wheat germ cell-free system
Synthesis of Newcastle disease virus polypeptides in a wheat germ cell-free system.
... The polypeptide molecular weight, 56,000, synthesized in a cell-free reaction, that comigrates with authentic NP has a tryptic peptide pattern identical to the tryptic peptides of NP fro[r] ...
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In Vitro Synthesis of Turnip Yellow Mosaic Virus Coat Protein in a Wheat Germ Cell-Free System
... To analyze the in vitro synthesized products extract was prepared essentially as described by on sodium dodecyl sulfate SDS-polyacrylamide Davies and Kaesberg 5, with a few modifications[r] ...
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Expression of Functional Plasmodium falciparum Enzymes Using a Wheat Germ Cell-Free System
... traditional cell-based expression systems, and the targets of the best new leads for antimalarial drug discovery are ei- ther not known or not available in functional ...of cell-free systems for ...
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Plasmodium vivax: comparison of immunogenicity among proteins expressed in the cell free systems of Escherichia coli and wheat germ by suspension array assays
... and wheat germ cell-free expression systems using commercially available vectors ...the cell- free E. coli system, however, were mostly degraded and showed low amounts of ...
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Protein structural biology using cell-free platform from wheat germ
... the cell-free translation format [26, 27], structural biology primarily requires a supply of specific protein ...other cell-free systems, E. coli cell-free protein production is ...
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N-Terminal Prodomain of Pfs230 Synthesized Using a Cell-Free System Is Sufficient To Induce Complement-Dependent Malaria Transmission-Blocking Activity
... a wheat germ cell- free ...the wheat germ cell-free expressed trun- cated forms of Pfs230C and to define the minimal region of Pfs230 sufficient to induce the ...
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Regulation of Brome Mosaic Virus Gene Expression by Restriction of Initiation of Protein Synthesis
... 330 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest The translation of total and individual brome mosaic virus BMV RNAs was examined in a wheat germ cell-free system in[r] ...
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The phosphorylation of HIV 1 Gag by atypical protein kinase C facilitates viral infectivity by promoting Vpr incorporation into virions
... a wheat germ cell-free protein production system, and screened them for their association with Gag with the amplified luminescent proximity homogenous assay (AlphaScreen) ...a ...
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Expression of a Sheep Pox Virus Gene in Plant Systems under the Control of Plant Viral Regulatory Elements and with Sub-Cellular Targeting
... In this work, we compared several 5'-UTRs of plant viruses and an artificial translational enhancer (ARC1) by their ability to stimulate translation of a downstream ORF. Unlike the leader of the naturally capped AMV ...
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Development of high-yield autofluorescent protein microarrays using hybrid cell-free expression with combined Escherichia coli S30 and wheat germ extracts
... Protein size and domain structure may also influence folding as reflected by the results that we obtained. Eukaryotic cells contain a much greater number of longer proteins than prokaryotes [53] and these proteins ...
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Cell-free translation of influenza virus mRNA.
... the two major translation products obtained in vitro in the wheat germ system programmed with infected cell mRNA to the corresponding proteins M and NP derived from purified virus.. Thes[r] ...
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Evaluation of antibody responses to the early transcribed membrane protein family in Plasmodium vivax
... a wheat germ cell free protein expression system and the purified proteins were used for protein microarray and immunization of ...
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The Effects of Fasting and Feeding on Preproinsulin Messenger RNA in Rats
... The purpose of these experiments was to determine whether alterations in preproinsulin messenger (m)RNA activity could account for changes in insulin biosynthesis during fasting and refeeding. Rats were fasted 4 d and ...
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Wheat and Its Application in Dairy Products: A Review
... Set Yoghurt: Yoghurt is obtained by coagulating milk formed by starter culture consisting of Streptococcus thermophilus and Lactobacillus delbruckii subsp. Bulgaricus. Yoghurt can be classified into set, stirred, and ...
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Csk differentially regulates Src64 during distinct morphological events in Drosophila germ cells
... between germ cells within an individual cyst as well as adhesion between germ cell and follicle cell ...within germ cells to regulate E-cadherin ...at germ cell or ...
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Germ cells and germ cell transplantation
... culture system (McLaren and Buehr, 1990); this transition does not require stimulation from the somatic tissue of the ovary, since oogonia released from the embryonic ovary and transferred to embryonic lung ...
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Nutritional, chemical and microbiological changes during fermentation of tarhana formulated with different flours
... [12]. Wheat gluten proteins are gliadins and glutenins, which are grouped based on their extractabil- ity (gliadins) or unextractability (glutenin) in aqueous al- ...
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In Vitro Translation of the Two RNAs of Nodamura Virus, a Novel Mammalian Virus with a Divided Genome
... These results showed that the RNA 2 product synthesized in wheat germ extracts is indistinguishable from that made in HeLa cell extracts, that p39 is probably an incomplete form of p43, [r] ...
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DNA methylation is a primary mechanism for silencing postmigratory primordial germ cell genes in both germ cell and somatic cell lineages
... Primordial germ cells (PGCs) are the founding population of cells that will ultimately give rise to the mature ...female germ cells entering prophase I of ...
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Microbes, the gut and ankylosing spondylitis
... Macrophages patrol the gastrointestinal systems in high numbers. Th ey frequently come in contact with ‘stray’ bacteria, including commensals that have breached the epithelial cell barrier. Macrophages phagocytose ...
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