Topoisomerase II Alpha
Maternal topoisomerase II alpha, not topoisomerase II beta, enables embryonic development of zebrafish top2a / mutants
... top2a mutants exhibit defects in cell cycle progression Topoisomerase II alpha genes are known to be critical for cell cycle progression. Therefore, we quantified, by flow cytometry, the distribution ...
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<p>3,6-Disubstituted 1,2,4-Triazolo[3,4-<em>b</em>]Thiadiazoles with Anticancer Activity Targeting Topoisomerase II Alpha</p>
... Materials and Methods: The MTT assay was performed in DLD-1, HT-29, and LoVo cancer cells so as to evaluate the antiproliferative activity of KA25, KA26, and KA39 triazolo[3,4-b]thiadiazole derivatives. The KA39 compound ...
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Topoisomerase II alpha and TLE3 as predictive markers of response to anthracycline and taxane-containing regimens for neoadjuvant chemotherapy in breast cancer
... Nonetheless, considering that this was the first study that investigated the role of concomitant evaluation of TOP2A and TLE3 as markers of response to neoadjuvant chemo- therapy, furt[r] ...
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Topoisomerase II alpha gene amplification is a favorable prognostic factor in patients with HER2 positive metastatic breast cancer treated with trastuzumab
... Abbreviations CEN17: Centromere 17; CI: Confidence interval; ER: Estrogen receptor; FFPE: Formalin-fixed paraffin-embedded; HER2: Human epidermal growth factor receptor 2; HR: Hazard rat[r] ...
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The significance of Epstein Barr Virus (EBV) & DNA Topoisomerase II alpha (DNA-Topo II alpha) immunoreactivity in normal oral mucosa, Oral Epithelial Dysplasia (OED) and Oral Squamous Cell Carcinoma (OSCC)
... anti-human Topoisomerase II α protein (DAKO, clone: Ki-S1, isotype: IgG2a) were ...Topo II α antibody provided in liquid form as purified IgG diluted in ...
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P53, MAPK, topoisomerase II alpha and Ki67 immunohistochemical expression and KRAS/BRAF mutation in ovarian serous carcinomas
... topoII alpha immunoexpression, are more likely to follow the type II pathway and these markers could be a useful additional tool in distinguishing the low- and high- grade groups of OSCs, along with nuclear ...
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Protein interactions at the human topoisomerase II[alpha] promoter : a thesis presented to Massey University in partial fulfilment of the requirement for the degree Doctor of Philosophy in Biochemistry
... the topoisomerase IIα ...for topoisomerase IIα expression can only be speculated by comparison with other ...the topoisomerase IIα promoter in vivo, ChIP assays could be carried out using antibodies ...
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QSAR and docking studies on xanthone derivatives for anticancer activity targeting DNA topoisomerase IIα
... cates the prediction accuracy of the model, was 0.82. The QSAR study indicates that dielectric energy, group count (hydroxyl), LogP, shape index basic (order 3), and solvent- accessible surface area were significantly ...
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A role for topoisomerase II alpha in chromosome damage in human cell lines
... topo II activity in HL60 and MX2 cells with the kinetoplast DNA decatenation assay and found that topo II purified from nuclear extracts was on average four times (75%) less active in MX2 cells when ...
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Analysis in Carcinoma Stomach Her-2/neu Positivity by Immuno-histochemistry
... 40 Tanner M, Hollmen M, Junttila TT, et al: Amplification of HER-2 in gastric carcinoma: association with topoisomerase II alpha gene amplification, intestinal type, poor prognosis and s[r] ...
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Comparative evaluation of three proliferation markers, Ki-67, TOP2A, and RacGAP1, in bronchopulmonary neuroendocrine neoplasms: Issues and prospects
... Ki-67, Topoisomerase II alpha (TOP2A), and RacGAP1, in a series of tumor samples from 104 BP-NEN patients (24 TC, 21 AC, 52 SCLC, 7 LCNEC) using different evaluation methods (immunohistochemistry ...
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Hypersensitive Inhibition of the Proliferation of Cells with Mutated DNA Repair Related Genes by the Catalytic Topoisomerase II Inhibitor 20 O IngenolEZ
... exhibit topoisomerase inhibitory ...both topoisomerase II (topo II) activity and cell proliferative ...activity. Topoisomerase II inhibitors can be divided into the poison and ...
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The ATP dependent chromatin remodelling enzyme Uls1 prevents Topoisomerase II poisoning
... Deletion of ULS1 does not result in a dramatic growth de- fect or in sensitivity to a variety of DNA damaging drugs (Supplementary Figure S1A). This apparent absence of phenotype initially hindered our attempts to ...
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In silico identification and evaluation of hits for the inhibition of topoisomerase-ii enzyme via structure based pharmacophore mapping and virtual screening
... Human topoisomerase II beta in complex with DNA and etoposide (PDB codes: 3QX3) ...of topoisomerase II beta (PDB ID: ...of topoisomerase II as reference ...
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Contribution of Immunohistochemistry in Prognostic Assessment of Epithelial Ovarian Carcinoma – Review of the Literature I.
... and topoisomerase II α are most frequently evaluated immunohistochemically detectable proliferation ...Type II DNA topoisomerases are nuclear enzymes that play a crucial role in DNA ...rase II ...
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cGAS/STING axis mediates a topoisomerase II inhibitor–induced tumor immunogenicity
... Top II inhibitors induced IFN-I pathway activation through the ATM and cGAS/STING pathway, which can prevent the Ebola virus infection ...Top II inhibitor, elicited NF-κB activation in a STING-de- pendent ...
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Genetic analysis of the gyrase A-like domain of DNA topoisomerase II of Saccharomyces cerevisiae.
... used tetrad analysis to group the extragenic suppres- sor mutations. Tetrad analysis showed that seven of the 16 tos mutations fell into one linkage group, which we refer to[r] ...
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SIZ1/SIZ2 Control of Chromosome Transmission Fidelity Is Mediated by the Sumoylation of Topoisomerase II
... The results showing that the simultaneous loss of Siz1p and Siz2p activity is epistatic to the top2 SNM al- leles and yields an unmodified Top2p suggest that the role of SUMO E3 in chromosome segregation could be limited ...
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Assessment of drug induced genotoxicity in mammalian cells and the contribution of topoisomerase II inhibition
... groups. Topoisomerase I enzymes cleave a single strand of DNA, thus allowing the complimentary strand to pass through the ...nick. Topoisomerase II enzymes cleave both DNA strands, thus allowing ...
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A panel of glycoproteins as candidate biomarkers for early diagnosis and treatment evaluation of B-cell acute lymphoblastic leukemia
... rich alpha - 2 - glycoprotein 1; CLU: Clusterin; F2: thrombin; SERPIND1: heparin cofactor II; A2M: alpha-2- macroglobulin; SERPINF2: alpha-2-antiplasmin; SERPINA1: Alpha-1 antitrypsin; ...
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