total DNA
An estimate of the total DNA in the biosphere
... The total information content of prokaryotes was found from the product of the mean ge- nome size of prokaryotes and estimates of the total number of prokaryotes on the earth, giving a value of ...the ...
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Colorimetric detection of both total genomic and loci-specific DNA methylation from limited DNA inputs
... Total DNA methylation is traditionally detected by HPLC [22] and mass spectrometry (MS) [23] methods. While our MBD assay uses less starting material, it is difficult to directly compare the ...
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DNA Packaging Mutant: Repression of the Vaccinia Virus A32 Gene Results in Noninfectious, DNA-Deficient, Spherical, Enveloped Particles
... viral DNA. The synthesis of viral DNA was determined by blotting of restriction digests of total DNA from cells infected with wild-type or mutant virus in the presence or absence of IPTG and ...
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CHLOROPLAST DNA VARIATION IN PEARL MILLET AND RELATED SPECIES
... Comparison of autoradiographs prepared from total DNA digests and from cpDNA digests verified that the fragments homologous to each cloned DNA sequence employed in th[r] ...
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Typing of Enterococcus species by DNA restriction fragment analysis
... Total DNA was digested with a suitable restriction enzyme SstI, and the pattern of bands produced after electrophoresis and staining was used for comparison of isolates.. Secondly, DNA d[r] ...
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Direct Evidence of Lower Viral Replication Rates In Vivo in Human Immunodeficiency Virus Type 2 (HIV-2) Infection than in HIV-1 Infection
... age, total viral DNA, integrated proviral DNA, viral mRNA, plasma viral load, and percentage of total viral DNA integrated between HIV-1- and HIV-2-infected subjects; two-sided P values ...
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Use of the checkerboard DNA DNA hybridization technique for bacteria detection in Aedes aegypti (Diptera:Culicidae) (L )
... checkerboard DNA-DNA hybridization technique [5-8] is a semi-quantitative technique that has been extensively employed in odontology to detect and quan- tify a variety of bacterial species in dental samples ...
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Nucleic Acid Sequence Based Amplification Assay for Human Papillomavirus mRNA Detection and Typing: Evidence for DNA Amplification
... amplifiable DNA and to assess the effectiveness of the DNase treatments, the NA extracts were assayed by real-time PCR for the -globin gene (forward primer, 5⬘-CAG GTA CGG CTG TCA TCA CTT AGA-3⬘; reverse primer, ...
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Using CF11 cellulose columns to inexpensively and effectively remove human DNA from Plasmodium falciparum infected whole blood samples
... human DNA contamination but still met the threshold level for sequencing, indicating that some variation in column preparation can be ...human DNA depletion or total DNA recovery when filtered ...
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Total HIV DNA: a global marker of HIV persistence
... of total DNA is limited per PCR well, it is often necessary to test several replicates, in order to increase the number of cells tested and to estimate the frequency of infected cells, as well as possible ...
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Novel Assays for Measurement of Total Cell Associated HIV 1 DNA and RNA
... HIV-1 DNA and RNA, targeting a highly conserved region in HIV-1 pol, with sensitivities of 3 to 5 copies/1 million ...ART. Total and resting CD4 ⴙ T cells were isolated from a subset of patients and tested ...
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A New Histone Structure Which Binds DNA at Its Eight Subunit N Termini
... the DNA is ...the DNA is therefore placed there. The role models for the protein and DNA structures in the N-terminal domain are taken from the comparable role models for protein and DNA in ...
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Detection of low frequency <em>FGFR3</em> mutations in the urine of bladder cancer patients using next-generation deep sequencing
... urine DNA sample was assayed for FGFR3 mutations in exons 7, 10, or ...mutant DNA (Table 5) that were present at ,1% and as low as 0.02% of the total urine ...
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Detection of anti DNA antibody using synthetic antigens Characterization and clinical significance of binding of poly (deoxyadenylate deoxythymidylate) by serum
... of DNA used to detect antibody to native DNA (nDNA) by binding assays have been found to be subtly contaminated by single stranded ...recent DNA binding data have directly challenged the unique role ...
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Differentiation of Campylobacter jejuni and Campylobacter coli strains by using restriction endonuclease DNA profiles and DNA fragment polymorphisms
... The RE profiles produced by ClaI with the genomic DNAs of all 169 Campylobacter strains were examined. The RE pro- files of selected strains of C. jejuni and C. coli from humans and chickens are shown in Fig. 2a, ...
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Role of Simian Virus 40 Vp1 Cysteines in Virion Infectivity
... remaining DNA was purified as described earlier and linearized with KpnI before being analyzed by Southern ...viral DNA was protected from DNase I ...viral DNA into virion particles, an aliquot of ...
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Identification of a CpG Island Methylator Phenotype that Defines a Distinct Subgroup of Glioma.pdf
... In the non-TCGA independent validation set examined in this study, an IDH1 mutation was detected in 40/43 (93%) low- and intermediate-grade gliomas, but only 7/57 (13%) of primary GBMs. Similarly, we detected nearly ...
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One-night sleep deprivation induces changes in the DNA methylation and serum activity indices of stearoyl-CoA desaturase in young healthy men
... Methods: Sixteen young, normal-weight, healthy men completed two study sessions, one with one-night TSD and one with one-night normal sleep (NS). Sleep quality and length was assessed by polysomnography, and consisted of ...
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Colinearity of RNAs with the vaccinia virus genome: anomalies with two complementary early and late RNAs result from a small deletion or rearrangement within the inverted terminal repetition.
... To locate the precise position of the deletion or rearrangement, EcoRI-digested total vaccinia virus DNA was hybridized to the 3' end-labeled left XhoI subfragment of the cloned DNA seg-[r] ...
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Hybridization of Rous Sarcoma Virus Deoxyribonucleic Acid Polymerase Product and Ribonucleic Acids from Chicken and Rat Cells Infected with Rous Sarcoma Virus
... Preparation of these fractions was accomplished by annealing the total B77 virus DNA with B77 virion RNA and separating hybridized "minus" from nonhybridized "plus" DNA in Cs2SO4 equilib[r] ...
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