Transmembrane Region
A Substitution in the Transmembrane Region of the Glycoprotein Leads to an Unstable Attenuation of Machupo Virus
... Challenge of IFN- ␣ / ␥ R ⫺/⫺ mice with rMACV resulted in a biphasic disease, clinical signs, and MTD similar to what our lab- oratory reported previously when characterizing the model (36). Of the seven animals ...
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Evaluation of the contribution of the transmembrane region to the ectodomain conformation of the human immunodeficiency virus (HIV 1) envelope glycoprotein
... gp41 transmembrane Env subunits [1 – ...a transmembrane region, and trimerizes [4 – ...gp41 transmembrane region in the viral membrane, six-helix bundle formation approximates and fuses ...
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The Measles Virus Fusion Protein Transmembrane Region Modulates Availability of an Active Glycoprotein Complex and Fusion Efficiency
... its transmembrane (TM) region. Since this region influences function of the envelope proteins of other viruses, we examined its role in the MV F ...TM region leucine (L507A) was more fusogenic ...
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Multiple C2 domains and Transmembrane region Proteins (MCTPs) tether membranes at plasmodesmata
... This work was supported by the National Agency for Research (Grant ANR- 14 -CE 19 - 0006 - 01 to E.M.B), “ Osez l ’ interdisciplinarité ” OSEZ- 2017 -BRIDGING CNRS programme to E.M.B., the European Research Council (ERC) ...
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Changes in the transmembrane region of the human immunodeficiency virus type 1 gp41 envelope glycoprotein affect membrane fusion.
... The presence of wild-type levels of gpl20 glycoprotein in the supernatants of COS-1 cells transfected with the plasmids expressing the mutant envelope glycoproteins suggested that these [r] ...
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Molecular basis of Fce receptor : IgE interactions
... The FceRI-y subunit is an integral membrane protein with an extracellular region of five amino acids, a transmembrane region of 21 amino acids (as does FceRI-a), and a cytoplasmic regi[r] ...
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The Nef-Like Effect of Murine Leukemia Virus Glycosylated Gag on HIV-1 Infectivity Is Mediated by Its Cytoplasmic Domain and Depends on the AP-2 Adaptor Complex
... the transmembrane domain of M-MLV glycoGag is required for activity, we generated the gCD/AR fusion protein illustrated in ...the transmembrane and extracellular domains are both from HA-tagged ...the ...
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COMPUTATIONAL STRUCTURAL AND FUNCTIONAL ANALYSIS OF SHORT NEUROPEPTIDE F FROM TRIBOLIUM CASTANEUM
... 9 GOR analysis of short neuropeptide F from Tribolium castenium This amino acid sequence is of a MEMBRANE PROTEIN which have 1 transmembrane helix.. transmembrane region AMKCLCAVTCIMIVVA[r] ...
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Constitutive activation of a variant of the env-mpl oncogene product by disulfide-linked homodimerization.
... the transmembrane region, gener- ated a 220-nt fragment, indicating that the deletion occurred in the extracellular-domain-encoding region of the env-mpl gene (the same primers allow the ...
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Preliminary investigation of the C terminal mutations that cause malignant hyperthermia : a thesis presented to Massey University in partial fulfilment of the requirements for the degree of Master of Science in Biochemistry
... The transmembrane region of the C-terminal domain is almost entirely composed of regulator binding sites and contains the recently identified novel mutations... Sites on the N-terminal r[r] ...
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Identification by virtual screening and functional characterisation of novel positive and negative allosteric modulators of the α7 nicotinic acetylcholine receptor
... signi fi cantly different from control responses (99.9 ± 4.3% n ¼ 4, P ¼ 0.980; 102 ± 5.6% n ¼ 6, P ¼ 0.712; and 99.2 ± 1.2% n ¼ 6, P ¼ 0.568, respectively). These changes in response were signi fi - cantly different in ...
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Bacterial calpains and the evolution of the calpain (C2) family of peptidases
... Coloured dots indicate classification, additional domains and non-peptidase homologues, where brown is Bacteria, green is a signal peptide, pink is a transmembrane region, red is a CIII [r] ...
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Structure and Assembly of the Sindbis Virus E1 and E2 Transmembrane Proteins
... E2 transmembrane domain to investigate the interaction of the membrane-spanning domain with the membrane bilayer (Hernandez et ...the transmembrane domain resulted in a four-log loss of infectious virus ...
102
Molecular cloning and expression of a murine homolog of the human poliovirus receptor gene.
... A murine cDNA probe containing sequences encoding extracellular domains 2 and 3, the transmembrane region, and the NH2terminal half of the cytoplasmic tail hybridized with 2- and 3-kb mR[r] ...
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Computer-assisted analysis of envelope protein sequences of seven human immunodeficiency virus isolates: prediction of antigenic epitopes in conserved and variable regions.
... The second epitope amino acids 722 to 745 is located directly after a stretch of hydrophobic amino acids which is likely to be a transmembrane region of gp4l TM3; these hydrophilic, flex[r] ...
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Computational Predictions of G Protein Coupled Receptor Structures and Binding Sites
... (i.e. transmembrane region, large structured N-terminus, and peptide ...the transmembrane bundle structure of GLP1R bound to the peptide Exendin-4 (Exe4) (a GLP1R agonist on the market for treating ...
122
The human transmembrane proteome
... human transmembrane proteomes published so far with the data in our HTP data- base ...α-helical transmembrane proteome the Sali lab used a simple algo- rithm to create an initial set for their purpose ...
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The Structure of Barmah Forest Virus as Revealed by Cryo-Electron Microscopy at a 6-Angstrom Resolution Has Detailed Transmembrane Protein Architecture and Interactions
... alphavirus transmembrane region are available, the BFV E1/E2 transmembrane regions were modeled as ␣ -he- lices by MODELLER (6) based on secondary-structure pre- diction results from the HNN Web ...
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The M2 Ectodomain Is Important for Its Incorporation into Influenza A Virions
... and transmembrane protein interactions serving im- portant functions in assembly, as is the case for alphaviruses ...cytoplasmic region of the transmem- brane spike protein is thought to drive budding ...
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Structure-Function Studies of the Self-Assembly Domain of the Human Immunodeficiency Virus Type 1 Transmembrane Protein gp41
... FIG. 1. Diagram of the gp41 self-assembly domain. (A) Linear representation of domains in gp41. FP, fusion peptide; N, heptad repeat motif located near the fusion peptide, containing sequences from DP-107 and N36; C, ...
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