β-Galactosidase
Kinetic Studies on β Galactosidase Isolated from Apricots (Prunus armeniaca kaisa)
... represent β-galactosidase ...ml. β-galactosidase was assayed by following the release of p-nitrophenol from p-nitrophenyl β-D-galactopyranoside ...of β-galactosidase was ...
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Dealing with different methods for Kluyveromyces lactis β-galactosidase purification
... Relatively high amounts of enzyme (40-80 µg of purified protein) were needed in order to detect the active band after electrophoresis on a 5-15% nondenaturing acrylamide gel. In this case β -galactosidase ...
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Secretion and properties of a hybrid Kluyveromyces lactis-Aspergillus niger β-galactosidase
... lactis β-galactosidases, a prediction of the tertiary structure of these proteins and the ...niger β - galactosidase was ...coli β- galactosidase [27,34] and which form the active-site ...
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Screening of β-Galactosidase ProducingFungi from Marine Samples
... 1995). β -galactosidases are widely distributed in all organisms, but their physiological function is not always fully ...cases β -galactosidase are able to catalyze trans- ...
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Is senescence-associated β-galactosidase a marker of neuronal senescence?
... lysosomal β-galactosidase. Therefore, an increase in SA-β-gal could be due to increased enzyme activity or ...lysosomal β-galactosidase mRNA and protein and has been linked to increased ...
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Modification of β-galactosidase for use in organic mono-phase hexanol system
... oryzae β -galactosidase were evaluated and the total en- zyme activity was determined in hexanol mono-phase system using p-nitrophenyl- β -D-galactoside as a ...the β -galactosidase for ...
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A highly inducible β-galactosidase from Enterobacter sp.
... As shown in Table S-II of the Supplementary material, the enzyme was com- pletely inhibited by N-ethylmaleimide (100 %), but not affected by DTT. The enzyme was slightly affected by 2-mercaptoethanol, enhancing ...
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Perspectives and applications of immobilised β galactosidase in food industry – a review
... of β -galactosidase stability in immobilised state (Table ...in β -galactosidase immobilisation in fibres composed of alginate and gelatin, glutaraldehyde cross-links the enzyme and gelatin ...
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Macroporous Poly(Glycidyl Methacrylate co Divinylbenzene) Polymer Particles for the Immobilization of β galactosidase from Aspergillus Oryzae
... The immobilization of β-galactosidase on poly(GMA-co-DVB) copolymer was carried out by adding an amount of polymer particles (0.0500g) to 0.5mL 0.1M citric acid buffer (pH 4.0) containing enzyme (4mg/mL). ...
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Addition of β-galactosidase boosts the xyloglucan degradation capability of endoglucanase Cel9D from Clostridium thermocellum
... The removal of the galactose moieties from the side chains of the XG substrate is a prerequisite for its com- plete hydrolysis to monosaccharides [31, 32], which makes the beneficial effects of synergism between the ...
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ENHANCEMENT OF β-GALACTOSIDASE ACTIVITY OF LACTIC ACID BACTERIA IN FERMENTED CAMEL MILK
... of β-galactosidase released out the cells by the rupture processing of all strain cultures was increased significantly ...suitable β-galactosidase activity which marked by higher ...
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Expression Vectors for Methanococcus maripaludis: Overexpression of Acetohydroxyacid Synthase and β-Galactosidase
... A series of integrative and shuttle expression vectors was developed for use in Methanococcus maripaludis. The integrative expression vectors contained the Methanococcus voltae histone promoter and multiple cloning sites ...
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MUTATIONS AFFECTING SYNTHESIS OF β-GALACTOSIDASE ACTIVITY IN THE YEAST KLUYVEROMYCES LACTIS
... (Table 2 and footnote in Table 6). The mutant may be leaky since some induction occurs at 36". Neither lactose nor galactose induced p-glactosidase activity more than two-fold in [r] ...
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A fast and sensitive alternative for β galactosidase detection in mouse embryos
... Using X-gal/FeCN assays, canonical Wnt activity has been reported in the extra-embryonic visceral endoderm of transgenic E5.5 embryos carrying the TCF/Lef-lacZ transgene (Mohamed et al., 2004). This transgene, like the ...
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LAC4 IS THE STRUCTURAL GENE FOR β-GALACTOSIDASE IN KLUYVEROMYCES LACTIS
... T o determine whether lac4 mutants produce a ,&galactosidase inhibitor, ex- tracts from three of the mutant strains were separately mixed with a n extract from t[r] ...
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Mucosal vaccine delivery by non-recombinant spores of Bacillus subtilis
... been suggested as responsible of the adhesion [30]. How- ever, in the case of β-galactosidase adsorption the role of electrostatic force has been shown to be not predominant [31]. It is possible that ...
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Analysis of Axin2 expression and function in murine models for pancreatic cancer
... Since only few tubular complexes could be observed during chronic pancreatitis, we also evaluated the for- mation of precancerous lesions after acute pancreatitis. Acute pancreatitis was induced on day 22–23 in normo- ...
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The Oct1 homolog Nubbin is a repressor of NF-κB-dependent immune gene expression that increases the tolerance to gut microbiota
... Surprisingly, we observed prominent β -galactosidase ( β -gal) reporter stain- ing in the fat body of uninfected nub 1 ; CecA1-lacZ flies (Figure 1B) compared to the control flies (Figur[r] ...
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Probiotic properties of the riboflavin producing Lactobacillus fermentum strain isolated from yoghurt sample
... Probiotics impart health benefits at a lower cost and are utilized in the developing countries like India. The strain Lb. fermentum MTCC 8711 produces riboflavin, which is an essential vitamin. In this study, we have ...
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Vol 68, No 1 (2016)
... and β-galactosidase are released as a consequence of change in outer cell-wall layers and inner membrane permeability, ...cytoplasmic β-galactosidase for up to 200 min was observed ...
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