Tau aggregation
Potential synergy between tau aggregation inhibitors and tau chaperonemodulators
... non-native tau state is of great interest; however, at this time only one drug is entering phase III clinical trials for treating ...Generally, tau manipulation by therapeutics can either directly or ...
8
Tau Aggregation Correlates with Amyloid Deposition in Both Mild Cognitive Impairment and Alzheimer's Disease Subjects
... phosphorylated tau from AD brains into transgenic AD mice (3xTg; mutations to APP, Presenilin 1 and MAPT 1) not only induced tau aggregation and propagation, but also increased amyloid plaque load ...
29
Cerebrospinal fluid from Alzheimer’s disease patients promotes tau aggregation in transgenic mice
... stage tau hyperphosphoryla- tion and tau aggregation by the AD CSF tau seeds, as also visible on higher magnification views of the CA1 region ...positive tau structures became ...
9
Preferential tau aggregation in von Economo neurons and fork cells in frontotemporal lobar degeneration with specific MAPT variants
... aggregate tau compared to neighboring ...of tau hyperphosphorylation in the patients with the V337 M variant ...lated tau revealed that tau aggregates typically filled the VEN and fork cell ...
10
Norepinephrine metabolite DOPEGAL activates AEP and pathological Tau aggregation in locus coeruleus
... triggers Tau aggregation in vitro and in ...modifies Tau, we incubated recombinant WT Tau with DOPEGAL for 24 ...induced Tau aggregation, indicated by the accu- mulation of ...
17
Metformin promotes tau aggregation and exacerbates abnormal behavior in a mouse model of tauopathy
... with or without metformin. Caspase 3 total protein expression was similar across experimental groups (caspase/tubulin. P301S: Ctr, 0.2 ± 0.06; Met, 0.2 ± 0.03, P = 0.6, Student t- test). Consistent with previous find- ...
20
Amyloid β-protein oligomers promote the uptake of tau fibril seeds potentiating intracellular tau aggregation
... protein aggregation of tau or α-synuclein was quantified by FRET-based flow ...protein aggregation was conducted for a minimum of three independent experiments with at least three repli- cates in ...
13
Effect of pseudophosphorylation and cross-linking by lipid peroxidation and advanced glycation end product precursors on Tau aggregation and filament formation
... triple Tau mutant S396D/T403E/S404D reacts slightly faster and forms more ThT aggregates in the reaction with acrolein compared with wild-type Tau ...higher aggregation rate in the triple mutant than ...
8
ER stress in Alzheimer's disease: a novel neuronal trigger for inflammation and Alzheimer's pathology
... phosphorylate tau protein and trigger aggregation of neurofibrillary tangles [161,162], and (iii) potentiate inflammatory responses via AP-1 activation ...of tau pro- tein [166,167], which are ...
13
Ischemic axonal injury up-regulates MARK4 in cortical neurons and primes tau phosphorylation and aggregation
... μl/well). Tau repeat domain (residues 244–372), expressed as previ- ously described [45], was aggregated until Thioflavin T fluorescence intensity reached a plateau, and was then diluted into Opti-MEM (GIBCO) and ...
12
Intersection of pathological tau and microglia at the synapse
... of tau contains two hexapeptides that can form intermolecular beta sheet rich structures: aa275– 280 (VCIINK) in R2 and aa306–311 (VQIVYK) in R3 [307, ...of tau can inter- act with physiological tau, ...
25
A seeding based cellular assay of tauopathy
... the tau protein typically does not form a well- ordered fibrilar structure in ...produce tau fibrils. One is through charge compensation by polyanions to form tau filaments from recombinant ...
10
Tau pathogenesis is promoted by Aβ1-42 but not Aβ1-40
... full-length tau can aggregate in certain cell type, SHSY5Y and ...of tau aggregation was not correlated with the level of tau expression because no aggregates formed in N2a cells at 24 h even ...
11
Synergistic influence of phosphorylation and metal ions on tau oligomer formation and coaggregation with α-synuclein at the single molecule level
... FCS and SIFT were performed on an InsightReader (Evotec-Technologies, Hamburg, Germany) with dual color excitation at 488 and 633 nm as described previ- ously [48,49,61]. Data was collected separately for each excitation ...
13
Distinct conformations, aggregation and neuronal internalisation of different tau strains
... of tau starts with verifiable plasmids as genetic constructs for overexpressing the protein in suitable model systems, with ...of tau, it was essential to use tau plasmids of similar ...forms ...
196
Distinct conformations, aggregation and cellular internalization of different tau strains
... misfolded tau (Arriagada et ...when tau proteins lose affinity for MTs and bind to themselves, forming insoluble aggregates that can subsequently develop into NFTs (Goedert, ...monomeric tau – the ...
16
Tumor suppressor PTEN affects tau phosphorylation: deficiency in the phosphatase activity of PTEN increases aggregation of an FTDP-17 mutant Tau
... human tau at multiple sites, decrease tau aggregation and improve tau binding to microtubules in cells ...that tau phosphorylation is harmful to neurons, these results sug- gest that ...
10
Enhancement of therapeutic potential of a naturally occurring human antibody targeting a phosphorylated Ser422 containing epitope on pathological tau
... the aggregation of tau, we used chemical ligation to prepare homogeneous tau with phosphoryl- ation at Ser 422 ...in tau, the oxi- dation state of which has an impact on aggregation; in ...
14
Preparation of stable tau oligomers for cellular and biochemical studies
... h. Two microliter aliquots were spotted onto a piece of nitrocellulose membrane, air dried, blocked with 10 % non-fat milk in phosphate buffered saline (PBS) containing 0.05 % Tween for 30 min at RT, and subsequently ...
28
Effects of the C57BL/6 strain background on tauopathy progression in the rTg4510 mouse model
... For tau protein analysis, 1-5 μg of the soluble fraction and 3 μl ...soluble tau and phospho-tau levels were quantified by normalizing protein levels to GAPDH ...of tau aggregated in the ...
20