The Poliovirus 2C Cis-acting replication element-
Identification of a cis-Acting Replication Element within the Poliovirus Coding Region
... for 2C loop function, other domains of the structure may also con- ...with poliovirus sequences or proteins, as no second-site rever- tants were ...the 2C loop with proteins from uninfected Ohio- ...
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Mutational Disruption of cis-Acting Replication Element 2C in Coxsackievirus B3 Leads to 5′-Terminal Genomic Deletions
... transcribed RNA produces infectious CVB3 particles that can be isolated and transferred to fresh cell cultures. Inoculation of CVB3 and CVB3-CKO RNA into mice gener- ates virus. Our observations that CVB3-CKO produced ...
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Poliovirus cis-Acting Replication Element-Dependent VPg Uridylylation Lowers the Km of the Initiating Nucleoside Triphosphate for Viral RNA Replication
... A cis-acting replication element (CRE) within picornavirus RNAs serves as a template for the uridylylation of VPg, resulting in the synthesis of VPgpUpU OH ...RNA replication and that ...
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A new cis-acting element for RNA replication within the 5' noncoding region of poliovirus type 1 RNA.
... of poliovirus type 1, so that mutations occurring during the adaptation process provided the virus strain with the ability to grow normally at 40 8 C in PVR-mouse ...
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Functional Dissection of a Poliovirus cis-Acting Replication Element [PV-cre(2C)]: Analysis of Single- and Dual-cre Viral Genomes and Proteins That Bind Specifically to PV-cre RNA
... Our dual-cre viruses showed less-than-expected differences when analyzed in one-step growth curve experiments. Com- pared to the results from in vitro uridylylation assays using dual-cre P/L replicon RNAs, the ...
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A cis-Acting Element That Directs Circular Adeno-Associated Virus Replication and Packaging
... These enzymes would unambiguously distinguish between dif- ferent replicative intermediates as well as input plasmid DNA. DpnI selectively cleaves methylated input plasmid but is inac- tive against templates that have ...
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Anti-HCV RNA aptamers targeting the genomic cis-acting replication element
... HCV Replication by Selected Aptamers in Cell Culture To test the inhibitory activity of the selected molecules on HCV replication, and therefore to evaluate their therapeutic potential, Huh-7 cells that ...
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cis-acting lesions targeted to the hydrophobic domain of a poliovirus membrane protein involved in RNA replication.
... we analyzed the amino acid replacement mutations, we found that the distribution of the different mutated 3A and 3AB proteins between the membrane-bound or pellet frac- tion and the free[r] ...
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EWSR1 Binds the Hepatitis C Virus cis-Acting Replication Element and Is Required for Efficient Viral Replication
... Viral Replication Todd ...the cis-acting replication element (CRE), is located within the NS5B coding ...viral replication, the function of the interaction, and its regulation ...
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cis- and trans-Acting Elements in Flavivirus RNA Replication
... RNA replication, we used trans-complementation analysis of full-length infectious RNAs of Kunjin (KUN) virus with a range of lethal in-frame deletions in the nonstruc- tural coding region, using as helper a repBHK ...
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A cis-Acting Replication Element in the Sequence Encoding the NS5B RNA-Dependent RNA Polymerase Is Required for Hepatitis C Virus RNA Replication
... the replication of RNA ...RNA replication, implicating it as an essential cis-acting replication element ...RNA replication, and the upper helix appears to serve as an ...
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The Red Clover Necrotic Mosaic Virus RNA2 trans-Activator Is Also a cis-Acting RNA2 Replication Element
... the cis-acting sequences that are required for RNA2 replication in cowpea protoplasts, using a series of deletion mutants in the presence of ...However, replication of RNA2 was not affected by ...
Fine Mapping of a cis-Acting Sequence Element in Yellow Fever Virus RNA That Is Required for RNA Replication and Cyclization
... Although the sequences responsible for cyclization have not been identified, thermodynamic studies have shown that cy- clization results from hydrogen bonding, that the circular form is stable at 37°C under physiological ...
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cis-Acting signals that promote genome replication in rotavirus mRNA.
... the cis-acting signals that they recognize in viral ...essential replication signal of the plus-strand RNA 5 9 -UGUG ACC-3 9 at residues that site-specific mutagenesis indicates are key for the ...
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Sequence Requirements for Viral RNA Replication and VPg Uridylylation Directed by the Internal cis-Acting Replication Element (cre) of Human Rhinovirus Type 14
... the replication of HRV-14 ...for replication and which nucleotide substitutions are tolerated without significant degradation of cre ...RNA replication is closely correlated with their ability to ...
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3′-Terminal Sequence in Poliovirus Negative-Strand Templates Is the Primary cis-Acting Element Required for VPgpUpU-Primed Positive-Strand Initiation
... viral replication proteins and reduced the time the template RNA was incubated in the HeLa S10 translation-replication ...RNA replication was measured as described ...
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Stem-Loop III in the 5′ Untranslated Region Is a cis-Acting Element in Bovine Coronavirus Defective Interfering RNA Replication
... 3). In pSLIII-mut2L, 5 of the 8 bases in the left arm were FIG. 2. Integrity of stem III is required for DI RNA replication as determined by analysis of mutant 1 variants of pDrep1. The mfold-predicted structures ...
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Stem-Loop IV in the 5′ Untranslated Region Is a cis-Acting Element in Bovine Coronavirus Defective Interfering RNA Replication
... DISCUSSION In this study, we have shown by enzyme structure probing the existence of a predicted bulged stem-loop IV mapping at nt 186 through 215 and therefore lying mostly within the 210-nt 5 ⬘ UTR of BCoV DI RNA. ...
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cis-acting requirements for the replication of flock house virus RNA 2.
... The 5' ends of RNA 2 transcripts expressed from different plasmids were mapped by primer extension and compared with the 5' ends of their positive-strand replication products and authent[r] ...
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A cis-acting function for the coronavirus leader in defective interfering RNA replication.
... To test the hypothesis that the 65-nucleotide (nt) leader on subgenomic mRNAs suffices as a 5'-terininal cis-acting signal for RNA replication, a corollary to the notion that coronavirus[r] ...
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