The catalytic domain
Structure of the catalytic domain of the colistin resistance enzyme MCR-1
... periplasmic catalytic domain of the MCR-1 enzyme de- termined at ...MCR-1 catalytic domain is similar to that of the LptA and EptC transferases, as expected based on sequence homolo- ...the ...
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Modulation of the Allosteric Communication between the Polo-Box Domain and the Catalytic Domain in Plk1 by Small Compounds
... kinase domain indicated binding to the ...kinase domain and competes out AMP-PNP from its binding ...the catalytic domain, which could be modulated by small ...
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Structure-based mutagenesis of the catalytic domain of human immunodeficiency virus type 1 integrase.
... Minor dimer interface. Results of in vitro complementation experiments have shown that the catalytic domain plays an important role in the functional multimerization of HIV-1 IN (14, 45). There are two ...
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Isolation of Mutations in the Catalytic Domain of the Snf1 Kinase That Render Its Activity Independent of the Snf4 Subunit
... the catalytic domain? Jiang and Carlson have proposed a competitive binding model in which the autoinhibitory domain is bound to the catalytic domain under high-glucose conditions and ...
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In Silico Mapping of Essential Residues in the Catalytic Domain of PDE5 Responsible for Stabilization of Its Commercial Inhibitors
... In silico analyses have been applied recently in order to understand the interactions of several phosphodiesterase types and different inhibitors in the catalytic pocket. These are powerful methods for predicting ...
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Cloning and optimization of expression and purification of the catalytic domain of adenylate cyclase toxin from an Iranian strain of Bordetella pertussis
... Results: AC fragment was cloned successfully into a pET28a vector and under expression system with a concentration of 0.5 mM IPTG induction after 4 hours at 37 °C, AC can be expressed as a function of maximum yields ...
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Release of the catalytic domain N(o) from the herpes simplex virus type 1 protease is required for viral growth.
... the catalytic domain N o , was not sufficient to support the growth of m100, suggest- ing that an essential function of the protease between residues 419 and 514 was ...alytic domain N o from Pra is ...
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Expression in E coli and characterization of the catalytic domain of Botrytis cinerea chitin synthase
... SGC domain of CHS3a does not exhibit chitin synthase activity, specific binding of the UDP-GlcNAc substrate was clearly demonstrated and a higher affinity for the longer C-terminal SGC form was ...the ...
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Mutation of the Catalytic Domain of the Foamy Virus Reverse Transcriptase Leads to Loss of Processivity and Infectivity
... DISCUSSION The highly conserved YXDD motif contains two of the three aspartic acid residues that make up the RT active site (12, 14, 18, 23). The three-dimensional crystal structure of HIV-1 RT shows that the YXDD motif ...
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Replacement and deletion mutations in the catalytic domain and belt region of Aspergillus awamori glucoamylase to enhance thermostability
... The 23-30 replacement mutation increased specific activity slightly compared to wild-type GA and led to the most thermostable mutant GA constructed in this study, giving an approximatel[r] ...
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Three-dimensional structure of the catalytic domain of chitinase Al Bacillus circulars WI-12 at a very high resolution
... allosamidin homologue), as observed in the crystal structure of the demethylallosamidin complex (to be published). Comparison with the structure of hevamine. Am[r] ...
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Targeted DNA demethylation of the Arabidopsis genome using the human TET1 catalytic domain.
... the catalytic domain of the human demethylase TEN-ELEVEN TRANSLOCATION1 (TET1cd) and an artificial zinc finger (ZF) designed to target the FWA promoter can cause highly efficient targeted demethylation, FWA ...
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Somatic cancer mutations in the MLL3-SET domain alter the catalytic properties of the enzyme
... the catalytic domain of ...SET domain of MLL3 may lead to tumorigenesis through two converse ...the catalytic activity of MLL3, which re- sembles the effect of other loss of function mutations ...
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Crystal Structure of the Dengue Virus RNA-Dependent RNA Polymerase Catalytic Domain at 1.85-Angstrom Resolution
... palm domain appears to be the most structurally conserved among all known polymerases, reflecting the pres- ervation of the architecture of the catalytic site during evolu- ...the catalytic ...
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Severe Acute Respiratory Syndrome Coronavirus Papain-Like Protease Ubiquitin-Like Domain and Catalytic Domain Regulate Antagonism of IRF3 and NF-κB Signaling
... the catalytic domain in IFN ...the catalytic triad in the active site differentially affected IFN antagonism ...out catalytic activity (6), also affects the an- tagonism ...the ...
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RpfC (Rv1884) atomic structure shows high structural conservation within the resuscitation promoting factor catalytic domain
... the catalytic domain of RpfC (residues Gly68–Lys159 of UniProt RPFC_MYCTU) was cloned into the NdeI and NheI sites of the pET15-TEV plasmid to generate a recombinant protein containing a six-histidine tag ...
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A smallest 6 kda metalloprotease, mini-matrilysin, in living world: a revolutionary conserved zinc-dependent proteolytic domain- helix-loop-helix catalytic zinc binding domain (ZBD)
... We take some metalloproteases to compare with human MMP7 [3] as fallowing: ADAM [20], Astacin [21], Adamlysin II [22], P. aeruginosa alkaline protease [23], 2.4.24.11 protease [10]. It is clear to show that the loca- ...
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Evolving Catalytic Properties of the MLL Family SET Domain
... SET domain revealed an open conformation, which was suboptimal for methyl transfer to the target lysine ( Southall et ...SET domain confor- mation, thus stimulating ...SET domain ( Patel et ...SET ...
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Structure of the dengue virus helicase/nucleoside triphosphatase catalytic domain
... SUMMARY Dengue fever is an important emerging public health concern, with several million viral infections occurring annually, for which no effective therapy currently exists. The NS3 protein of Dengue virus is a ...
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Modulation of catalytic activity in multi-domain protein tyrosine phosphatases
... three-dimensional models of the DLAR and PTP99A PTP domains were examined using the GROMACS suite 4.0.7 (Groingen Machine for Chemical Simulations, [24]). In an effort to compare the PTP domains of DLAR and PTP99A ...
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