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three-dimensional electron microscopy (3D-EM)

Nucleoprotein-RNA Orientation in the Measles Virus Nucleocapsid by Three-Dimensional Electron Microscopy

Nucleoprotein-RNA Orientation in the Measles Virus Nucleocapsid by Three-Dimensional Electron Microscopy

... and three-dimensional (3D) reconstruction in an iterative projection-matching procedure similar to that for IHRSR (7, 8) with the SPIDER package (9, 22), starting from a smooth helix of a chosen pitch as ...

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Three Dimensional Analysis of Melanosomes Isolated from B16 Melanoma Cells by Using Ultra High Voltage Electron Microscopy

Three Dimensional Analysis of Melanosomes Isolated from B16 Melanoma Cells by Using Ultra High Voltage Electron Microscopy

... Free electron laser photoemission electron microscopy data is attributed to eumelanin on the surface of human iridal stroma melanosomes [1]. The surface of the iridal stroma melanosomes is comprised ...

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Reduced-dose and high-speed acquisition strategies for multi-dimensional electron microscopy

Reduced-dose and high-speed acquisition strategies for multi-dimensional electron microscopy

... the three-dimensional (3D) characterisation of nanoma- terials, frequently playing a unique role in elucidating nanostructure-property relationships ...transmission electron microscopy (TEM) ...

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Nonenzymatic glycosylation induced modifications of intact bovine kidney tubular basement membrane

Nonenzymatic glycosylation induced modifications of intact bovine kidney tubular basement membrane

... Morphological analysis using high resolution, low voltage scanning electron microscopy (LV-SEM) revealed a complex three-dimensional meshwork of interconnecting strands with intervening ...

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Visualization of HIV T Cell Virological Synapses and Virus-Containing Compartments by Three-Dimensional Correlative Light and Electron Microscopy

Visualization of HIV T Cell Virological Synapses and Virus-Containing Compartments by Three-Dimensional Correlative Light and Electron Microscopy

... CLEM reveals structures associated with T cell virological synapses. To examine the structural organization of T cell-T cell interactions at virological synapses and the nature of the virus-containing compartment, we ...

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Sequence and Structural Characterization of Great Salt Lake Bacteriophage CW02, a Member of the T7-Like Supergroup

Sequence and Structural Characterization of Great Salt Lake Bacteriophage CW02, a Member of the T7-Like Supergroup

... cryogenic electron microscopy and three-dimensional reconstruction, enabled the fitting of a portion of the bacteriophage HK97 capsid protein into CW02 capsid density, thereby providing ...

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In-situ Scanning Electron Microscopy for Electron-beam Lithography and In-situ One Dimensional Nano Materials Characterization

In-situ Scanning Electron Microscopy for Electron-beam Lithography and In-situ One Dimensional Nano Materials Characterization

... by electron gun bombardment 36 . To demonstrate this approach, three consecutive I-V characterization were performed on the sample shown in figure 19 ...

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Electron microscopy of quantum dots

Electron microscopy of quantum dots

... Secondary electron imaging in a scanning electron micro- scope exhibits strong topographic contrast and can thus be used to measure size distributions of quantum dots on exposed surfaces if a field-emission ...

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THREE-DIMENSIONAL OBSERVATIONS ON THICK BIOLOGICAL SPECIMENS BY HIGH VOLTAGE ELECTRON MICROSCOPY

THREE-DIMENSIONAL OBSERVATIONS ON THICK BIOLOGICAL SPECIMENS BY HIGH VOLTAGE ELECTRON MICROSCOPY

... After the histochemical reactions all the specimens were coated with thin layer of carbon and were observed in JEOL JEM-4000EX or Hitachi H-1250M high voltage electron microscopes at accerelating voltages of 400 ...

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Electron microscopy of bacteriophage phi 6 nucleocapsid: three-dimensional image analysis.

Electron microscopy of bacteriophage phi 6 nucleocapsid: three-dimensional image analysis.

