Targeted gene disruption using a suicide vector
Targeted gene disruption in Xenopus laevis using CRISPR/Cas9
... geted gene disruption in Xenopus laevis , we targeted ptf1a/p48 and tyrosinase in this species and found that in addition to high sequence disruption efficiency, clear phenotypes were observed ...
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Investigation of annexin function by targeted gene disruption
... cells using the now well-documented method of homologous recombination, and genetically modified ES cells, identified by PCR, were then injected into blastocysts and reimplanted into recipient ...
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Targeted Gene Disruption by Ectopic Induction of DNA Elimination in Tetrahymena
... somatic gene KO strain, all copies of a target gene in the polyploid MAC must be ...target gene or by coDel of some copies of a target gene followed by random assortment of MAC chromo- somes ...
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Targeted disruption of the Kvlqt1 gene causes deafness and gastric hyperplasia in mice
... Inner ear histology. The histology of the cochlea and vestibular organs of the inner ears was studied in a Kvlqt1 +/+ mouse of approximately 4 months of age and in a Kvlqt1 –/– mouse of the same age. After removal, the ...
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Adeno associated virus–targeted disruption of the CFTR gene in cloned ferrets
... cell gene targeting combined with nuclear transfer cloning presents tremendous potential for the creation of new, large-animal models of human ...CFTR gene–deficient model in the domestic ferret ...
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Development of the CRISPR/Cas9 System for Targeted Gene Disruption in Aspergillus fumigatus
... making gene silencing complete and ...ciency gene disruption in A. fumigatus, using the pksP gene (essen- tial for melanin production) as a case study (30, ...
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Lack of anabolic response to skeletal loading in mice with targeted disruption of the pleiotrophin gene
... showed, using the whole genome microarray approach, that pleiotrophin (PTN) expression was increased by 4-fold in response to mechanical loading (ML) in a good responder C57BL/6J (B6) ...PTN gene ...
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Targeted disruption of the Chop gene delays endoplasmic reticulum stress–mediated diabetes
... studies using genetically engi- neered mice revealed that PERK disruption and defec- tive eIF2 α phosphorylation results in β cell insuffi- ciency, thereby demonstrating that the PERK-eIF2 α pathway plays ...
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Development of a Targeted Gene Vector Platform Based on Simian Adenovirus Serotype 24
... used vector prototype, human adenovirus serotype 5 ...target-specific gene delivery—justify the exploration of other Ad serotypes as vector ...alternative vector platform using simian ...
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Gene disruption/knock in analysis of mONT3: vector construction by employing both in vivo and in vitro recombinations
... by using the Red α / β -based in vivo recombination, one can isolate genomic fragments from the large collection of BAC libraries without the handling of BAC DNAs or the use of PCR, which may cause ...by ...
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Targeted Disruption of the Ceacam1(MHVR) Gene Leads to Reduced Susceptibility of Mice to Mouse Hepatitis Virus Infection
... The scheme for producing the targeting vector illustrated in Fig. 1C was designed to insert the pTK-neo r selection cassette (69) downstream of exon 2. Two major restriction fragments were used to prepare the ...
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Targeted gene disruption reveals a leptin independent role for the mouse beta3 adrenoceptor in the regulation of body composition
... Metabolic rate measurements. Evaluation of metabolic rates was performed on mice fed a chow diet. 8-h energy expenditure was mea- sured using open-circuit indirect calorimetry with computerized equipment. The ...
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Impaired degradation of inhibitory subunit of NF- B (I B) and -catenin as a result of targeted disruption of the -TrCP1 gene
... 1. Targeted disruption of the mouse -TrCP1 ...targeting vector (pFBW1KO), the mouse -TrCP1 gene locus, and the mutant allele resulting from homologous ...-TrCP1 gene is depicted by a ...
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Targeted gene disruption of matrix metalloproteinase 9 (gelatinase B) suppresses development of experimental abdominal aortic aneurysms
... Studies using animal models have led to important insights into the cellular and molecular events underly- ing aneurysmal degeneration ...with targeted gene deficiencies to evaluate the relative ...
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Selective Alterations in Organ Sizes in Mice with a Targeted Disruption of the Insulin-Like Growth Factor Binding Protein-2 Gene
... 1. Targeted Deletion of the IGFBP-2 Gene Panel A, Restriction map of the wild-type mouse IGFBP-2 genomic fragment containing exons 2, 3, and ...Replacement vector for deletion of the IGFBP-2 ...
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Efficient Gene Disruption in Diverse Strains of Toxoplasma gondii Using CRISPR/CAS9
... efficient gene disruption in a variety of ...for targeted gene disruption and for site-specific insertion of selectable markers through homologous ...isolate. Using CRISPR/CAS9, ...
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Seeking gene relationships in gene expression data using support vector machine regression
... We used mySVM [6] for SVM regression. Once the training data were formed, the target data were assigned either ran- domly or in a predetermined manner, depending on the search scenario used. The predicted results were ...
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Disruption of eyelid and cornea development by targeted overexpression of the glucocorticoid receptor
... ethanol. Embryos and newborn heads were embedded in paraffin as a whole. Consecutive 3 to 4 µm-thick sections were obtained. For histopa- thology, sections were routinely stained with hematoxylin/eosin. Embryos were ...
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Modification of Adenovirus Capsid with a Designed Protein Ligand Yields a Gene Vector Targeted to a Major Molecular Marker of Cancer
... Ad vector tropism will be successfully ...by using an affibody-modified, knob-deleted fiber fused with the neck region peptide of lung surfactant protein D have been unsuccessful ...
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BDNF gene methylation in brain of suicide subjects
... analysis using the sequencing primer provided by the LINE-1 assay reagent ...reactions using the following primers: BDNF FW2 5’- gggggaggattaaTtgagTTagttTtg -3’ (position from nucleotides -358 to -332) and ...
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