two-photon excitation fluorescence
Single Cell Assay for Molecular Diagnostics and Medicine: Monitoring Intracellular Concentrations of Macromolecules by Two-photon Fluorescence Lifetime Imaging
... A Leica TCS SP2-AOBS Spectral Confocal Scan- ner mounted on a Leica DM IRE2 inverted fluorescent microscope was used for all imaging, spectral anal- yses, and other microscopic measurements. This mi- croscope is equipped ...
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Time resolved nanosecond fluorescence lifetime imaging and picosecond infrared spectroscopy of combretastatin A 4 in solution and in cellular systems
... on fluorescence or Raman spectroscopy [4,5]. Fluorescence methods have intrinsically high sensitivity with the ability to report on molecular processes and environmental effects [6] and depend either on the ...
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Tumor-triggered drug release from calcium carbonate-encapsulated gold nanostars for near-infrared photodynamic/photothermal combination antitumor therapy
... Finally, Two-photon luminescence images of GNS were obtained using a two-photon fluorescent microscope (Olympus FV 100, Janpan) with excitation at 780 nm and emission at 601-657 ...nm. ...
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<p>Comparative Two-Photon Fluorescence Microscopy Analysis of Riboflavin Penetration in Two Different Solutions: Dextran and Hydroxypropyl Methylcellulose</p>
... multi- photon microscope (LSM 780 microscope; Zeiss, Oberkochen, Germany) which operates pulsed infrared laser light generating images through the excitation of ...
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Two-photon laser scanning fluorescence microscopy using photonic crystal fibre
... . Two types of laser source suited to two-photon excitation exist, namely continuous wave (cw) and ultra-short pulsed (USP) lasers, the latter delivering pulses typically of sub-picosecond ...
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Monitoring Photosensitizer Uptake Using Two Photon Fluorescence Lifetime Imaging Microscopy
... steady-state fluorescence intensity still suffered from artifacts arising from the heterogeneity of tissue optical properties [8], local environment, unknown chromophores with over- lapped spectra, and the ...
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Direct Vpr Vpr Interaction in Cells monitored by two Photon Fluorescence Correlation Spectroscopy and Fluorescence Lifetime Imaging
... (Figure 6D). To further char- acterize these complexes, their molecular brightness (i.e. the number of photons emitted by a particle per second for a given excitation intensity) was compared with that of eGFP ...
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Hipke, Arthur (2016): Dual-frequency-comb two-photon spectroscopy. Dissertation, LMU München: Fakultät für Physik
... Michelson-based two-photon Fourier-transform spectroscopy was first demonstrated by Bellini et ...into two beams by a beam splitter, the two beams are delayed relative to each other, and then ...
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Exploration of the two-photon excitation spectrum of fluorescent dyes at wavelengths below the range of the Ti:Sapphire laser
... one-photon excitation (Sako et ...ing two-photon excitation potentially advantageous for cell physiology ...for two-photon microscopy is the ultrashort pulsed Ti:Sapphire ...
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Multi-photon excitation microscopy
... Multiphoton excitation microscopy (MPE) has its roots in two-photon excitation (2PE) microscopy whose story dates back more than 70 ...of two-photon quantum transitions in atoms ...
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Recent advances in hydrogen peroxide imaging for biological applications
... the two-photon process, a molecule simultaneously absorbs two photons whose individual en- ergy is only half of the energy needed to excite that mol- ecule, and then releases the energy to an ...
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Single and two photon time resolved polarised fluorescence studies of probe molecule dynamics in nematic liquid crystals
... single photon polarised fluorescence measurements have been widely used to probe molecular dynamics and photophysics in condensed phases 1-3 together with the study of local structure and dynamics in ...
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Enhanced frequency up-conversion in Rb vapor
... nm photon for every six 776 nm photons ...blue fluorescence and 776 nm beam absorption, indicating that the transfer of population to levels 5D 5/2 and 6P 3/2 is not forbidden by two- and ...
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Measurements of Absolute Atomic Nitrogen Density by Two Photon Absorption Laser Induced Fluorescence Spectroscopy in Hot Air Plasma Generated by Microwave Resonant Cavity
... the fluorescence photons detected and on background ...The fluorescence flux detected remains rather weak (lower than 1 photon per laser pulse), therefore the probability of detection of a number of ...
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Development of a Novel Temperature Measurement Scheme Through the Two Photon Excitation of Krypton at North Carolina State University.
... 4 with shot-to-shot uncertainties of 2.5% in the laminar case. In turbulent flames, the accuracy of measurements is hampered by the changing composition throughout the flame. This fluctuation makes corrections to ...
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Two-color, two-photon imaging at long excitation wavelengths using a diamond Raman laser
... the fluorescence are reported and discussed in literature, and commonly attributed to saturation of the dye or photo-bleaching, but also to stimulated emission, excited state absorption, residual one-photon ...
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Nanodiamonds enable adaptive-optics enhanced, super-resolution, two-photon excitation microscopy
... nm excitation, we were able to image a single ND over multiple hours without any loss of ...the fluorescence detected over a period of 100 s of continuous excitation of a single ND using 1 mW of ...
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Fluorescence excitation analysis by two-photon confocal laser scanning microscopy: a new method to identify fluorescent nanoparticles on histological tissue sections
... of two-photon confocal laser scan- ning microscopes (CLSMs) equipped with tunable lasers to produce spectral excitation image ...these excitation image ...a two-photon CLSM ...
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Photon statistics excitation spectroscopy of a single two level system
... thermal excitation statistics on single TLSs has not been experimentally explored, neither in atomic nor in solid state ...coherent excitation conditions are rather artificial as vir- tually all radiation ...
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Single and two photon fluorescence studies of ordered molecular systems
... degrees o f alignment are now either confirmed by more than one route or by measurements free o f assumptions (i.e. estimates o f n^ and ne). These developments allowed an extension o f polarised fluorescent probe ...
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