trypsin cleavage
Trypsin Cleavage Stabilizes the Rotavirus VP4 Spike
... Proteolytic cleavage of VP4 enhances viral infectivity severalfold (1, ...on trypsin cleavage of VP4 at amino acid 247 ...for trypsin-enhanced infectivity of rotaviruses, we examined the SA11 ...
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Antibodies to the trypsin cleavage peptide VP8 neutralize rotavirus by inhibiting binding of virions to target cells in culture.
... MAbs directed at VP8*, the smaller trypsin cleavage fragment of VP4, were shown to efficiently prevent binding of radiolabeled virions to MA104 cell monolayers, to an extent and at conce[r] ...
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Location of intrachain disulfide bonds in the VP5* and VP8* trypsin cleavage fragments of the rhesus rotavirus spike protein VP4.
... Electrophoretic analysis of VP5d774 under reducing and nonreducing conditions showed that the protein retained the capacity to form an intrachain disulfide bond and that the nonreduced f[r] ...
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A comparison of the folding characteristics of free and ribosome-tethered polypeptide chains using limited proteolysis and mass spectrometry.
... the trypsin cleavage rate is different for the free and ribosome-bound nascent chains, with less extensive digestion detected in the presence of the ...
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Trypsin activation pathway of rotavirus infectivity.
... by trypsin, the VP4 protein is cleaved at three conserved arginines, located at positions 231, 241, and 247, extending our previous findings that SA114fM VP4 was cleaved at arginines 241 and 247 and identifying ...
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Presence of a Surface-Exposed Loop Facilitates Trypsinization of Particles of Sinsiro Virus, a Genogroup II.3 Norovirus
... of trypsin in serial twofold dilutions, beginning with the highest concentration of 500 g/ml, for 30 min at ...predominant cleavage products were observed on SDS-PAGE ...the trypsin- treated VLPs ...
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Identification of an Epitope Common to Genogroup 1 “Norwalk Like Viruses”
... In conclusion, MAbs NV2461, NV3901, and NV3912 appear to map to a single epitope that is common to G1 NLVs. This common epitope is present in monomeric forms of the 32K trypsin cleavage product and has been ...
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A Metastable Form of the Large Envelope Protein of Duck Hepatitis B Virus: Low-pH Release Results in a Transition to a Hydrophobic, Potentially Fusogenic Conformation
... novel trypsin cleavage site normally hidden from the particle surface, generating a prod- uct of 25 kDa, comprised of the entire DHBV pre-S domain plus the first transmembrane region within the S domain ...
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Specific proteolytic cleavage of recombinant Norwalk virus capsid protein.
... specific cleavage of rNV cap- sid protein. Because specific proteolytic cleavage of a viral capsid protein is a common property in the life cycles of other enteric viruses, we investigated whether a ...
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Proteolytic Activation of the Porcine Epidemic Diarrhea Coronavirus Spike Fusion Protein by Trypsin in Cell Culture
... of trypsin, which correlated with a clear proteolysis of S proteins on ...virions. Trypsin cleavage of virion-bound Sca yielded a dominant 70-kDa-sized S2 fragment with an intact C terminus ...with ...
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A Single Point Mutation Creating a Furin Cleavage Site in the Spike Protein Renders Porcine Epidemic Diarrhea Coronavirus Trypsin Independent for Cell Entry and Fusion
... protease, trypsin, in the cell culture medium to activate the S protein for membrane fusion and for virus propagation in cell culture ...This trypsin dependency in vitro is consistent with the tissue ...
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Embryo signals for successful implantation
... first time sequence recorded baseline activity for 2 minutes, 10ul of embryo conditioned medium was applied (to give a 1/20 dilution) and images were recorded for the following 5 minutes. After a 10 minute interval the ...
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Cleavage and Activation of the Severe Acute Respiratory Syndrome Coronavirus Spike Protein by Human Airway Trypsin-Like Protease
... (A) Cleavage of SARS-S by HAT in cotransfected 293T ...with trypsin or PBS. Subsequently, S-protein cleavage was detected by Western blot analysis of cell lysates using a serum specific for the S1 ...
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Comparative analysis of trypsin inhibitor activity in common pulses and its partial purification
... the Trypsin enzyme to check the activity of Trypsin ...the Trypsin inhibitor activity and the molecular weight of Trypsin inhibitor by SDS PAGE ....The Trypsin inhibitor is the enzyme ...
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Trypsin Inhibitors of Human Serum I Standardization Mechanism of Reaction, and Normal Values
... EFFECT OF CALCIUM ON THE INHIBITION OF TRYPSIN BY SOYBEAN INHIBITOR Residual trypsin as a function of increasing amounts of soybean inhibitor for two different trypsin concentrations: 0-[r] ...
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Effects of antibodies, trypsin, and trypsin inhibitors on susceptibility of neonates to rotavirus infection
... Breast-fed infants who did not excrete rotavirus over the 5-day period received milk of significantly higher antirotaviral secretory immunoglobulin A or trypsininhibitory capacity or bot[r] ...
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Genomic analysis of the tryptome reveals molecular mechanisms of gland cell evolution
... One trypsin (NVJ_127465) was not reported in the single-cell dataset [16] but was among the genes found to be significantly upregulated in the tissue-specific tran- scriptome of nematosomes, which may also play a ...
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VP2 Cleavage and the Leucine Ring at the Base of the Fivefold Cylinder Control pH-Dependent Externalization of both the VP1 N Terminus and the Genome of Minute Virus of Mice
... VP2 cleavage proved unsuitable for anal- ysis of the L172T mutant because it was not possible to purify homogeneous L172T populations following tryptic ...to cleavage with ...to trypsin at 37°C, ...
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Modification of the Trypsin-Dependent Cleavage Activation Site of the Human Metapneumovirus Fusion Protein To Be Trypsin Independent Does Not Increase Replication or Spread in Rodents or Nonhuman Primates
... containing trypsin to preclude selective pressure for ...F cleavage site mutants in the ⌬ SH/G ...F cleavage site was manipulated. (B) Sequences of the F cleavage sites in recovered ⌬ SH/G ...
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A vitellogenin polyserine cleavage site: highly disordered conformation protected from proteolysis by phosphorylation
... AmVg cleavage site, and used limited proteolysis to see whether (a) the natural AmVg cleavage pattern can be triggered with trypsin/chymotrypsin and (b) the digestion pattern matches the expectations ...
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