The metaphase mitotic spindle
Kinetochore dynamics and their attachment to the mitotic spindle
... 1.4. Kinetochore dynamics 1.4 Kinetochore dynamics A powerful way to understand kinetochore structure in relation to force generation and attachment state, is to investigate their dynamic behaviour and then define the ...
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Removal of antagonistic spindle forces can rescue metaphase spindle length and reduce chromosome segregation defects
... The mitotic spindle is a large structure responsible for the regulation and organization of chromosome ...the mitotic spindle and play several key roles during mitosis—mediating chromosomal ...
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Hsp72 is targeted to the mitotic spindle by Nek6 to promote K fiber assembly and mitotic progression
... Online supplemental material Fig. S1 shows purification of Nek6 substrates from HEK293 extracts using the KESTREL strategy. Fig. S2 shows additional data relating to mitotic phe- notypes associated with loss of ...
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Mechanics of Mitotic Spindle Poles and Polymerization Dynamics of Microtubules.
... that spindle poles and bivalents were mechanically connected by birefringent chromoso- mal fibers, which were relatively flexible but relatively inextensible and which buckled under compression [Begg and Ellis, ...
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Investigation of Force, Kinetochores, and Tension in the Saccharomyces Cerevisiae Mitotic Spindle
... cycle spindle pole bodies grow and nucleate ...the spindle pole body, they are curious if S phase-arrested spindle pole bodies can grown in size and nucleate more microtubules than previously seen ...
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Transient defects of mitotic spindle geometry and chromosome segregation errors
... the mitotic apparatus, and different types of kinetochore ...the spindle equator forming the metaphase plate, and the cell is said to be in ...the mitotic spindle in ...chromosomes. ...
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Regulated Assembly of the Mitotic Spindle: A Perspective from Two Ends
... for spindle assembly/maintenance but instead appear to generate pushing or polar ejection forces that promote chromosome movement away from the spindle ...the metaphase (or meiosis II ...
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Smurf2 as a novel mitotic regulator: From the spindle assembly checkpoint to tumorigenesis
... The spindle assembly checkpoint The spindle assembly checkpoint is a complex signal transduction cascade that inhibits the metaphase to ana- phase transition in the presence of unattached or untensed ...
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A shift from kinesin 5 dependent metaphase spindle function during preimplantation development in mouse
... that metaphase spindle bipolarity becomes independent of kinesin 5 in morulae and ...prevent spindle formation in blastocysts was ...in metaphase, or to a co-treatment of MG132 and ...of ...
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The mesh is a network of microtubule connectors that stabilizes individual kinetochore fibers of the mitotic spindle
... compared mitotic progression in cells, where GFP-TACC3 expression was induced vs not ...the metaphase plate (prometaphase-to- ...in mitotic progression are similar to those seen following depletion ...
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Schizosaccharomyces pombe Bub3 Is Dispensable for Mitotic Arrest Following Perturbed Spindle Formation
... the spindle assembly checkpoint (SAC), Mads, Bubs, and Mps1, first identified in the budding yeast, are thought to be functionally and structurally conserved through ...blocked mitotic progression when ...
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Mitotic spindle association of TACC3 requires Aurora‐A‐dependent stabilization of a cryptic α‐helix
... bipolar spindle assembly, although this process is much slower in its absence, and that the major phenotype results from defects and delays in anaphase (Hegarat et al, ...central spindle assembly (Lioutas & ...
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Dissection of the mitotic and nuclear functions of Chromator, a nuclear derived spindle matrix component in Drosophila
... at metaphase. In the absence of microtubules, the Megator spindle remains intact and shows extensive co-alignment with the Skeletor ...the spindle-like structure comprised of Megator and Skeletor ...
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Mitotic spindle association of TACC3 requires Aurora‐A‐dependent stabilization of a cryptic α‐helix
... bipolar spindle assembly, although this process is much slower in its absence, and that the major phenotype results from defects and delays in anaphase (Hegarat et al, ...central spindle assembly (Lioutas & ...
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Saccharomyces cerevisiae BUB2 Prevents Mitotic Exit in Response to Both Spindle and Kinetochore Damage
... The spindle assembly checkpoint controls the timing family of proteins that play important roles in cytokinesis of the metaphase-to-anaphase transition, inhibiting sis- and cell morphology (Field and Kellog ...
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Mitotic Spindle Form and Function
... of spindle length and stability Microtubule-based motors are the major regulators of spindle length (Saunders and Hoyt 1992; Saunders et ...Kip1, spindle length ...the spindle midzone may be ...
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Coordinated cell polarization and mitotic spindle
... of spindle orientation resulted in a similar cystogenesis defect as LGN knockdown, further supporting the notion that LGN is involved in regulating spindle orientation during ...regulating spindle ...
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INVESTIGATING MECHANISMS OF MITOTIC SPINDLE POSITIONING
... thinking to myself “that’s what I want to work on.” So, at the places I applied, I requested meetings with everyone working on C. elegans embryogenesis. Once I decided to go to graduate school at the University of North ...
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Human asparagine synthetase associates with the mitotic spindle
... the mitotic spindles and that it may have acquired a second role in mitosis similar to other metabolic enzymes that contribute to metabolic reprogramming in cancer ...
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Chromatin Structure and Differential Accessibility of Homologous Human Mitotic Metaphase Chromosomes
... The approach we have taken for studying the structure of highly condensed metaphase chromosomes is one of many applications of AFM. AFM also has broad usage in the field of nanobiosciences that merge biology-, ...
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