viral genomes
CODEHOP mediated PCR – A powerful technique for the identification and characterization of viral genomes
... of viral genomes containing multiple genes, tar- geting the divergent locus B of macaque rhadinoviruses ...new viral species, and has provided critical information regarding the gene structure and ...
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Human Papillomaviruses Preferentially Recruit DNA Repair Factors to Viral Genomes for Rapid Repair and Amplification
... lated using calcium-induced differentiation, which generates a uniformly differentiated population of cells. In keratinocytes with HPV episomes, amplification begins at 48 h after the calcium switch and plateaus at 72 to ...
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Sequencing viral genomes from a single isolated plaque
... Viral genomes were sequenced with the 454 FLX Titanium platform. Library construction, emulsion PCR (emPCR), enrichment and 454 sequencing were performed by following the vendor’s standard protocols, with ...
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Herpesvirus Replication Compartments Originate with Single Incoming Viral Genomes
... several viral genomes are needed to establish a functional ...many viral genomes are found in a single RC, we infected cells with mixtures of PRV151 and PRV BeBlu containing both in equal ...
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Defective Viral Genomes Alter How Sendai Virus Interacts with Cellular Trafficking Machinery, Leading to Heterogeneity in the Production of Viral Particles among Infected Cells
... FL genomes interacted similarly with this intracellular trafficking machinery, we performed immunofluorescence analysis for SeV NP as a proxy for vRNPs in conjunction with antibodies (Abs) targeting a variety of ...
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Unexpected invasion of miniature inverted-repeat transposable elements in viral genomes
... host genomes due to elimination or inacti- vation [9, ...in viral genomes, apart from one Tc1/mariner member recently discovered in the genome of Pandoravirus salinus [6], display very low copy ...
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A Direct Transposon Insertion Tool for Modification and Functional Analysis of Viral Genomes
... Conclusions. We described the use of several mini-Mu transposons for the facile discrimination of essential and non- essential regions and genes in the PRD1 genome. In addition, our analysis yielded critical new ...
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Identification of emerging viral genomes in transcriptomic datasets of alfalfa (Medicago sativa L )
... ticularly viral sequences [11, ...complete viral genomes from tran- scriptomic data improves our knowledge of the diversity and host range of these pathogens and provides essential tools for their ...
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Standards for Sequencing Viral Genomes in the Era of High Throughput Sequencing
... Countermeasure development. Advancements in our capa- bilities to sequence viral genomes are changing the way we coun- teract global pandemics and acts of bioterrorism. There are two important aspects of ...
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Chemical Methylation of RNA and DNA Viral Genomes as a Probe of In Situ Structure
... To characterize the sites of DMS alkylation in doublestranded and single-stranded RNAs, genome RNA that was phenol extracted from purified reovirus and viral mRNA synthesized in vitro by[r] ...
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Colobus type C virus: molecular cloning of unintegrated viral DNA and characterization of the endogenous viral genomes of Colobus.
... The EcoRI fragments region and the 3.8-kb band hybridized better to of CPC-1, including a 3.1-kb fragment from the the D probe, indicating that its homology ex- major circular form, were[r] ...
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5' Terminus of defective and nondefective Sendai viral genomes is ppp Ap.
... The products of the SV digest of the 14S terminus were separated by high-voltage paper electrophoresis, and two products were gener427 Downloaded from http://jvi.asm.org/ on November 10,[r] ...
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A novel method for efficient amplification of whole hepatitis B virus genomes permits rapid functional analysis and reveals deletion mutants in immunosuppressed patients.
... DNA polymerase (2). Unfortunately, several of these poly- merases (Vent DNA polymerase [New England Biolabs], UlTma DNA polymerase [Perkin-Elmer], and Pfu DNA poly- merase [Stratagene]) were found to be at least ...
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Identification of Small Endogenous Viral Elements within Host Genomes
... +ssRNA viral genome is tran- scribed into linear dsDNA by the viral reverse transcriptase (RT) ...the viral integrase encoded by the pol ...the viral DNA into the nuclear host DNA to form a ...
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Genetic Analysis of Serum-Derived Defective Hepatitis C Virus Genomes Revealed Novel Viral cis Elements for Virus Replication and Assembly
... virus genomes into 7 major genotypes (GTs) and many more subgenotypes (1, ...defective viral genomes (DVGs) during HCV replication further increases HCV genetic ...infectious viral particles ...
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State of the viral DNA in rat cells transformed by polyoma virus. I. Virus rescue and the presence of nonintergrated viral DNA molecules.
... These transformed cells do not produce infectious virus spontaneously but contain, in addition to integrated viral genomes, "free" viral DNA molecules.. MATERIALS AND METHODS Cell lines.[r] ...
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Cloning and characterization of a papillomavirus associated with papillomas and carcinomas in the European harvest mouse (Micromys minutus).
... The detection of viral genomes in normal tissues of harvest mice bears a striking resemblence to MnPV infection, in which viral DNAs are detected in embryos and normal tissues and increa[r] ...
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Green Fluorescent Protein-Tagged Adeno-Associated Virus Particles Allow the Study of Cytosolic and Nuclear Trafficking
... of viral trafficking, we genetically incorporated enhanced green fluorescent protein (GFP) into the adeno-associated virus (AAV) capsid by replacement of wild-type VP2 by GFP-VP2 fusion ...displaying viral ...
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Metagenomic Analysis of the Viromes of Three North American Bat Species: Viral Diversity among Different Bat Species That Share a Common Habitat
... complex viral ecology that plays a role in the emergence of infectious disease and in spread of virulence ...full-length viral genomes were ...entire viral genomic sequences that can be used ...
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Search | Preprints
... the number of viral genomes (of the same virus) is in an infected cell, the more stringent is the 296. bottleneck[r] ...
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