Translation elongation
MicroRNA-33a-5p Modulates Japanese Encephalitis Virus Replication by Targeting Eukaryotic Translation Elongation Factor 1A1
... Japanese encephalitis virus (JEV) is a typical mosquito-borne flavivirus responsible for acute encephalitis and meningitis in hu- mans. However, the molecular mechanism for JEV pathogenesis is still unclear. MicroRNAs ...
13
Binding of the eukaryotic translation elongation factor 1A with the 5’UTR of HIV 1 genomic RNA is important for reverse transcription
... Background: The cellular protein eukaryotic translation elongation factor 1A (eEF1A) binds to aminoacylated transfer RNAs and delivers them to the ribosome during translation. eEF1A also binds to RNA ...
10
Overexpression of Translation Elongation Factor 1A Affects the Organization and Function of the Actin Cytoskeleton in Yeast
... The translation elongation factor 1 complex (eEF1) plays a central role in protein synthesis, delivering aminoacyl-tRNAs to the elongating ribosome. The eEF1A subunit, a classic G-protein, also performs ...
12
Purification of Components of the Translation Elongation Factor Complex of Plasmodium falciparum by Tandem Affinity Purification
... The strategy described herein provides a better way to study protein networks in the parasite and at any given time during any stage of the parasite life cycle. We have applied this strat- egy to the purification of the ...
8
Cloning, expression and functional study of translation elongation factor 2 (EF 2) in zebrafish
... Translation elongation factor 2, EF-2 and its eubacterial ho- molog, EF-G, act sequentially after eEF1a/EF-Tu to catalyze the GTP hydrolysis-dependent translocation of the ribosome during protein synthesis, ...
5
Unique Classes of Mutations in the Saccharomyces cerevisiae G-Protein Translation Elongation Factor 1A Suppress the Requirement for Guanine Nucleotide Exchange
... exchange. In addition, a K205A mutation causes lethal- ity (A ndersen et al. 2001). While eEF1Ba is normally essential for viability (H iraga et al. 1993), cells can sur- vive without the protein in the presence of ...
13
Increased expression of Saccharomyces cerevisiae translation elongation factor 1 alpha bypasses the lethality of a TEF5 null allele encoding elongation factor 1 beta.
... The strains with tefs::TRPl suppressed by extra copies of TEF2 were slow growing, cold sensitive, hypersensitive to inhibitors of translation elongation and showed i[r] ...
9
A Toxoplasma Prolyl Hydroxylase Mediates Oxygen Stress Responses by Regulating Translation Elongation
... cues impacts mRNA translation rates. Second, the abundances of individual proteins were differentially affected by stress: MIC2, SAG1, and eEF2 were downregulated while eIF2 ␣ and histone H3 were unaffected. These ...
17
A New Biophysical Model for Translation Elongation to Predict and Optimize Heterologous Protein Production in Escherichia coli.
... uses translation to synthesize proteins that are not normally expressed in production ...poor translation, non-optimal ribosome binding sites (RBS), RBS and start codon spacing, frameshifting, premature ...
280
Structural Basis for EarP Mediated Arginine Glycosylation of Translation Elongation Factor EF P
... proline-specific translation elongation factors EF-P and IF-5A is usually achieved by posttranslational elongation of the -amino group of a conserved lysine (20–23, 48, ...
20
Translation elongation factor-1 alpha interacts with the 3' stem-loop region of West Nile virus genomic RNA.
... The conserved 3 * -terminal stem-loop (3 * SL) of the West Nile virus (WNV) genomic RNA was previously used to probe for cellular proteins that may be involved in flavivirus replication and three cellular proteins were ...
12
The eukaryotic translation elongation factor 1A regulation of actin stress fibers is important for infectious RSV production
... eukaryotic translation elongation factor 1A (eEF1A) is an actin binding protein that plays a role in the formation of filamentous actin (F-actin) ...
10
Ubiquitin specific peptidase 5 regulates colorectal cancer cell growth by stabilizing Tu translation elongation factor
... contain translation-stalled mRNAs and RNA-binding proteins, presumably modulating mRNA translation to accomodate stress responses ...As translation elongation factors from bacteria, ...
13
The Nucleocapsid Protein of Severe Acute Respiratory Syndrome Coronavirus Inhibits Cell Cytokinesis and Proliferation by Interacting with Translation Elongation Factor 1α
... human elongation factor 1-alpha (EF1 ␣ ), an essential component of the translational machinery with an important role in cytokinesis, promoting the bundling of filamentous actin ...protein translation and ...
10
Translation Elongation Factor 1-Alpha Interacts Specifically with the Human Immunodeficiency Virus Type 1 Gag Polyprotein
... rified from bacterial lysate by affinity chromatography. As a control, GST was prepared in the same way, as well as GST-MA dB5, which contains a deletion encompassing the basic region of MA (Fig. 3A). The purity of the ...
14
Identification Of Protein Profile Of A. Fumigatus For Diagnostic Intrest From Clinical Isolates
... the translation elongation factor eEF. The translation process functions by a series of highly regulated steps that are catalyzed by the eukaryotic initiation ...
6
A New Look at Codon Usage and Protein Expression
... Synonymous codons are triplets of RNA nucleotides that encode the same amino acid. Sub- stitutions of one synonymous codon for another are not always silent. For instance, multiple groups have shown that rare (i.e., ...
9
Detection of the entomopathogenic fungus Beauveria bassiana in the rhizosphere of wound-stressed Zea mays plants
... sequences of the translation elongation factor 1-alpha gene (ef1 α ) were obtained in silico using and including B. bassiana (GenBank accession: AY531924.1) as a reference query. These included B. ...
16
Overexpression of human virus surface glycoprotein precursors induces cytosolic unfolded protein response in Saccharomyces cerevisiae
... eukaryotic translation elongation factor 1A (eEF1A, encoded by two identical yeast genes TEF1 and TEF2, also known as Tef2p) is localized in cell cytoplasm and has several important functions including ...
18
Morphological and phylogenic analysis of Fusarium species associated with vertical system of Orobanche spp.
... In this study, in addition to the morphological identification, the sequence data analysis of the tef1-α region was employed. The molecular data and phylogenetic relationships allow reliable differentiation between the ...
9