Three-dimensional cell culture
A Bioengineered Three Dimensional Cell Culture Platform Integrated with Microfluidics To Address Antimicrobial Resistance in Tuberculosis
... medium culture, 2D culture, zebrafish, and mice ...host cell survival (22), suggesting that a fully humanized system structured in three dimensions with an extracellular matrix is needed to ...
14
A Three-Dimensional Cell Culture System To Model RNA Virus Infections at the Blood-Brain Barrier
... Human-based cell models of the BBB microvasculature include those using immor- talized cell lines (8–10) or human pluripotent stem cells that can be differentiated into microvascular endothelial cells ...
18
Embryonic stem cells in scaffold-free three-dimensional cell culture: osteogenic differentiation and bone generation
... further cell types ...complex cell-driven process of bone formation starts early in the embryo and results in bone tissue with unique features that combines stiffness and elasti- city with the ability to ...
6
A Three-Dimensional Cell Culture Model To Study Enterovirus Infection of Polarized Intestinal Epithelial Cells
... using cell lines, such as Caco-2 cells, under standard 2-D culture ...between cell culture and in vivo ...other cell types, including immune components as well as a bacterial microbiome ...
17
Squaramide-Based Supramolecular Materials for Three-Dimensional Cell Culture of Human Induced Pluripotent Stem Cells and Their Derivatives
... natural cell microenvironment; however, it is equally necessary that the gels maintain cell viability and phenotype while permitting reisolation without stress, especially for use in the stem cell fi ...
9
Three-dimensional cell culture can be regulated by vibration: low-frequency vibration increases the size of olfactory ensheathing cell spheroids
... generated. This NLM system has been tested using not only OECs, but also human neural progenitor cells (ReNcell VM cell lines), as well as primary Schwann cells and Astrocytes [23]. This system has also shown to ...
9
Three dimensional cell culture: The missing link in drug discovery
... the cell suspension and a stirring element to ensure that the cell suspension is continuously mixed ...3D cell spheroid generation and, depending on the size of the bioreactor, can enable large ...
10
Inventions and Innovations in Preclinical Platforms for Cancer Research
... three-dimensional cell culture and anticancer drug activity evaluation in a microfluidic chip. Lee, S.-A., Kang, E., Ju, J., Kim, D.-S., and Lee, S.-H., Spheroid-based three-dimensional [r] ...
26
Three-dimensional tumor cell cultures employed in virotherapy research
... tumor cell cultures to provide meaningful predictive preclinical evaluation ...models, three-dimensional tumor cell-culture systems can be employed in preclinical virotherapy ...tumor ...
15
Evaluation of the secretion and release of vascular endothelial growth factor from two-dimensional culture and three-dimensional cell spheroids formed with stem cells and osteoprecursor cells
... the cell spheroids was analyzed qualita- tively by a Live/Dead Kit assay (Molecular Probes, Eugene, USA) on day ...The cell spheroids were washed twice with PBS, followed by suspension in 1 mL of α-MEM, ...
7
A nanoporous surface is essential for glomerular podocyte differentiation in three-dimensional culture
... that cell–matrix interactions are based on both molecular and geometrical characteristics, the relationship between specific cell types and the three-dimensional morphology of the surface to ...
17
Fibroin and fibroin blended three-dimensional scaffolds for rat chondrocyte culture
... After cultivation on different types of constructs for 28 days, chondrocyte morph- ology and secretion of ECM were determined. Regardless of the types of materials used, chondrocytes found on the surface of constructs ...
12
Three Dimensional In Vitro Culture of Murine Secondary Follicles in a Defined Synthetic Matrix
... 3D culture matrix, in terms of its ability to support murine 3D follicular growth, and to determine if the new culture matrix is worth further study using human follicles in the ...the culture of ...
19
Metabolomics footprinting of three dimensional bioreactors with applications to in-cell NMR
... The primary energy source for the 2D cultures proved to also be glucose during the first three time points measured after culturing (Figure 3-5). Lactate production during this initial phase of the study was ...
153
Application of three dimensional culture conditions to human embryonic stem cell derived definitive endoderm cells enhances hepatocyte differentiation and functionality
... for cell replacement therapies or as an in vitro platform to conduct new drug ...monolayer culture may hinder the maturation of hESC-derived hepatocytes, since such two-dimensional (2D) conditions do ...
8
Characterization of mesenchymal stem cells and fibrochondrocytes in three-dimensional co-culture: analysis of cell shape, matrix production, and mechanical performance
... direct cell–cell contact is not neces- sary for phenotypic changes in MSC ...pellet culture have noted increased matrix expression in co-cultures compared to mono-culture controls, which could ...
10
Comparative analysis of ABCG2+ and ABCG2- retinoblastoma cells in three-dimensional culture
... each cell line was subjected to the same culture conditions used for embryoid body (EB) formation applied to adherent iPSC ...the cell lines were transitioned to mTeSR-1 media (Stem Cell ...
9
Three-dimensional culture and interaction of cancer cells and dendritic cells in an electrospun nano-submicron hybrid fibrous scaffold
... after culture in 3D hybrid NFS, as well as in 2D culture dish, indicating that electrospun PCL nanofibers are nonreactive with ...2D culture dish and 3D hybrid ...2D culture dish showed more ...
13
Jaagsiekte Sheep Retrovirus Transformation in Madin-Darby Canine Kidney Epithelial Cell Three-Dimensional Culture
... on cell contact with the extracellular matrix (ECM) of the Matrigel, since internal subacini also showed ...Matrigel culture and after 5 days in collagen ...3-D culture might be at- tributed to ...
12
Stem cell properties of human clonal salivary gland stem cells are enhanced by three-dimensional priming culture in nanofibrous microwells
... The culture-expanded SGSCs maintained fibroblast-like morphological consistency and constantly proliferated during ...stem cell markers, but negative for hematopoietic stem cell markers (CD45 and ...
15