Type-I alveolar cell
Development of a lung slice preparation for recording ion channel activity in alveolar epithelial type I cells
... which lack the α subunit of the ENaC channel complex die shortly after birth of alveolar flooding [13]. However, although there is a general consensus concerning the cel- lular and molecular basis of the fluid ...
10
Endothelial-monocyte activating polypeptide II disrupts alveolar epithelial type II to type I cell transdifferentiation
... the cell surface, undergoes proteolytic cleavage to a mature ≈ 22-kDa form (mEMAP II) [7-9] that functions as a potent anti- angiogenic peptide ...inhibits alveolar growth with a concomitant induction of ...
11
Lats1/2 inactivation reveals Hippo function in alveolar type I cell differentiation during lung transition to air breathing
... in cell lines and embryonic stem cells identified that YAP/ TEAD bind enhancer regions of AEC1-specific genes, including Pdpn and Ager (Zhao et ...differentiated cell type whereas basal cells are ...
10
Mechanisms of lung fibrosis induced by carbon nanotubes: towards an Adverse Outcome Pathway (AOP)
... determined. Cell name: A549: human lung adenocarcinoma alveolar epithelial cell line; ATII: primary human alveolar type-II epithelial cells; BALB-3 T3: mouse embryonic fibroblast ...
23
Gold nanoparticles induce cytotoxicity in the alveolar type-II cell lines A549 and NCIH441
... rat alveolar macrophages and epithelial type-I cells [33], and in mouse dendritic cells and fibroblasts [34], gold nanoparticles are internal- ized and stored in endocytic ...(DC2.4 cell line) ...
12
Influenza H5N1 virus infection of polarized human alveolar epithelial cells and lung microvascular endothelial cells
... human type I-like alveolar epithelial cells and lung microvascular endothelial cells by influ- enza A ...in alveolar epithelial ...polarized alveolar epithelium while the human ...
12
Alveolar Type II Epithelial Cells Contribute to the Anti Influenza A Virus Response in the Lung by Integrating Pathogen and Microenvironment Derived Signals
... are type II alveolar epithelial cells (AECII), which are increasingly recognized for their immunological ...immune cell recruitment, and antigen ...interferon I/III levels and cell ...
11
Epithelial Sodium Channels in Pulmonary Epithelial Progenitor and Stem Cells
... cells, alveolar type I (ATI) and type II (ATII) cells differentiate from these progenitor cells at different stages of gestation (Figure ...
5
Regeneration of the lung: Lung stem cells and the development of lung mimicking devices
... mature alveolar cells. However, after pneumonectomy the contribution of AT-I cells to regener- ate AT-II cells is small (~10 ...other cell sources also contribute to re-alveolarization ...progenitor ...
16
Mac-1 deficiency induces respiratory failure by affecting type I alveolar epithelial cells.
... Type I alveolar epithelial cells cover more than 95% of the alveolar surface, and are vulnerable to various ...from type II alveolar cells, the involvement of which in lung ...
9
Induced pluripotent stem cell-derived lung alveolar epithelial type II cells reduce damage in bleomycin-induced lung fibrosis
... for cell differentiation from iPSC into AEC2 has been effective with high efficiency, it is important to develop further a protocol where the differentiation will be fully ...stem cell replacement therapy ...
12
Alveolar macrophages release an insulin like growth factor I type molecule
... activated alveolar macrophages using ion exchange and gel filtration chromatography revealed an IGF-I molecule as detected by an anti-IGF-I polyclonal antibody and that the specific activity of the ...
10
Metabolism of platelet activating factor in isolated perfused rat lung
... metabolism into lyso-PAF (3.3%), phosphatidylcholine (82.3%), neutral lipid (1.7%) and intact PAF (10.2%). Analysis of tissue phosphatidylcholine by mass spectrometric techniques revealed metabolism of PAF to ...
9
Transcriptomic analysis comparing mouse strains with extreme total lung capacities identifies novel candidate genes for pulmonary function
... All procedures were approved by the Bavarian Animal Research Authority, Animal Research Authority of Schleswig-Holstein, and University of Pittsburgh, PA. Frozen and paraffin embedded tissues were procured to carry out ...
16
Rosiglitazone promotes ENaC-mediated alveolar fluid clearance in acute lung injury through the PPARγ/SGK1 signaling pathway
... various cell signaling pathways and phosphoryl- ation cascades, playing important roles in ion channels, the cellular stress response, and cell ...precursor cell-expressed developmentally downregu- ...
11
GABA receptor ameliorates ventilator induced lung injury in rats by improving alveolar fluid clearance
... Mechanical ventilators are extensively used in critical care units. High-volume ventilation causes epithelial damage, oxidative stress, surfactant inactivation, and pul- monary edema [23-26]. It also increases the ...
9
Inflammatory and cytotoxic responses of an alveolar-capillary coculture model to silica nanoparticles: Comparison with conventional monocultures
... To avoid nanoparticle aggregation predilutions of the aSNP dispersion were made in pure water (Braun ad iniectabilia, Braun Melsungen AG, Melsungen). Due to nanoparticle aggregation in serum-containing medium, serum-free ...
16
Double impact of cigarette smoke and mechanical ventilation on the alveolar epithelial type II cell
... BAL cell counts were significantly ele- ...of alveolar epithelial inflamma- tion with the combined intervention compared with either intervention ...in alveolar epithelial ...the alveolar ...
12
Steele_unc_0153D_15610.pdf
... 800 nm streptavidin coated magnetic beads (Solulink) were blocked with sterile filtered Tris buffered saline (TBS) containing 0.1% casein for 20 minutes. The beads were washed 4 times with antibody wash buffer (100 mM ...
234
Contribution of Bacterial Lipopolysaccharide to Carbon Nanotube- and Vanadium Pentoxide-Induced Pulmonary Fibrosis in Rats
... chemokines are thought to play a significant role. The Th2 cytokines IL-4 and IL-13 have, perhaps, received the most attention. Interleukins are secreted mainly by lymphocytes recruited to the lung during inflammation. ...
302