two-photon excitation
Stimulated emission depletion following two photon excitation
... colour two photon stimulated emission depletion has been successfully demonstrated in fluorescein in both ethylene glycol and methanol solutions using both fully time resolved fluorescence intensity and ...
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Two photon excitation for C2V molecules based on the full relativistic theory
... Keywords: Two-Photon Excitation; C 2v Group; The Full Relativistic Quantum Mechanical Theory ...Since two-photon absorption had been theoretically described by Maria Göppert-Mayer as ...
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Wavefront assessment and correction of the rat eye for two photon excitation therapies
... One-photon excitation PDT treatment of AMD has not been found to successfully improve vision qual- ity due to the revascularization in the retina which occurs ...post-treatment. Two-photon ...
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Nanodiamonds enable adaptive-optics enhanced, super-resolution, two-photon excitation microscopy
... Figure 5a shows a pair of ND clusters imaged using two-photon excitation microscopy. This image was generated by repeating 100 times and processed by correcting for in-plane sample drift, using the ...
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Exploration of the two-photon excitation spectrum of fluorescent dyes at wavelengths below the range of the Ti:Sapphire laser
... the two- photon excitation efficiency for a number of common UV ex- citable fluorescent dyes; the nuclear stains DAPI, Hoechst and SYTOX Green, chitin- and cellulose-staining dye Calcofluor White and ...
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Development and Application of Two-Photon Excitation Stimulated Emission Depletion Microscopy for Superresolution Fluorescence Imaging in Thick Tissue
... 6 excitation techniques suffer from increased background fluorescence due to out-of-focus and scattered excitation light which degrades resolution and reduces signal-to-noise, as well as produces ...
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Three-dimensional characterizations of two-photon excitation fluorescence images of elastic fibers affected by cutaneous scar duration
... Methods: A strategy for quantitative analysis of three-dimensional (3D) elastic fibers in human cutaneous scars was designed, which included 3D reconstruction, skeleton extraction, quantitative analysis, and random ...
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Widefield two-photon excitation without scanning : live cell microscopy with high time resolution and low photo-bleaching
... single-photon excitation, n = 23 at 1 Hz, n = 21 at 10 Hz and n = 98 at 100 Hz, and for two-photon excitation, n = 30 at 1 Hz, n = 35 at 10 Hz and n = 24 at 100 Hz, where n is the ...
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Rapid Method for Detection of Influenza A and B Virus Antigens by Use of a Two Photon Excitation Assay Technique and Dry Chemistry Reagents
... Turku, Turku, Finland 3 ; and Arctic Diagnostics Oy, Turku, Finland 4 Received 18 January 2007/Returned for modification 18 March 2007/Accepted 31 August 2007 New separation-free assay methods for the rapid detection of ...
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Testing Fluorescence Lifetime Standards using Two-Photon Excitation and Time-Domain Instrumentation: Rhodamine B, Coumarin 6 and Lucifer Yellow
... Lifetime 3 (τ 3 ) – – 1.75 ± 0.07 all the samples, and low enough to avoid possible aggre- gating of molecules, or other molecular effects that might affect the absorption and emission characteristics. It should be noted ...
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Combined Two-Photon Excitation and d→f Energy Transfer in a Water-Soluble Ir(III) /Eu(III) Dyad: Two Luminescence Components from One Molecule for Cellular Imaging.
... using two in- dependent emission components from a dinuclear d/f complex is ...ing two-photon excitation of the Ir unit at 780 ...of two different analytes, inde- pendently, ...
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Coherent Anti-Stokes Raman Scattering, Second Harmonic Generation and Two-Photon Excitation Fluorescence Multimodal Microscope: Realization, Metrological Characterization and Applications in Regenerative Medicine
... iv Abstract In the frame of the research in biology and in particular in regenerative medicine, it is widely requested the ability to perform measurements that have a low impact on the observed biological systems. Many ...
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TWO-PHOTON EXCITATION FLUORESCENCE MICROSCOPY
... of two-photon pho- todamage have been ...by two- or higher photon excitation of endogenous and exogenous fluorophores with a photodamage pathway similar to that of UV ...on ...
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Efficient Singlet Oxygen Photogeneration by Zinc Porphyrin-Dimers Upon One- and Two-Photon Excitation
... strong two-photon ...of two-photon absorption cross section, σ 2 , by a focus-tunable Z-scan method, which allows for nondestructive investigation of light- sensitive ...the ...
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Development of a Novel Temperature Measurement Scheme Through the Two Photon Excitation of Krypton at North Carolina State University.
... 15 transition used. For the constant wavelength tests, roughly 250 images were recorded for both seeded and unseeded settings. For scans, a scan rate of 0.0005nm/sec was used with seeded laser operation to provide ...
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Multi-photon excitation microscopy
... Multiphoton excitation microscopy (MPE) has its roots in two-photon excitation (2PE) microscopy whose story dates back more than 70 ...of two-photon quantum transitions in atoms ...
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Two-color, two-photon imaging at long excitation wavelengths using a diamond Raman laser
... by two- photon excitation at 1080 nm and ...simultaneous two-color, two-photon imaging with this laser by using the residual pump beam for excitation of a green-emitting ...
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Simultaneous two-photon imaging and two-photon optogenetics of cortical circuits in three dimensions
... the two-photon light is spatially distributed across the entire cell body; to maintain the two-photon excitation efficiency (squared-intensity) within its cover- age area, a larger ...
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Two-photon Fluorescence Light Microscopy
... Although two-photon and confocal microscopies are very similar, two-photon microscopy has a number of ...First, two-photon excitation wavelengths are typically about twice ...
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Photon statistics excitation spectroscopy of a single two level system
... fermionic two level system (TLS) is the prototype of a quantum ...that two level systems under coherent excitation, e.g. under excitation with laser light or microwaves, are vastly stud- ied ...
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