β-galactosidase (lacZ)
Perspectives and applications of immobilised β galactosidase in food industry – a review
... of β -galactosidase stability in immobilised state (Table ...in β -galactosidase immobilisation in fibres composed of alginate and gelatin, glutaraldehyde cross-links the enzyme and gelatin ...
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A highly inducible β-galactosidase from Enterobacter sp.
... As shown in Table S-II of the Supplementary material, the enzyme was com- pletely inhibited by N-ethylmaleimide (100 %), but not affected by DTT. The enzyme was slightly affected by 2-mercaptoethanol, enhancing ...
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Screening of β-Galactosidase ProducingFungi from Marine Samples
... 1995). β -galactosidases are widely distributed in all organisms, but their physiological function is not always fully ...cases β -galactosidase are able to catalyze trans- ...
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Dealing with different methods for Kluyveromyces lactis β-galactosidase purification
... or β-galactosidase (β-D-galactoside galactohydrolase, EC ...lactis β-galactosidase arose, for example, to use it as antigen to induce specific ...lactis β-galactosidase ...
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Is senescence-associated β-galactosidase a marker of neuronal senescence?
... One of the features of cellular senescence is the activity of senescence- associated- β-galactosidase (SA-β-gal). The main purpose of this study was to evaluate this marker of senescence in aging ...
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Expression Vectors for Methanococcus maripaludis: Overexpression of Acetohydroxyacid Synthase and β-Galactosidase
... coli lacZ gene was engineered by PCR for ligation was ligated into a SacI-digested pUC18, yielding ...The lacZ product was cloned into pGEM-T, yielding puter Group, Madison, ...the lacZ fragment was ...
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A fast and sensitive alternative for β galactosidase detection in mouse embryos
... Using X-gal/FeCN assays, canonical Wnt activity has been reported in the extra-embryonic visceral endoderm of transgenic E5.5 embryos carrying the TCF/Lef-lacZ transgene (Mohamed et al., 2004). This transgene, ...
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LAC4 IS THE STRUCTURAL GENE FOR β-GALACTOSIDASE IN KLUYVEROMYCES LACTIS
... T o determine whether lac4 mutants produce a ,&galactosidase inhibitor, ex- tracts from three of the mutant strains were separately mixed with a n extract from t[r] ...
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The Role of FoxJ1 in the Development and Regulation of the Adult Neural Stem Cell Niche
... Figure 33: β -galactosidase expression is found in ependymal cells of Tamoxifen-induced adult FoxJ1 Cre::ER /Rosa26 LacZ and Nestin Cre::ER /Rosa LacZ mice. (A) High magnificatio[r] ...
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Microbial polyhydroxyalkanote synthesis repression protein PhaR as an affinity tag for recombinant protein purification
... In our previous study, PhaP, the major binding protein on in vivo PHA granules, was developed as an affinity tag used for recombinant protein purification [23]. In this paper, PhaR replaced PhaP for the purification ...
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Sebaceous lipids are essential for water repulsion, protection against UVB induced apoptosis and ocular integrity in mice
... for β -galactosidase activity revealed that Cre activity is restricted to the sebaceous gland in both newborn (P4) and adult (P60) mice in both back and tail skin, with no staining in the hair follicle, ...
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Effect of Ethinylestradiol on hsp70 Expression in Transgenic Drosophila melanogaster (hsp70-lacZ) Bg9
... of β-galactosidase at this concentration was significant ...of β-galactosidase for 6, 24 and 48 hrs of exposure compared to the control (Table ...
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Analysis of Axin2 expression and function in murine models for pancreatic cancer
... b β‑galactosidase staining in tubular com‑ plexes of the pancreas in normoglycemic and hyperglycemic Axin2 +/ lacZ mice was observed after pancreatitis, whereas no β‑galactosidase ...
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Changes in Activities of Key Enzymes in Sugarcane Stem at Different Growing Stages
... and β-galactosidase may be related to cell wall loosening ...α-mannosidase, β-glucosidase and β-mannosidase increased with the elongation of sugarcane internodes, but the degrees and trends of ...
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Soil hydrolase activities and kinetic properties as affected by wheat cropping systems of Northeastern China
... α-galactosidase, β-glucosidase, α-glucosidase, urease, protease, phosphomonoesterase, phosphodiesterase, arylsulphatase) and five enzymes kinetic characters were ...(α-galactosidase, ...
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Biochemical characterisation of α and β glucosidases and α and β galactosidases from red palm weevil, Rhynchophorus ferrugineus (Olivier) (Col : Curculionide)
... and β-glucosidases and α- and β-galactosidases in the digestive system and haemolymph of this ...and β-galactosidase were higher than those of β-glucosidase and ...and ...
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Non contiguous-finished genome sequence and description of Peptoniphilus obesi sp. nov.
... α-fucosidase, β-galactosidase, glutamic acid de- carboxylase, 6-phospho-β-galactosidase β- glucosidase, β-glucuronidase, N-acetyl-β- glucosaminidase, D-mannose, ...
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Differentiation of neuronal cells using a murine embryonic stem cell based method
... Since activity of the necdin promoter conferred β-galactosidase expression, a quantitative measurement of β-galactosidase activity [21] was performed using cell lysate cells exposed to c[r] ...
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Non-contiguous finished genome sequence and description of Kallipyga massiliensis gen. nov., sp. nov., a new member of the family Clostridiales Incertae Sedis XI
... for β-galactosidase, β- galactosidase-6-phosphate, α-arabinosidase, β- glucuronidase, N-acetyl-β-glucosaminidase, glutamic acid decarboxylase, proline arylamidase, leucyl gly- ...
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An Org 1–Tup transcriptional cascade reveals different types of alary muscles connecting internal organs in Drosophila
... (B,C) β -gal staining shows the specific decrease of tupADME-lacZ expression in dorsal muscles (bracketed in B) when the Tup binding site is mutated ...
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