tryptophan synthetase
MUTANTS OF ESCHERICHIA COLI DEFECTIVE IN THE B PROTEIN OF TRYPTOPHAN SYNTHETASE. IV: RECOMBINATIONAL MAP
... When we found that trpA2 trpC- and trpA2 trpD- mutants could not grow on indole (unpublished results, this labora- tory), a trpA2 trpC9 double mutant was constructed i n ord[r] ...
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MUTANTS OF ESCHERICHIA COLI DEFECTIVE IN THE B PROTEIN OF TRYPTOPHAN SYNTHETASE. III. INTRAGENIC CLUSTERING
... 1970). Most of the mutants in this study were selected because of their inability to form tryptophan from indole and serine. We were initially surprised that a large proportion [r] ...
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NONALLELIC SUPPRESSORS OF A NEUROSPORA TRYPTOPHAN SYNTHETASE MUTANT
... On the basis of the similar enzymatic properties of both types of suppressed tdZo1 mutants, the site-specificity of the suppressor genes and the lack of gross effec[r] ...
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GENE INTERACTION IN TRYPTOPHAN SYNTHETASE FORMATION
... The specificity of the four suppressor genes was examined by crossing wild type strains carrying each of the suppressors to the four suppressable strains and to tdl, which w[r] ...
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EFFECT OF GENE DOSAGE ON TRYPTOPHAN SYNTHETASE ACTIVITY IN SACCHAROMYCES CEREVISIAE
... The determination of DNA content per cell performed with the aim of imple- menting the information on the macromolecular components of a polyploid series confirms OGUR’S data [r] ...
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A BIOCHEMICAL AND GENETIC STUDY OF REVERSION WITH THE A-GENE A-PROTEIN SYSTEM OF ESCHERICHIA COLI TRYPTOPHAN SYNTHETASE
... Even in those cases in which complex reversion patterns were not evident, it is possible that revertants that appear to be identical arose by different amino acid replacement[r] ...
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MUTANTS OF ESCHERICHIA COLI DEFECTIVE IN THE B PROTEIN OF TRYPTOPHAN SYNTHETASE I. SELECTION AND CLASSIFICATION
... 15 strains possessing “efficient, active” B-CRM, The B protein in these strains, though unable to convert indole to tryptophan unassisted, is sufficiently active in co[r] ...
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MUTANTS OF ESCHERICHIA COLI DEFECTIVE IN THE B PROTEIN OF TRYPTOPHAN SYNTHETASE. II. INTRAGENIC POSITION
... If Bx or By lie outside the deleted area, recombinants able to grow on indole should appear at a frequency (relative to the standard his- to his+ trans- duction) related t[r] ...
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SUPPRESSOR GENE ACTION IN THE TRYPTOPHAN SYNTHETASE SYSTEM OF NEUROSPORA CRASSA. I. GENETIC STUDIES
... Subsequently all seven inde- pendently occurring td,,, sulo, isolates were crossed to selected strains containing linkage group VI1 markers: To select against loss of th[r] ...
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SUPPRESSOR GENE ACTION IN THE TRYPTOPHAN SYNTHETASE SYSTEM OF NEUROSPORA CRASSA. II. BIOCHEMICAL STUDIES
... The enzymological properties of the suppressed enzyme, i.e., InGP utilizing activities, are so similar to those of the wild-type protein that second- site reversion or[r] ...
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STUDIES ON A SERIES OF TRYPTOPHAN-INDEPENDENT STRAINS DERIVED FROM A TRYPTOPHAN-REQUIRING MUTANT OF ESCHERICHIA COLI
... Tryptophan synthetase activity in extracts of selected suppressed strains and reuertants: To determine whether tryptophan synthetase activity is correlated with growth ra[r] ...
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ACTION OF A SUPER-SUPPRESSOR IN YEAST IN RELATION TO ALLELIC MAPPING AND COMPLEMENTATION
... Tryptophan synthetase mutants of yeast: Action of a super-suppressor in relation to allelic mapping and complementation. Thesis, University of California[r] ...
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Analysis of Core Region from Egg White Lysozyme Forming Amyloid Fibrils
... and tryptophan, respectively), forms strong aggrega- tion and fibrils in a similar manner to that for the present K peptide under the same conditions as those applied for the latter (unpublished data), and this ...
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Inborn errors in the metabolism of glutathione
... Glutathione synthetase catalyses the last step in the syn- thesis of glutathione and a deficiency results in low levels of glutathione (Figure ...cysteine synthetase in the gamma-glutamyl cycle, which ...
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COMPLEMENTATION ANALYSIS OF THE TRYPTOPHAN PATHWAY IN ASPERGILLUS NIDULANS
... The apparently identical pattern in the genetic control by trypA of anthranilate synthetase (tryp-2 in Neurospora) and by trypC of anthranilate synthetase, PRA isomerase and [r] ...
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ATP dependent ligases in trypanothione biosynthesis – kinetics of catalysis and inhibition by phosphinic acid pseudopeptides
... Trypanothione is synthesized in these medically important parasites from glutathione (GSH) and sper- midine (Spd) by a monomeric C-N ligase [trypanothi- one synthetase (TryS), EC 6.3.1.9], in a two-step reaction ...
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STUDIES ON TRYPTOPHAN PYRROLASE IN DROSOPHILA MELANOGASTER
... Tryptophan pyrrolase, the enzyme which, in the presence of an excess of ky- nurenine formamidase, converts tryptophan to kynurenine, has been followed at various developmen[r] ...
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Glutathione may have implications in the design of 3-bromopyruvate treatment protocols for both fungal and algal infections as well as multiple myeloma
... γ-glutamylcysteine synthetase and glutamine synthetase respectively, thus reducing the intracellular GSH level [16, 17], whereas paracetamol is metabolized to cytotoxic N-acetyl-4-benzoquinoneimine (NAPQI) ...
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PyrH and PrnB crystal structures
... required FADH for the catalysis of the halogenation reaction 40 . The isolation of pure PrnA was achieved in 2000 41 . In the first step of the purification protocol, a protein component absolutely necessary for the ...
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Tryptophan catabolism in acute exacerbations of chronic obstructive pulmonary disease
... acid tryptophan (Trp, m/z ...Since tryptophan catabolism has been associated with both immune modulation and infection, we sought to quantify tryptophan and its major metabolite, ...identify ...
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