Viruses and host shutoff
Expression and function of the equine herpesvirus 1 virion-associated host shutoff homolog.
... of host protein synthesis and regulating viral mRNA turn- ...since host shutoff activity appears minimal in cytolytic EHV-1 infection, it is possible that the ORF19 gene product (and, by analogy, the ...
9
Host Shutoff Is a Conserved Phenotype of Gammaherpesvirus Infection and Is Orchestrated Exclusively from the Cytoplasm
... This host shutoff phenotype is driven by the conserved herpesviral alkaline exonuclease, termed SOX in KSHV and BGLF5 in EBV, which in gammaherpesviruses has evolved the genetically separable ability to ...
13
Herpes Simplex Virus Virion Host Shutoff Attenuates Establishment of the Antiviral State
... properties, viruses with vhs deleted have been proposed as live-attenuated ...vhs-deficient viruses replicate to reduced levels in interferon (IFN)-primed cells and that this deficit has both IFN-dependent ...
9
Role of the VP16-Binding Domain of vhs in Viral Growth, Host Shutoff Activity, and Pathogenesis
... idea. Viruses deleted in the VP16 protein show increased levels of translational arrest, and vhs activity is dampened in cells constitutively expressing VP16, suggesting that VP16 downregulates the activity of the ...
11
Pathogenesis of Herpes Simplex Virus Type 2 Virion Host Shutoff (vhs) Mutants
... Measurement of vhs activity. The vhs mutants 333 -vhsB and 333d41 were examined for their capacity to degrade GAPDH in parallel with wild-type HSV-2 (SB5) and 333-vhsB R . Mono- layer cultures of Vero cells were mock ...
8
Role of the virion host shutoff (vhs) of herpes simplex virus type 1 in latency and pathogenesis.
... 2 viruses therefore represented authentic, although decreased, amounts of replicated ...2 viruses are impaired in their levels of replication in these tissues, the deficit seen in the peripheral nervous ...
8
Characterising the role of murine gammaherpesvirus-68 host shutoff protein in mediating cellular mRNA degradation
... 94 3.3.4 Growth characteristics of ORF37Stop and revertant viruses in IFNαβRKO mice Once it had been established that the mutant virus did not show any attenuated growth in vitro (Figure 3.3), the next step was to ...
201
Venezuelan Equine Encephalitis Virus Disrupts STAT1 Signaling by Distinct Mechanisms Independent of Host Shutoff
... the shutoff of host gene expression (3, 5, 21, 23) and that this activity may involve the disruption of nuclear import of host factors (8, ...with viruses encoding the full-length VEEV genome ...
11
Herpes Simplex Virus Virion Host Shutoff Protein: Immune Evasion Mediated by a Viral RNase?
... James R. Smiley* Department of Medical Microbiology & Immunology, University of Alberta, Edmonton, Alberta, Canada T6G 2S2 Herpes simplex virus type 1 (HSV-1) is the prototypical mem- ber of the Herpesviridae, a large ...
6
Herpes Simplex Virus Virion Host Shutoff (vhs) Activity Alters Periocular Disease in Mice
... consequences for viral pathogenesis in a murine model. The entire vhs open reading frame (ORF) of HSV-1 strain KOS was exchanged with that of HSV-2 strain 333 to create the recombinant KOSV2. KOSV2 was then tested for ...
7
Going against the Tide: Selective Cellular Protein Synthesis during Virally Induced Host Shutoff
... many viruses cause host shutoff, most past efforts to understand the phenomenon were devoted to determining how a viral infection shuts off cellular protein synthesis while at the same time shunting ...
5
Herpes simplex virus virion host shutoff function.
... Vero cells were mock infected (lanes 1 and 18) or infected with passage 4 virus derived from transfections receiving wt KOS virus DNA alone (lanes 2 and 3), vhsl mutant virus DNA alone ([r] ...
10
The herpes simplex virus virion host shutoff function.
... The virion host shutoff (vhs) function of herpes simplex virus (HSV) limits the expression of genes in the infected cells by destabilizing both host and viral mRNAs.. vhs function mutant[r] ...
6
Roles of the Early Genes of Bacteriophage T7 in Shutoff of Host Macromolecular Synthesis
... A trivial explanation for the apparent dependence of late enzyme synthesis on gene 1.1 activity could be that a significant level of transcription of the late genes is carried out by act[r] ...
11
Nucleotide host markers in the influenza A viruses
... influenza viruses to cross species, various amino acid host markers in influenza viruses were ...acid host markers in ten proteins of 2009 pandemic ...cleotide host makers in the ten ...
16
Host factors against plant viruses
... on host resources and ...pro-viral host factors during the infection cycle and are targeted by antiviral ...process, host factors with antiviral activity have been ...of host fac- tors with ...
15
Charting the host adaptation of influenza viruses.
... mammalian viruses are spread out, suggesting an ongoing adaptation ...pre-1918 host-shift event was a typical or exceptional avian virus, we calculated the fraction of avian viruses that were less ...
14
Evidence for Translational Regulation by the Herpes Simplex Virus Virion Host Shutoff Protein
... vhs-induced host shutoff during HSV infection of a variety of cell ...of host mRNAs are strongly depleted during infection (54, ...on host mRNA degradation; however, it is also possible that ...
9
Control of mRNA stability by the virion host shutoff function of herpes simplex virus.
... If the Mhs gene product also regulates the half-lives of alpha mRNAs during lytic infections in the absence of drugs, one would expect the vhsl mutation to affect the accumulation of alp[r] ...
10
MUTATIONS OF BACTERIAL VIRUSES AFFECTING THEIR HOST RANGE
... that succeed in giving plaques on the resistant bacteria should be proportional to the number of virus particles tested. This will be true whether these par- ticles come from th[r] ...
16