Western-blot analysis
Utility of Western Blot Analysis for the Diagnosis of Cutaneous Leishmaniasis
... Serological methods have been commonly used to diagnose visceral leishmaniasis (VL) and considered as a routine method for diag- nosis of VL (11-14). Serological methods have been considered of limited importance for the ...
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Voltage profile generation for simultaneous multi protein detection in western blot analysis
... The multi-protein detection device was developed and validated. The device was capable of generating five to seven membranes from a single gel. The multi-anode plate was regulated to provide a proper voltage profile that ...
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Western Blot Analysis of Sera Reactive to Human Monocytic Ehrlichiosis and Human Granulocytic Ehrlichiosis Agents
... 15). Western immunoblot studies identified multiple bands of 23- to 30-kDa major outer membrane proteins encoded by a multigene family and a 120-kDa protein as candidate antigens for sensitive and specific ...
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Western blot analysis of antibody response to pneumococcal protein antigens in a murine model of pneumonia.
... To detect new antigen candidates for serological tests, we studied the antibody response to pneumococcal protein antigens in mice infected intratracheally with various Streptococcus pneumoniae strains. Sera were tested ...
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Western Blot Analysis of Leishmania infantum Antigens in Se-ra of Patients with Visceral Leishmaniasis
... In the present study, we used immunoblotting technique with total protein antigen extracted of L. infantum for detection of specific antibodies in patients infected with VL in the acute and chronic phase of the dis- ease ...
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Evaluation of immunoglobulin M western blot analysis in the diagnosis of congenital syphilis
... Of the 21 infants with symptomatic congenital syphilis who had further studies done, 16 had reactive FTA-ABS IgM tests compared with 19 who had reactive serum blots 18 of whom had reacti[r] ...
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A Putative Rhamnogalacturonase Required for Sexual Development of Neurospora crassa
... Western blot analysis Western blotting was per- formed to characterize the ASD-1 polyclonal antibodies (raised to a potentially antigenic peptide of the ASD-1 protein) and [r] ...
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HtrA1 Is Specifically Up-Regulated in Active Keloid
... Western blot analysis of HtrA1 in keloid lesions and normal skin tissues.. Soluble protein 129.[r] ...
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<p>The Therapeutic Effect of Melatonin on GC by Inducing Cell Apoptosis and Autophagy Induced by Endoplasmic Reticulum Stress</p>
... (A) Western blot analysis of the ER stress associated with protein ...(B) Western blot analysis of ER stress and autophagy related proteins expression following treating with the ...
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Quantitative evaluation of signaling events in Drosophila S2 cells
... Drosophila activates a robust defense response to gram-negative bacteria through the Immune deficiency (Imd) pathway. Imd signaling proceeds through c-Jun N-terminal Kinase (JNK), NF-kB and caspase modules. The ...
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Cloning, sequence analysis and expression of ovine CD154 (CD40 ligand)
... oCD154, Western Blot analysis was performed with the human CD154 specific monoclonal ...The Western Blot analysis with indicated a molecular weight of 23 kDa for the ovine CD154 ...
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Comparison of Protein Profiles in Sputum between COPD and Acute Exacerbation of COPD
... Cyclophilin B is a cyclosporine-binding protein ex- pressed mainly within the endoplasmic reticulum. Cyc- lophilin B also binds to lymphocytes [13] and may regu- late cyclosporine-mediated immunosuppression. We could not ...
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SIRT7 suppresses the epithelial-to-mesenchymal transition in oral squamous cell carcinoma metastasis by promoting SMAD4 deacetylation
... WB analysis for SIRT7 protein expression between between Group P, M, NC, n = 5 each ...WB analysis mentioned ...of western blot analysis for SIRT7 protein expression between metastatic ...
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HCMV Promotes Breast Cancer Metastasis: Impacts of CMVIL-10 in the Tumor Microenvironment
... Initial analysis of optimizations of knockdown of mDax-1 protein in siRNA treated mESC was confirmed by western blot ...by western blot analysis as a measure of cell viability ...
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The thioredoxin system of Helicobacter pylori
... SDS-PAGE Analysis of Thioredoxin Ex- pression by ...PAGE analysis/Western blot analysis of the time course of expression of intracellular thioredoxin before, and for various periods of ...
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High Level Expression of Recombinant Ribosomal Protein (L7/L12) from Brucella abortus and Its Reaction with Infected Human Sera
... During the infection by intracellular bacteria such as Brucella and Mycobacteria, some of the bacterial structural components, such as the L7/L12 ribosomal protein, were involved in the immune response developed in the ...
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Infectivity enhancement by human immunodeficiency virus type 1 Nef is independent of its association with a cellular serine/threonine kinase.
... Nef encoded by the 233 primary isolate does not bind NAK. SF2 and SIVmac239 Nef bind to and activate a cellular serine/ threonine kinase (NAK) (48). We have previously reported that the ability to coimmunoprecipitate NAK ...
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Identification and characterization of a Streptococcus equi ssp. zooepidemicus immunogenic GroEL protein involved in biofilm formation
... Confirmation of the immunogenicity of selected proteins The GroEL chaperone protein was selected for molecu- lar cloning and its immunogenicity was confirmed. The primer pairs of GroEL-S (5 ′ -CGCGAATTCGATA ...
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Original Article Synergistic role between Cul1 and PARP1: prognostic and predictive biomarkers in colorectal cancer
... Western blot and immunohistochemical staining in TMA were used to detect Cul1 and PARP1 expression in primary CRC and adjacent normal ...tissues. Western blot analysis indicated that ...
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Comparison of a Multiplexed Herpes Simplex Virus Type-Specific Immunoglobulin G Serology Assay to Immunoblot, Western Blot, and Enzyme-Linked Immunosorbent Assays
... the Western blot is still considered the “gold stan- dard” for type-specific serological diagnosis of HSV, the cum- bersome nature of the assay, along with the unavailability of a commercial assay, make it ...
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