two-photon excitation imaging
Two-color, two-photon imaging at long excitation wavelengths using a diamond Raman laser
... exactly two Stokes shifts longer than the pump center wavelength owing to spectral broadening of the Stokes field by self-phase modulation (Murtagh et ...for two-photon imaging of biological ...
13
Combined Two-Photon Excitation and d→f Energy Transfer in a Water-Soluble Ir(III) /Eu(III) Dyad: Two Luminescence Components from One Molecule for Cellular Imaging.
... ing two-photon excitation of the Ir unit at 780 ...time imaging microscopy (PLIM) allowed the lifetime of the Ir-based emission to be measured in different parts of the ...cellular ...
8
Nanodiamonds enable adaptive-optics enhanced, super-resolution, two-photon excitation microscopy
... identical imaging conditions apart from a known aberration being applied to the ...(unaberrated) imaging conditions and decreasing values of S corresponding to increasing image degradation due to ...general ...
13
Two-photon imaging of the mammalian retina with ultrafast pulsing laser
... the TPEF signal obtained with this dispersion compensation and a 32-fs laser was nearly the same as that obtained with a 75-fs laser, possibly reflecting the presence of high-order dispersion terms, especially third- ...
19
Super resolution Imaging by Two photon Structured Illumination Microscopy
... of two-photon excitation to confocal laser scanning microscope (CLSM) leads two-photon laser scanning microscopy (TPLSM) to become an important tool in ...of two photons can ...
7
Recent advances in hydrogen peroxide imaging for biological applications
... resolution imaging with lower phototoxicity and deeper tissue penetration than confocal microscopy ...the two-photon process, a molecule simultaneously absorbs two photons whose individual en- ...
10
Exploration of the two-photon excitation spectrum of fluorescent dyes at wavelengths below the range of the Ti:Sapphire laser
... one-photon excitation (Sako et ...ing two-photon excitation potentially advantageous for cell physiology ...for two-photon microscopy is the ultrashort pulsed Ti:Sapphire ...
9
Time resolved nanosecond fluorescence lifetime imaging and picosecond infrared spectroscopy of combretastatin A 4 in solution and in cellular systems
... the excitation occurs at wavelengths shorter than 400 nm, resulting in potential problems with transmission of the exciting light through both the sample and microscope ...multiphoton excitation. For ...
21
(La0 97RE0 01Yb0 02)2O2S Nanophosphors Converted from Layered Hydroxyl Sulfate and Investigation of Upconversion Photoluminescence (RE=Ho, Er)
... to photon-phonon ...a two-photon process under 980-nm laser ...cell imaging after the surfaces were grafted with hydrophilic dextran ...
8
Monitoring Photosensitizer Uptake Using Two Photon Fluorescence Lifetime Imaging Microscopy
... sources, two-photon laser excitation takes advantage of nonlinear optical effects from near-IR ultrafast lasers to confine the imaging spot size to femto-liter volumes with less scattering ...
10
A femtosecond Raman generator for long wavelength two-photon and third harmonic generation imaging
... time-averaged excitation power of 1 mW. The high signal inten- sity at low excitation powers is a key benefit of using lower repetition rate lasers for THG imaging, since the signal level scales ...
7
Widefield two-photon excitation without scanning : live cell microscopy with high time resolution and low photo-bleaching
... widefield two-photon microscope was configured as shown in Fig ...for two-photon excita- ...direct excitation towards the specimen and to transmit ...a two-mirror periscope (BB1 ...
19
Single Cell Assay for Molecular Diagnostics and Medicine: Monitoring Intracellular Concentrations of Macromolecules by Two-photon Fluorescence Lifetime Imaging
... using two-photon excited fluorescence lifetime imaging ...proteins. Two-photon excitation in Near-Infra Red biological transparency window reduced the photo-toxicity in live ...
12
Wide field 3D optical imaging using temporal focusing for holographically trapped microparticles
... wide-field imaging in order to pro- vide depth sectioning for large transverse illumination fields, removing the need for the scan in the transverse plane com- pared to standard multiphoton ...non-linear ...
5
Energy transfer between a biological labelling dye and gold nanorods
... bioscience imaging (Helmchen et al 2005). Two-photon excitation has advantage over one-photon excitation because it has higher spatial resolution, deeper penetration and less ...
7
Multi-photon excitation microscopy
... Multiphoton excitation microscopy (MPE) has its roots in two-photon excitation (2PE) microscopy whose story dates back more than 70 ...of two-photon quantum transitions in atoms ...
14
Stimulated emission depletion following two photon excitation
... and two photon ...induced two photon fluorescence, the OPA pulses were stretched using the material dispersion provided by passage through a distilled water ...
10
Photon statistics excitation spectroscopy of a single two level system
... the photon statistics on the excitation dynamics of a single two level ...the two level system and is reso- nantly excited either with coherent laser light, or excited with chaotic light, with ...
5
On demand semiconductor source of entangled photons which simultaneously has high fidelity, efficiency, and indistinguishability
... the photon counts with a nanowire superconducting single-photon ...X photon count rates reach their first maxima at π pulses under a pumping laser power of ~16 ...the photon counts usually ...
13
Neural Imaging Using Single-Photon Avalanche Diodes
... single photon emission computed tomogra- phy (SPECT) only traces one single radiation or a general radiation, not a simultaneous double ...lifetime imaging (FLIM) ...using photon absorption and stays ...
8