Viral RNA synthesis analysis
Mutational Analysis Reveals a Noncontractile but Interactive Role of Actin and Profilin in Viral RNA-Dependent RNA Synthesis
... negative-strand RNA genome of respiratory syncytial virus (RSV), a significant pediatric pathogen, absolutely requires actin and is stimulated by the actin-regulatory protein ...of viral RNA ...the ...
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Constraints of Viral RNA Synthesis on Codon Usage of Negative-Strand RNA Virus
... Negative-strand RNA viruses (NSVs) include some of the most patho- genic human viruses ...NSV RNA synthesis in that the genomic RNA sequestered in the nucleocapsid serves as the ...genomic ...
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Dynamics of Viral RNA Synthesis during Measles Virus Infection
... each viral gene during the course of an ...simultaneous analysis of numerous ...total RNA was isolated at 48 hpi. The RNA was reverse transcribed with random hexamers and oligo(dT), L-Tr-posi- ...
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Two murine coronavirus genes suffice for viral RNA synthesis.
... Sequence analysis of DIssA and its subgenomic RNA dem- onstrated that most of the DIssA molecules contained a struc- ture in which the 5 9 -end two-thirds of mRNA 2-1 was fused with an mRNA 7 that lacked ...
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Initiation of Genomic Plus-Strand RNA Synthesis from DNA and RNA Templates by a Viral RNA-Dependent RNA Polymerase
... quantitative analysis of the interaction between DNA templates and the BMV RdRp was ...reduce synthesis by 50% (IC 50 ...affect RNA synthesis at a level beyond stable inter- action with the ...
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Norovirus RNA Synthesis Is Modulated by an Interaction between the Viral RNA-Dependent RNA Polymerase and the Major Capsid Protein, VP1
... antigenomic RNA synthesis from the genomic plus-strand for use as templates for the synthesis of both genomic and subgenomic ...NoV RNA synthesis relative to other processes needed for ...
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Influenza A virus RNA-dependent RNA polymerase: analysis of RNA synthesis in vitro.
... virus RNA-dependent RNA polymerase, purified from virion ribonucleoprotein particles and from which endogenous genomic RNA (vRNA) has been depleted by treatment with micrococcal nuclease, was used to ...
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Mutational Analysis of Bovine Viral Diarrhea Virus RNA-Dependent RNA Polymerase
... FIG. 7. Schematic diagram depicting all alanine substitutions and deletion mutations in BVDV NS5B. Underlined letters represent residues that were changed to alanine by site-directed mutagenesis (vertical arrows); ...
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An RNA Pseudoknot in the 3′ End of the Arterivirus Genome Has a Critical Role in Regulating Viral RNA Synthesis
... plus-strand RNA viruses, the genome initially serves as the template for both translation of the viral replicase gene and synthesis of minus-strand RNA and is ultimately packaged into progeny ...
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Membrane Requirements for Uridylylation of the Poliovirus VPg Protein and Viral RNA Synthesis In Vitro
... PV RNA is associated with these vesicular membranes, which contain all viral and cellular components required for replication of the viral genome (11–13), and EM autoradiography demonstrated direct ...
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Ambisense Sendai Viruses Are Inherently Unstable but Are Useful To Study Viral RNA Synthesis
... eggs at first and then regained wild-type growth (by P4 in this case, due to the much more efficient BSR-T7 recovery system [see Materials and Methods]). The titers of these stocks were determined by counting infectious ...
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Staufen1 Interacts with Multiple Components of the Ebola Virus Ribonucleoprotein and Enhances Viral RNA Synthesis
... Immunofluorescence analysis (IFA). HuH-7 cells grown on 8-well chamber slides were fixed with 4% paraformaldehyde (PFA) for 15 mins, permeabilized with 0.5% Triton X-100 for 10 min, and incubated in blocking ...
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One retroviral RNA is sufficient for synthesis of viral DNA.
... We used previously characterized spleen necrosis virus-based retroviral vectors and helper cells to study the strand transfers that occur during the reverse-transcription phase of a sing[r] ...
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Gliotoxin: inhibitor of poliovirus RNA synthesis that blocks the viral RNA polymerase 3Dpol.
... This result contrasts with the mode of action of other inhibitors of poliovirus RNA synthesis, such as guanidine or flavones, that selectively block plus-stranded RNA synthesis and sugge[r] ...
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Terminal Nucleotidyl Transferase Activity of RecombinantFlaviviridae RNA-Dependent RNA Polymerases: Implication for Viral RNA Synthesis
... that RNA LE19 and LE19⫹ 1G accepted different terminal nucleotides suggests that nu- cleotide selection and incorporation are affected by the RNA ...to RNA LE19, the majority of the RNAs in this ...
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Isolation of poliovirus 2C mutants defective in viral RNA synthesis.
... linker insertion: 2C-31 and 2C-32. Figure 1 shows the loca- tions of linker insertions into the 2C region of poliovirus cDNA constructs and the corresponding changes in amino acid sequen[r] ...
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Viral RNA Synthesis and Levels of DNA-Dependent RNA Polymerases During Replication of Adenovirus 2
... (D) Endogenous levels of RNA polymerase III activity in nuclei from infected (0) and mock-infected (A) cells assayed at 0.02 M ammonium sulfate.. The individual RNA polymerase I, II, and[r] ...
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Analysis of astrovirus serotype 1 RNA, identification of the viral RNA-dependent RNA polymerase motif, and expression of a viral structural protein.
... When this motif was aligned with other known viral RNA polymerases, the astrovirus serotype 1 deduced amino acid sequence showed.. greatest similarity with the polymerase motifs of virus[r] ...
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Poliovirus mutant that contains a cold-sensitive defect in viral RNA synthesis.
... Patterns of protein synthesis in mock-, mutant 3A-2-, and wild-type-infected Vero cells at high or low temperature. Cells were pulse labeled with [35S]methionine at various times, and ce[r] ...
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Complementary Mutations in the N and L Proteins for Restoration of Viral RNA Synthesis
... sequestered RNA, without disrupting the nucleo- capsid ...of viral RNA synthesis in a minigenome ...the viral genome, mutant viruses were recovered by reverse genetics and serial ...
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