... 3 provided direct proof that the three distinct forms seen in the electron microscope after negative staining with PTA at pH 7 were merely different views of the same NC In the preceding[r] ...

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Cryo electron Microscopy Structures of Chimeric Hemagglutinin Displayed on a Universal Influenza Vaccine Candidate

Cryo electron Microscopy Structures of Chimeric Hemagglutinin Displayed on a Universal Influenza Vaccine Candidate

... no three-dimensional (3D) structural infor- mation is available on these recombinant HA proteins either in native form or when they are bound to neutralizing ...

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Tungsten oxide-Au nanosized film composites for glucose oxidation and sensing in neutral medium

Tungsten oxide-Au nanosized film composites for glucose oxidation and sensing in neutral medium

... scanning electron microscopy (FESEM), X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), micro- Raman spectroscopy, and transmission electron microscopy ...

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Three-dimensional super-resolution microscopy of the inactive X chromosome territory reveals a collapse of its active nuclear compartment harboring distinct Xist RNA foci

Three-dimensional super-resolution microscopy of the inactive X chromosome territory reveals a collapse of its active nuclear compartment harboring distinct Xist RNA foci

... cence microscopy have described a compacted sphere-like Xi/Barr body in contrast to a flat and extended active X (Xa) territory ...In three-dimensional (3D) reconstructions from ultrathin TEM serial ...

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On the three-dimensional configuration of electrostatic solitary waves

On the three-dimensional configuration of electrostatic solitary waves

... Above we have considered possible algorithms to con- struct the simplest quasi-one-dimensional models of EH without invoking the boundary conditions typical of a lab- oratory plasma bounded by a waveguide. ...

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Improved osseointegration properties of hierarchical microtopographic/nanotopographic coatings fabricated on titanium implants

Improved osseointegration properties of hierarchical microtopographic/nanotopographic coatings fabricated on titanium implants

... force microscopy (AFM) data, the polished surface of the Ti substrate after mechanical treatment has been characterized by low rough- ness: medium height (Ra) was ~ ...

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High spatial resolution mapping of surface plasmon resonance modes in single and aggregated gold nanoparticles assembled on DNA strands

High spatial resolution mapping of surface plasmon resonance modes in single and aggregated gold nanoparticles assembled on DNA strands

... The field of plasmonics has become a topic of major interest in the last years due to its property of showing an enhancement of the electromagnetic field at a sub- wavelength dimension [1]. This phenomenon is especially ...

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Production and Characterisation of Reticulated Glass Reinforced Hydroxyapatite Foams for Hard Tissue Surgery

Production and Characterisation of Reticulated Glass Reinforced Hydroxyapatite Foams for Hard Tissue Surgery

... tested three deflocculants, Jargon, Dolapix and Reotan on alumina suspensions, out of these they found Jargon to be the most effective with an optimal addition of ...

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Three dimensional confocal microscopy of fluorescent microspheres: Imaging and size determination

Three dimensional confocal microscopy of fluorescent microspheres: Imaging and size determination

... The bright center in fig. 3(b) can be explained in terms of geometrical optics: when the light is focused on the center of the sphere, rays propagate along radial directions and reflect back. In order to see the rings ...

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Measurement and Evaluation of Local Surface Temperature Induced by Irradiation of Nanoscaled or Microscaled Electron Beams

Measurement and Evaluation of Local Surface Temperature Induced by Irradiation of Nanoscaled or Microscaled Electron Beams

... 25 kV, respectively, with increasing beam currents. The beam spot diameter was fixed at 1 μm and the measure- ment time was fixed at 30 s. Calibration experiments re- vealed that the average sensitivity of Pt-Cr TFTCs ...

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Cryo electron microscopy to determine the structure of macromolecular complexes

Cryo electron microscopy to determine the structure of macromolecular complexes

... lens. Electron lenses have spherical aberration, and defocussing introduces differences between the scattered and unscattered rays that result in visible ...

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