β-ACTIN
Inhibition of ER /ERK/P62 cascades induces “autophagic switch” in the estrogen receptor-positive breast cancer cells exposed to gemcitabine
... MDA-MB-231 cells were exposed to gemcitabine, the ratio of LC3-II/LC3-I was increased in a time dependent manner (Figure 1A, 1E). Due to the degradation of LC3- II in the lysosomes, the increasing of LC3-II/LC3-I ratio ...
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Age-related sex differences in the expression of important disease-linked mitochondrial proteins in mice
... protein β-actin in pre-pubertal (1-month-old), pubertal (5- month-old), and post-pubertal (10-month-old) B6 mice of different sex at physiological ...and β-actin were influenced by ...of ...
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Successful reduction of inflammatory responses and arachidonic acid–cyclooxygenase 2 pathway in human pulmonary artery endothelial cells by silencing adipocyte fatty acid-binding protein
... The total proteins extract from HPAECs and were ob- tained using a lysis buffer (Beyotime Institute of Biotech- nology, Nantong, China). Proteins were separated by SDS-polyacrylamidegel electrophoresis in 15% gels, and ...
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Tumor promotion by γ and suppression by β non-muscle actin isoforms
... between β- and γ-actins was poorly studied because of lack of specific antibodies distinguishing these two ...γ-cytoplasmic actin null mice (Actg1-/-) dead within 48h after birth [11], complicated the study ...
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Sleep quality, BDNF genotype and gene expression in individuals with chronic abdominal pain
... ACTB: β − ACTin; APP: Amyloid- β Precursor Protein; BDNF: Brain-Derived Neurotrophic Factor; cDNA: Complementary DNA; CAP: Chronic Abdominal Pain; FDR: False Discovery Rate; GI: Gastrointestinal; ...
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Identification of Potential Biomarkers in Acquired Cholesteatoma
... cytoplasmic actin 1, also referred to as β-actin (3CL). Actin is an essencial component of the cytoskeleton, performing a critical role in a wide variety of cellular processess, including cell ...
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Metastatic pathway and the microvascular and physicochemical microenvironments of human melanoma xenografts
... Abbreviations ACTB: β-actin; ANGPT2: angiopoietin-2; B2M: β-2-microglobulin; BVD: blood vessel density; CDX models: cell line-derived xenograft models; F3: coagulation factor-III; GAPDH:[r] ...
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Qualification Study of Two Genomic DNA Extraction Methods in Different Clinical Samples
... The classic phenol-chloroform is one of the oldest DNA extraction protocols. As clarified earlier, this protocol benefits from the use of sodium dodecyl sulfate (SDS) for lysis of the cell wall, and proteinase K and ...
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Systemic vasculopathy with altered vasoreactivity in a transgenic mouse model of scleroderma
... (Rpl13); β-actin (ActB); and ubiq- uitin C (Ubc) were identified by using geNorm, and copy numbers were corrected using the computed normaliza- tion factor ...
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Oxytocin inhibits lipopolysaccharide-induced inflammation in microglial cells and attenuates microglial activation in lipopolysaccharide-treated mice
... Total RNA was extracted from cells and the prefrontal cor- tex using the Trizol reagent (Gibco, Invitrogen) according to the manufacturer’ s instructions. RNA concentration was determined using a spectrophotometer ...
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mRNA degradation: an underestimated factor in steady state transcript levels of cytochrome c oxidase subunits?
... To put these differences in context, consider the influence of decay on the mRNA levels of COX4-1 and the two housekeeping genes, β-actin and EF-1α, each assayed in fish at their respective holding ...
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Interaction of microtubules with the actin cytoskeleton via cross-talk of EB1-containing +TIPs and γ-actin in epithelial cells
... the actin isoform composition of the ...in β-actin depleted cells indeed usually correlates with frequent switching between very short periods of growth and ...In β-actin depleted cells ...
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Decreased expression of TRPV1 in renal cell carcinoma: association with tumor Fuhrman grades and histopathological subtypes
... to β -actin, densitometric measurements revealed that peritumoral tis- sues expressed significantly higher TRPV1 protein levels than different RCC histopathological subtypes (p= ...
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Molecular Physiology of an Extra-renal Cl- Uptake Mechanism for Body Fluid Cl- Homeostasis
... tetrazolium. β-actin was used as an internal ...and β-actin bands were quantified using the software Im- age-Pro Plus (Media Cybernetics, Maryland, ...
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Expressions of IGFBP-5, cFLIP in cervical intraepithelial neoplasia, cervical carcinoma and their clinical significances: a molecular pathology
... of β-actin as an internal ...The β- actin primer sequences were forward, 5'-GTGGG GCGCC CCAGG CACCA-3' and reverse 5'-GTCCT TAATG TCACG CACGA TTTC-3', which amplified a band of 540 ...
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CRISPR/Cas9 Knockout Strategies to Ablate CCAT1 lncRNA Gene in Cancer Cells
... and β-actin, quantitative RT-PCR reactions con- taining Premix Ex Taq (Probe qPCR) master mix (Takara, Japan), 2 μl cDNA template and each primer at 500 nM and 100 nM probe (dual-labeled hybridization ...
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Evaluation of Expression Stability of Candidate References Genes among Green and Yellow Pea Cultivars (Pisum sativum L ) Subjected to Abiotic and Biotic Stress
... Dry pea (Pisum sativum L.) is grown as human and animal feed throughout the world. Large yield losses in pea due to biotic and abiotic stresses compel an improved understanding of mechanisms of stress tolerance and ...
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Evidence for modulation of pericryptal sheath myofibroblasts in rat descending colon by Transforming Growth Factor β and Angiotensin II.
... factor β , (TGF β ), endothelin 1, (ET- 1) and connective tissue growth factor, (CTGF) that stim- ulate the myofibroblasts to generate collagen ...TGF- β signalling occurs through its interaction ...
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Mechanoregulation and pathology of YAP/TAZ via Hippo and non-Hippo mechanisms
... and β-catenin syner- gize in cell cycle ...with β-catenin in mouse liver leads to the development of hepatoblastoma [134], and YAP is required for cell survival in β-catenin-driven colon cancers ...
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Mice deficient in α actinin 4 have severe glomerular disease
... Focal and segmental glomerulosclerosis (FSGS) describes a pattern of renal injury observed as both a primary idiopathic entity and a secondary response to other primary disease processes (1). We previously demonstrated ...
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