ubiquitin ligase
Putative E3 Ubiquitin Ligase of Human Rotavirus Inhibits NF κB Activation by Using Molecular Mimicry To Target β TrCP
... E3 ubiquitin ligase SCF -TrCP recognizes this phosphodegron through its  -transducin repeat-containing protein (  -TrCP) subunit and induces I B degrada- tion, allowing NF- B to translocate to the ...
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HIV relies on neddylation for ubiquitin ligase mediated functions
... HIV-1 Vpr also promotes G2 cell cycle arrest though interaction with the CRL4 ubiquitin ligase complex [17,22,36-39]. G2 cell cycle arrest is not linked to HIV-1 Vpr mediated UNG2 depletion and the ...
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The ubiquitin ligase TRIM25 targets ERG for degradation in prostate cancer
... Next, we tested the role of TRIM25 as a putative ERG-specific ubiquitin ligase using a cell-based ubiquitination assay. Because of low plasmid transfection efficiency and slow growth of VCaP cells, we ...
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The expression of the ubiquitin ligase subunit Cks1 in human breast cancer
... associated with a poor prognosis in breast cancer. The decrease in the levels of this protein is the result of increased proteasome-dependent degradation, mediated and rate-limited by its specific ubiquitin ...
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Proteomic characterization of endogenous substrates of mammalian ubiquitin ligase Hrd1
... a ubiquitin ligase that downregulates proteins via proteasomal degradation, we expected that inactivation of Hrd1 should lead to accu- mulation of substrates, as demonstrated for misfolded model ERAD ...
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Dissection of the HIV Vif Interaction with Human E3 Ubiquitin Ligase
... E3 ligase with 1:1 ...E3 ubiquitin ligase: the Vif BC box interacts with EloBC ...E3 ubiquitin ligase machinery to evade the cellular innate immune ...
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Hippo Stabilises Its Adaptor Salvador by Antagonising the HECT Ubiquitin Ligase Herc4
... The present study focuses on the tumor suppressor protein Salvador (Sav), which, together with Hpo and Wts, was one of the first Hpo pathway components to be identified [5,6]. Sav is a WW domain-containing scaffold ...
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The role of SCF ubiquitin-ligase complex at the beginning of life
... The ubiquitin ligase AGO can affect the develop- ment of tracheal system of drosophila embryo by control- ling Trh protein and its substrate Breathless protein ...
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The cochaperone and ubiquitin ligase CHIP in protein quality control
... Protein quality control is essential for living cells to maintain their normal function and to cope with stress challenges. Molecular chaperones participate in both protein folding and degradation pathways of the ...
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Cullin4A and Cullin4B Are Interchangeable for HIV Vpr and Vpx Action through the CRL4 Ubiquitin Ligase Complex
... H uman immunodeficiency virus (HIV) infection remains a se- rious threat to human health. An understanding of the mo- lecular biology underlying HIV replication and pathogenesis will be crucial for stopping this ...
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Regulation of the Centromeric Histone H3 Variant Cse4 by the E3 Ubiquitin Ligase, Pshl
... An E3 ubiquitin ligase prevents ectopic localization of the centromeric histone H3 variant via the centromere targeting domain.. de FACTo nucleosome dynamics..[r] ...
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Autographa californica Nucleopolyhedrovirus AC141 (Exon0), a Potential E3 Ubiquitin Ligase, Interacts with Viral Ubiquitin and AC66 To Facilitate Nucleocapsid Egress
... ABSTRACT During the infection cycle of Autographa californica multiple nucleopo- lyhedrovirus (AcMNPV), two forms of virions are produced, budded virus (BV) and occlusion-derived virus (ODV). Nucleocapsids that form BV ...
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The Mdm2 Ubiquitin Ligase Enhances Transcriptional Activity of Human Papillomavirus E2
... This study shows for the first time a role for a ubiquitin ligase, Mdm2, in the transcriptional activity of HPV-16 E2. This observation is supported by the abilities of Mdm2 and E2 to interact in vivo and ...
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Modulating Parkin E3 Ubiquitin Ligase Activity Using Phospho-Ubiquitin Variants
... neurodegeneration 49,50 . The ability to synthesize site-specifically phosphorylated proteins, such as pUb or recombinant active kinases 13 , enables drug-screening efforts to explore new targets and to specifically ...
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Spartin activates atrophin-1-interacting protein 4 (AIP4) E3 ubiquitin ligase and promotes ubiquitination of adipophilin on lipid droplets
... The following antibodies were used: rabbit polyclonal anti-hemagglutinin (HA; Abcam, Cambridge, MA, USA), mouse monoclonal anti-HA (12CA5; Covance, Prince- ton, NJ, USA), rabbit polyclonal anti-Myc, mouse mono- clonal ...
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The ubiquitin ligase Siah is a novel regulator of Zeb1 in breast cancer
... Siah proteins are ubiquitin ligases that regulate signal transduction by targeting substrates for proteasomal degradation [16]. To examine whether Siah proteins could alter Zeb1 protein levels, Zeb1 was ...
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Biallelic mutations in the ubiquitin ligase RFWD3 cause Fanconi anemia
... Defective HR in ΔRFWD3 DT40 cells was further examined by a modified I-SceI–induced HR assay. The recombination sub- strate SCneo (2 tandem nonfunctional neo cassettes that can be repaired by HR) was integrated at the ...
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The Poxviral RING Protein p28 Is a Ubiquitin Ligase That Targets Ubiquitin to Viral Replication Factories
... as ubiquitin ligases (RING-E3s) which simulta- neously interact with a specific substrate and a ubiquitin-con- jugating enzyme (ubc), or E2, to mediate substrate-specific ubiquitination ...of ...
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Mutually dependent degradation of Ama1p and Cdc20p terminates APC/C ubiquitin ligase activity at the completion of meiotic development in yeast
... Even less is known about how the APC/C is inactivated as cells exit meiosis II. This is an important question as APC/C inactivation is important for normal embryonic development in Drosophila [40]. Similarly, we find ...
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Degradation of Centromeric Histone H3 Variant Cse4 Requires the Fpr3 Peptidyl-prolyl Cis–Trans Isomerase
... Hewawasam, G., M. Shivaraju, M. Mattingly, S. Venkatesh, S. Martin-Brown et al., 2010 Psh1 is an E3 ubiquitin ligase that Figure 4 Fpr3 regulates the Cse4– Psh1 interaction. (A and B) Interac- tion between ...
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