Uhplc ms
Simple, sensitive and rapid determination of linifanib (ABT-869), a novel tyrosine kinase inhibitor in rat plasma by UHPLC-MS/MS
... Triple-quadruple tandem mass spectrometer equipped with electrospray ionization (ESI) interface was used for analytical detection. The detection was obtained in ESI positive mode using multiple reaction monitoring (MRM), ...
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Application of ultra high performance liquid chromatography mass spectrometry (UHPLC MS) metabolomic fingerprinting to characterise GM and conventional maize varieties
... of UHPLC-MS records obtained by the analysis of the sample sets studied, an automated algorithm was employed for the data mining and aligning ...of UHPLC-MS data obtained by the analysis of ...
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Determination of immunogenic proteins in biopharmaceuticals by UHPLC–MS amino acid analysis
... validated UHPLC–MS method for fast and reliable quantification of amino acids was tested on hydrolyzed model protein sample which was represented by bovine serum albumin (BSA) ...
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Simultaneous Determination of Four Estrogens in Compost Based on Ultrasonic Solvent Extraction, Solid Phase Extraction Clean Up and Analysis by UHPLC MS/MS
... Volumes of 5 mL of methanol were added to the spiked compost samples; then, the mixtures were vortex for 1 min, ultrasonicated for 15 min and centrifuged at 4000 rpm and 4˚C for 5 min. The supernatants were then decanted ...
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Development and application of a UHPLC-MS/MS metabolomics based comprehensive systemic and tissue-specific screening method for inflammatory, oxidative and nitrosative stress
... Several different methods have been developed for the analyses of these metabolites, each focusing on specific com- pound classes and biological matrixes. The analyses of prostanoids and isoprostanes is commonly ...
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Determination of lesinurad in rat plasma by a UHPLC–MS/MS assay
... The previously studies primarily focused on descrip- tions of pharmacokinetics and pharmacodynamics of les- inurad in healthy individuals or gout patients under given different therapeutic regimes. In these researches, ...
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Development and validation of an improved HPLC-UV method for simultaneous determination of lamotrigine and oxcarbazepine and its active metabolite 10,11-dihydro-10-hydroxycarbazepine in human blood plasma and comparison with an UHPLC-MS/MS method
... Previously, several methods were developed and vali- dated for the determination of the three analytes with many limitations. One HPLC-UV method published in 2013 used solid-phase extraction for sample pretreat- ment, ...
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Antioxidants and α glucosidase inhibitors from Neptunia oleracea fractions using 1H NMR based metabolomics approach and UHPLC MS/MS analysis
... to UHPLC–MS/MS analysis to identify the de- rivatives of the phenolics ...(RT), MS/MS data and distribution of the identified ...the MS/MS data in comparison with the ...
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<p>Pharmacokinetics and Metabolism Research of Shenkang Injection in Rats Based on UHPLC-MS/MS and UHPLC-Q-Orbitrap HRMS</p>
... novel UHPLC-MS/MS and UHPLC- Q-orbitrap HRMS method is developed to explore the pharmacokinetic and metabolism pro fi ling of the seven bioactive compounds in rat plasma and urine after intra- ...
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ANALYTICAL AND BIOANALYTICAL UHPLC MS METHOD VALIDATION FOR DETERMINATION OF METFORMIN, A BIGUANIDE AND SITAGLIPTIN, A DPP 4 INHIBITOR
... Orbitrap UHPLC-MS ...on UHPLC system, consisting of a LC-pump (Accela), degasser and ...autosampler. UHPLC system was coupled to a quadrupole-orbitrap mass spectrometer by electrospray ...
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METHOD DEVELOPMENT AND VALIDATION OF BUCLIZINE HYDROCHLORIDE ASSAY AND ITS ORGANIC IMPURITIES IN API AND PHARMACEUTICAL FORMULATION BY USING ORTHOGONAL DETECTOR TECHNIQUES (UHPLC MS) WITH DESIGN OF EXPERIMENTS
... indicating UHPLC-MS method has been developed for BH API and PF and its impurities using a systematic approach, with the aim of modeling software Design Expert@ with orthogonal detectors ...The ...
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ESTIMATION OF SITAGLIPTIN IN K2EDTA HUMAN PLASMA USING UHPLC-MS/MS METHOD
... 2.4 Liquid chromatographic optimization Sitagliptin and Sitagliptin D4 was separated on Shimadzu UHPLC on Phenomenex, Gemini C18, 3µ, 100x4.6mm column using a mobile phase composed of acetonitrile and 5mM ammonium ...
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... of UHPLC-Q-TOF ...with MS/MS-based SRM ...to UHPLC-MS/MS analysis, facilitating time and cost-efficient measurement of MC and ...
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<p>Berberine Inhibits Growth of Liver Cancer Cells by Suppressing Glutamine Uptake</p>
... metabolism, UHPLC-MS /MS analysis was performed to determine the concentration of glutamine, glutamate and glycine in berberine-treated Hep3B and BEL-7404 cells (Figure 2A and ...
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An Ultra High Performance Liquid Chromatography Tandem Mass Spectrometry Method for the Quantification of Vancomycin Requiring Only 2 µL of Rabbit Serum
... selective UHPLC-MS/MS method was developed for the quanti- fication of VAN in rabbit serum requiring 2 µL of ...current UHPLC-MS/MS method is superior to the PETINIA technique as ...
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Determination of pharmaceuticals in freshwater sediments using ultrasonic-assisted extraction with SPE clean-up and HPLC-DAD or LC-ESI-MS/MS detection
... 3 The United States Environmental Agency (USEPA) published an analytical reference method (1694) based on UAE involving two different extraction methods under acidic and basic conditions for the determination of ...
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High-throughput quantification of more than 100 primary- and secondary-metabolites, and phytohormones by a single solid-phase extraction based sample preparation with analysis by UHPLC–HESI–MS/MS
... the UHPLC–MS/MS methods (see Additional file 1: Tables S16–S22) by comparing measurements from the com- pounds of interest with or without leaf ...
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Journal of Applied Pharmaceutical Science
... Due to the development of antimalarial resistance strains of Plasmodium falciparum, the search for new antimalarial drugs is immediately needed. Such drugs might work by obstructing the heme detoxification pathway in the ...
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Identification and quantitation of related substances of super potent steroid in its topical combination drug product with vitamin D3 analogue
... The predominant analytical techniques used for the quantitative analysis of impurities in the pharmaceutical industry are high-performance liquid chromatography and ultra-high performance liquid chromatography. Several ...
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Isolation, purification and partial characterization of a glycoside from the Jamaican ball moss plant (Tillandsia recurvata L )
... For MS-MS analysis, sample fragmentation was accomplished by ...IT- MS-MS in both positive and negative ionization modes, with additional fragmentation modes (MS3 and MS4, ETD) applied to ...
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