Temperature-sensitive mutants
Preliminary Physiological Characterization of Temperature-Sensitive Mutants of Vesicular Stomatitis Virus
... Preliminary Physiological Characterization of Temperature-Sensitive Mutants of Vesicular Stomatitis Virus Different temperature-sensitive mutants of vesicular stomatitis virus have been [r] ...
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Isolation and characterization of temperature-sensitive mutants of adenovirus type 7.
... Intertypic complementation tests with temperature-sensitive mutants of adenovirus type 5 showed that the mutant in complementation group A failed to complement H5ts125 a DNA-binding prot[r] ...
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Isolation and Preliminary Characterization of Temperature-Sensitive Mutants of Rhizobiophage C
... Mutants were assigned to complementation groups by observing lysis at 29 C in bacteria mixedly infected with two temperature-sensitive mutants.. The mixture was left for 30 min for adsor[r] ...
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RNA Synthesis in Temperature-Sensitive Mutants of Vesicular Stomatitis Virus
... REICHMANN Department of Microbiology, University of Illinois, Urbana, Illinois 61801 T-particle-free stocks of temperature-sensitive mutants representing the four Glasgow complementation[r] ...
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Isolation and preliminary characterization of temperature-sensitive mutants of encephalomyocarditis virus.
... I have isolated temperature-sensitive mutants of encephalomyocarditis EMC virus because I wished to find mutants where the cleavage of viral precursor proteins is defective in mutant-inf[r] ...
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Isolation and Preliminary Characterization of Temperature-Sensitive Mutants of Influenza Virus
... Temperature-sensitive mutants of influenza A virus: isolation of mutants and preliminary observations on genetic recombination and complementation.. Influenza viral RNA and ribonucleopro[r] ...
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Physiological characterization of temperature-sensitive mutants of mengovirus.
... Cooper and co-workers 9-11 have described amount of viral RNA synthesized by cells infected with ts mutants have been observed with a class of ts mutants of poliovirus which is similar t[r] ...
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Characterization of temperature-sensitive mutants of Pichinde virus.
... In cells infected with ts2, all proteins were detectable; however, the amounts of NP, GPC, and L proteins detectable were reduced in cells incubated at the nonpermissive temperature as c[r] ...
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Temperature-sensitive Mutants of Sindbis Virus: Biochemical Correlates of Complementation
... In this report, we have shown that complementation between ts mutants is accompanied by three biochemical events: i an increase in the amount of mutant viral RNA that is converted to a r[r] ...
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Temperature-sensitive mutants of Japanese encephalitis virus.
... Monolayers were infected with virus at a multiplicity of 1 PFU per cell in M199 containing 7.5% bovine serum and 0.2 jLg of Act D per ml and incubated at the appropriate temperature.. At[r] ...
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Studies of temperature sensitive mutants of polyoma virus
... This conclusion may, however, · be invalid when the multiplicity of infection is high as in this experiment; the time of the temperature sensitiv:e step may be overestimated if, within t[r] ...
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Genetic and Physiological Properties of Temperature-Sensitive Mutants of Cocal Virus
... Complementation indices obtained in intersynthesis of viral RNA and protein at 39 C; TABLE and intrastrain mixed infection of VSV ts mutants furthermore, mutant ts G31 VSV Indiana, and m[r] ...
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Isolation and preliminary characterization of temperature-sensitive mutants of measles virus.
... Little or no infectious virus was produced in cells infected with group I or group III mutants and shifted to 33.5 C in the presence of 5-AC, indicating little or no virion RNA synthesis[r] ...
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Persistent Infections in L Cells with Temperature-Sensitive Mutants of Reovirus
... Some insight has thus been gained into the viral changes that occur in carrier cultures, but detailed studies with a variety of genetically 250 Downloaded from http://jvi.asm.org/ on Nov[r] ...
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MAPPING OF TEMPERATURE-SENSITIVE MUTANTS IN BACTERIOPHAGE T5
... Characterization of mutants: About one third of the ts mutants gave plaques resembling wild-type plaques in size. The remainder gave somewhat smaller plaques whether [r] ...
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INTRAGENIC COMPLEMENTATION AMONG TEMPERATURE SENSITIVE MUTANTS OF BACTERIOPHAGE T4D
... for example) which in some cases (the head protein for example) may be required in large amounts for phage assembly. Still other gene products may be required in amounts much lower th[r] ...
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Temperature-sensitive Mutants of Respiratory Syncytial Virus
... After ts mutants had been selected in BK tissue and stocks of virus prepared in these cells, further characterization was performed in a heteroploid cell line HeLa in which RS virus prod[r] ...
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AN ENRICHMENT FOR TEMPERATURE-SENSITIVE MUTANTS IN TETRAHYMENA THERMOPHILA
... We have isolated mutants that immediately stop DNA, as well as RNA and protein, synthesis upon shift-up to the restrictive temperature, and they are strongly enriched for b[r] ...
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TEMPERATURE-SENSITIVE MUTANTS OF BACTERIOPHAGE T4D: THEIR ISOLATION AND GENETIC CHARACTERIZATION
... mutants remains the same with increased temperature of incubation, but the plaques get smaller until a temperature is reached at which plaques can no longer be discerned. Th[r] ...
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Creation and characterization of temperature-sensitive mutants of the lck tyrosine protein kinase.
... Mutations identical to those responsible for the temperature-sensitive phenotypes of the tsNY68 and tsNY72-4 v-src mutants rendered this activated kck gene temperature sensitive for both[r] ...
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