real-time PCR of ...the TaqMan real-time fluorescent quantitative PCR basing ordinary PCR is a new technique ...
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Novel Method for Genotyping Hepatitis B Virus on the Basis of TaqMan Real Time PCR
... on TaqMan real-time PCR, which identifies all HBV genotypes without post-PCR ...multiplex real-time PCRs, each targeting two or three genotype-specific segments of ...
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Original Article The impact of storage time on HCV RNA quantitative detection in serum samples by TaqMan real-time PCR
... with TaqMan real-time reverse tran- scription polymerase chain reaction (TaqMan real-time PCR) is considered to be more precise and sensitive than traditional anti-HCV ...
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Comparison of the Xpert MTB/RIF Test with an IS6110 TaqMan Real Time PCR Assay for Direct Detection of Mycobacterium tuberculosis in Respiratory and Nonrespiratory Specimens
... of PCR technologies has been achieved with the development of real-time PCR testing ...of real-time PCR are a shortened turnaround time; automation of the ...
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A TaqMan real time PCR assay for Rhizoctonia cerealis and its use in wheat and soil
... Amount of Rhizoctonia cerealis DNA detected pg/g soil in 92 UK field soils under different crops at two time points spring and autumn Previous crop.. Spring Allium Oilseed Rape Pasture P[r] ...
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A TaqMan real time PCR assay for the detection and quantitation of Plasmodium knowlesi
... nested PCR and/or sequencing [2,22], LAMP [23], and real-time PCR ...countries, real-time PCR is the method of choice by providing superior sensitivity, rapid results and ...
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A simplified duplex real time PCR incorporating TaqMan minor groove binder (MGB) probes and an exogenous internal positive control for the simultaneous detection of Mycoplasma gallisepticum and Mycoplasma synoviae cultures
... duplex TaqMan real-time PCR assay for the simultaneous detection of MG and ...duplex real-time PCR assay incorporates TaqMan (FAM/NED) labelled minor groove binder ...
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Multicenter Performance Evaluation of a New TaqMan PCR Assay for Monitoring Human Immunodeficiency Virus RNA Load
... A TaqMan real-time PCR assay, the COBAS TaqMan human immunodeficiency virus (HIV) (HPS-CTM HIV) PCR assay, recently developed for the quantification of HIV type 1 RNA in plasma, ...
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Detection and Genotyping of Varicella Zoster Virus by TaqMan Allelic Discrimination Real Time PCR
... a TaqMan real-time PCR assay to detect and differentiate wild-type virus from Oka vaccine strains of ...to real-time monitoring of PCR product formation and allelic ...
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Rapid detection of porcine circovirus type 2 using a TaqMan based real time PCR
... The standard PCV2 plasmid with 10 7 , 10 5 and 10 3 copies was used to evaluate the coefficients of variation (CVs) of the real-time PCR. Intra- and inter-assay CVs for Ct values were both included. ...
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Evaluation of a Multiplex Real Time Reverse Transcriptase PCR Assay for Detection and Differentiation of Influenza Viruses A and B during the 2001 2002 Influenza Season in Israel
... multiplex real-time reverse transcriptase PCR (RT-PCR) assay (which uses TaqMan chemistry) and evaluated it for its ability to detect and concomitantly differentiate influenza viruses A ...
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Rapid and Sensitive Routine Detection of All Members of the Genus Enterovirus in Different Clinical Specimens by Real Time PCR
... the real-time TaqMan PCR (Table ...our PCR, with multiple primers and probes, detected all 60 different enterovirus species ...rhinovirus real-time TaqMan ...
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Evaluation of Transported Dry and Wet Cervical Exfoliated Samples for Detection of Human Papillomavirus Infection
... by PCR amplification followed by dot blot hybridization ...of PCR products was then dotted onto nylon filters and probed with biotin-labeled -globin and generic HPV probes ...QIAquick PCR ...
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Development of a duplex TaqMan real time RT PCR assay for simultaneous detection of newly emerged H5N6 influenza viruses
... duplex real-time RT- PCR assay was developed to identify H5N6 subtype ...hands-on time. Contrasting to single probe rRT- PCR, this method introduced two different labeled probes so that ...
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Development of TaqMan® MGB fluorescent real time PCR assay for the detection of anatid herpesvirus 1
... The PCR is a rapid, sensitive and specific nucleic acid amplification technique and many conventional qualita- tive PCR methods for revealing merely the presence or absence of AHV-1 pathogen have been ...
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Real Time Quantitative PCR Assays for Detection and Monitoring of Pathogenic Human Viruses in Immunosuppressed Pediatric Patients
... 23 real-time PCR assays based on TaqMan technology has been developed for the detection and monitoring of 16 different viruses and virus families including human polyomaviruses BK virus and JC ...
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Molecular Detection of Linezolid Resistance in Enterococcus faecium and Enterococcus faecalis by Use of 5′ Nuclease Real Time PCR Compared to a Modified Classical Approach
... Taqman PCR. Homozygous linezolid-susceptible and -resis- tant isolates, including E. faecium ATCC 19434 (susceptible), E. faecium 3939 (resistant), E. faecalis V583 (susceptible), and E. faecalis 3696 ...
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TaqMan Real Time Reverse Transcription PCR Assay for Universal Detection and Quantification of Avian Hepatitis E Virus from Clinical Samples in the Presence of a Heterologous Internal Control RNA
... the TaqMan duplex real-time RT-PCR presented here uses newly developed prim- ers and probe binding within ORF3 of the avian HEV genome and is suitable for universal ...of ...
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