VNTR analysis
Genotyping of Salmonella Typhi using 8-loci multi locus VNTR analysis
... repeats analysis (MLVA) are often used for the molecu- lar typing of ...of VNTR loci and the accurate siz- ing of PCR products are important ...of VNTR analysis using microfluidics chip-based ...
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Multiple locus VNTR analysis highlights that geographical clustering and distribution of Dichelobacter nodosus, the causal agent of footrot in sheep, correlates with inter country movements
... To date, molecular analyses of D. nodosus have focused on anal- ysis of isolates from a single country and included pulsed-field gel electrophoresis (PFGE), infrequent restriction site PCR (IRS-PCR) and restriction ...
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Identification of Variable Number Tandem Repeat (VNTR) Sequences in Acinetobacter baumannii and Interlaboratory Validation of an Optimized Multiple Locus VNTR Analysis Typing Scheme
... Guidelines for appropriate use and evaluation of microbial epidemiologic typing methods were followed throughout the study (33). The reproducibility (R) of the method was assessed at both the inter- and intralaboratory ...
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Identification of Variable Number Tandem Repeat (VNTR) Sequences in Legionella pneumophila and Development of an Optimized Multiple Locus VNTR Analysis Typing Scheme
... more markers, namely Lpms_1b, Lpms3, and Lpms17, for which the half-sized alleles could easily be distinguished from the full-length counterparts, improves the informativeness of the assay (HGDI ⫽ 0.93, 36 genotypes) ...
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Francisella tularensis Strain Typing Using Multiple Locus, Variable Number Tandem Repeat Analysis
... multilocus VNTR analysis (MLVA) typing system for this ...six VNTR loci in a set of 56 isolates from California, Oklahoma, Arizona, and Oregon and the ...Cluster analysis identified two ...
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High Genetic Diversity among Mycobacterium avium subsp paratuberculosis Strains from German Cattle Herds Shown by Combination of IS900 Restriction Fragment Length Polymorphism Analysis and Mycobacterial Interspersed Repetitive Unit Variable Number Tandem Repeat Typing
... MIRU-VNTR analysis, as they are two fully independent genotyping ...MIRU-VNTR analysis, IS900 RFLP as well as PFGE provides information about alterations in the whole genomes of ...
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Variable Number Tandem Repeats That Are Useful in Genotyping Isolates of Salmonella enterica subsp enterica Serovars Typhimurium and Newport
... applying VNTR analysis to the isolates that share the frequently encountered serotypes Ty- phimurium and ...six VNTR loci in less than 1 day for the cost of reagents for PCR and agarose gel ...
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Multilocus Variable Number Tandem Repeat Typing of Mycobacterium ulcerans
... locus VNTR analysis shows the Asian genotype (Japan-China) to be much more closely related to the Mexican genotype than to the Surinam ...between VNTR-based and MLST- based (21) groupings indicates ...
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Comparison of Multiple Locus Variable Number Tandem Repeat Analysis with Pulsed Field Gel Electrophoresis Typing of Acinetobacter baumannii in China
... macrorestriction analysis with pulsed-field gel electrophoresis (PFGE) has been used extensively, and it can be considered, in some instances, the gold standard for ...(VNTR) analysis (MLVA), based ...
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Automated High Throughput Genotyping for Study of Global Epidemiology of Mycobacterium tuberculosis Based on Mycobacterial Interspersed Repetitive Units
... Automated MIRU-VNTR analysis. For each multiplex PCR, 1 l of 20-fold- diluted PCR products was added to a loading buffer containing 1.25 l of deionized formamide (Amresco, Solon, Ohio), 0.5 l of blue ...
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Comparison of a Variable Number Tandem Repeat (VNTR) Method for Typing Mycobacterium avium with Mycobacterial Interspersed Repetitive Unit VNTR and IS1245 Restriction Fragment Length Polymorphism Typing
... or VNTR typing, is recommended ...IS1245-RFLP analysis cannot be used for these ...that VNTR analysis is applicable to these ...and VNTR loci, Kazumi et ...using VNTR (10). ...
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Clostridium difficile Genotypes in Piglet Populations in Germany
... multiple-locus VNTR analysis ...neighborhood analysis, BioNumerics software was used (v. 6.6). Cluster analysis was calculated based on the categorical coefficient and unweighted pair group ...
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Characterization of Ancestral Mycobacterium tuberculosis by Multiple Genetic Markers and Proposal of Genotyping Strategy
... MIRU-VNTR analysis showed that the ancestral isolates were the most heterogeneous genogroup among the genotypes iden- tified in Singapore ...MIRU- VNTR typing ...
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Development of a Multiple-Locus Variable number of tandem repeat Analysis (MLVA) for Leptospira interrogans and its application to Leptospira interrogans serovar Australis isolates from Far North Queensland, Australia
... for VNTR loci from these ...used. VNTR is therefore applicable to a wide range of laboratories, including those which may have simple equipment such as thermal cyclers and agarose gel electrophoresis but do ...
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Use of Variable Number Tandem Repeat Typing To Differentiate Mycobacterium tuberculosis Beijing Family Isolates from Hong Kong and Comparison with IS6110 Restriction Fragment Length Polymorphism Typing and Spoligotyping
... to VNTR analysis, spoligotyping, and IS6110 restriction fragment length polymor- phism (RFLP) ...14 VNTR loci included in the analysis varied from 0 to ...of VNTR analysis was ...
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Application of Multilocus Variable Number Tandem Repeat Analysis for Molecular Typing of the Agent of Leptospirosis
... (VNTR) analysis have been described and have been used to identify Leptospira interrogans ...multilocus VNTR analysis (MLVA) identification of strains to species other than ...for VNTR ...
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Genotypes and drug susceptibility of Mycobacterium tuberculosis Isolates in Shihezi, Xinjiang Province, China
... Methods: From June 2010 to June 2011, 55 M. tuberculosis isolates from patients with pulmonary TB were studied by Beijing family-specific PCR (detection of the deletion of region of difference 105 [RD105]), and ...
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Phylogeographic reconstruction of a bacterial species with high levels of lateral gene transfer
... (VNTR) analysis (MLVA) of ...hybridization analysis of 23 strains from these three Burkholderia species avoided problems associated with limited gene sampling by targeting close to 7,000 open reading ...
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Strain Typing of Borrelia burgdorferi, Borrelia afzelii, and Borrelia garinii by Using Multiple Locus Variable Number Tandem Repeat Analysis
... multiple-locus VNTR analysis (MLVA) typing system for this disease ...Cluster analysis by the unweighted pair-group method with arithmetic means resolved the 30 observed unique Borrelia genotypes ...
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Evaluating the impacts of stressors of Pseudomonas syringae pathovar tomato on the effectiveness of multi-locus variable number tandem repeat analysis and multi-locus sequence typing in microbial forensic investigations
... (VNTR) analysis (MLVA) and multi-locus sequence typing (MLST) in investigations involving plant pathogens may be problematic because the long lag periods between pathogen introduction and discovery of ...
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