Whole cell population versus single cell analysis
Single Cell Proliferation to Produce Monoclonal Population of Transfected Caprine Fibroblast Cell
... the single cell isolation from transfected cells and its proliferation, which include methods of single cell pickup, analysis of media supplements on growth parameters and optimizing ...
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Bayesian Statistical Models of Cell-Cycle Progression at Single-Cell and Population Levels
... as cell density in a field of view increases, the ability to track in- dividual cells and cell lineages ...a single time-lapse movie is much smaller ( 15-30) than the number of cells mon- itored in ...
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Whole population cell analysis of a landmark rich mammalian epithelium reveals multiple elongation mechanisms
... quantify cell ensemble behaviour at a statistical level to link gross tissue changes to single cell and ultimately molecular genetic and epigenetic ...
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Comparative Effectiveness of Acellular Versus Whole-Cell Pertussis Vaccines in Teenagers
... Since late 2005, nearly all pertussis testing in KPNC was done by real-time polymerase chain reaction (PCR) in a single, centralized laboratory. KPNC has exclusively used pertussis PCR kits from Cepheid ...
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From Single Cell to Whole Body: Developing a Molecular Neuroscience Toolkit
... stem cell population with the capacity to self-renew and to differentiate into numerous cell types, the dysregulation of which leads to a multitude of neurocristopathies 17 with extremely detrimental ...
100
Single-Cell Whole Genome Sequencing on the C1 System: a Performance Evaluation
... bulk analysis masks the fundamental features of subclonal variegation of genetic lesions and of clonal ...phylogeny. Single-cell genetic profiling via whole genome sequencing (WGS) provides a ...
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Single-cell analysis of glandular T cell receptors in Sjögren’s syndrome
... T cell clones found in SG of SS subjects are also likely to be frequently found in PB of pSS ...T cell clones from our cohort was very low in PB of 2 healthy ...and single-cell ...
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Single cell transcriptomics suggest that human adipocyte progenitor cells constitute a homogeneous cell population
... unbiased single cell transcriptomics we aimed to characterize ASC populations in human subcutaneous white adipose tissue ...574 single cells from the WAT total stroma vascular fraction (SVF) of four ...
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Proteomics analysis reveals a Th17-prone cell population in presymptomatic graft-versus-host disease
... hematopoietic cell transplantation (HCT), and a blood biomarker that permits stratification of HCT patients according to their risk of developing GI-GVHD would greatly aid treatment ...T cell ...
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Single Cell Analysis of the Physiology of Mechanosensation in Bacteria
... the cell membrane allows these cells to survive an osmotic ...a whole battery of such channels has been discovered with seven different mechanosensitive channels now demonstrated experimentally in ...for ...
157
A compendium of single cell analysis in aging and disease
... purposes. Single-cell expression profiling in atherosclerotic and inflammatory significant vascular cells of smooth muscle cell (SMC) and endothelial cell subpopulations has revealed ...
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Challenges and emerging directions in single cell analysis
... Background Cell-to-cell variation is a universal property of multi- cellular organisms, which contain diverse cell types characterized by different functions, morphologies, and gene expression ...any ...
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Single cell analysis of endothelial morphogenesis in vivo
... or whole growing vessels rather than in individual ECs, which generally cannot be definitively distinguished from their ...identifying single ECs in ...
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Optimized multiplex immunofluorescence single-cell analysis reveals tuft cell heterogeneity
... tuft cell markers. MxIF identified differing profiles of tuft cell expression in the small intestine and the colon and was able to identify changes in the colonic population following introduction of ...
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A genome-wide, single-cell analysis of vascular smooth muscle cell plasticity
... Joint analysis of profiled cells could then reveal whether the phenotypically switching VSMC population is found within both the Myh11-traced and Sca1-traced ...
171
In silico lineage tracing through single cell transcriptomics identifies a neural stem cell population in planarians
... pseudotime analysis on the remaining cells to reveal a putative progression through transient states along a neural ...stem cell and neural markers and showed that they decrease and in- crease, ...
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Single cell sequencing in stem cell biology
... of single-cell omic ...the single-cell omic datasets, such as high technical noise and high false-negative rates, and the pros of these data- sets, such as high sampling numbers and UMIs or ...
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Dissecting population and single-cell heterogeneity in response to anti-cancer drugs
... I would also like to thank my collaborators who contributed to this project, both experimentally and computationally. Experimental methods developed by Jia- Ren Lin and help with the computational analysis ...
103
Development of microfluidic cell growth and stress response analysis platforms at single cell resolution
... the cell attach to the ...the cell growth pattern (Fig ...incremental cell growth. The cell growth graphs belonging to the first and second growth chamber, and the petri dish bulk cell ...
79
Divergent susceptibilities to AAV SaCas9 gRNA vector mediated genome editing in a single cell derived cell population
... focused on potential off-targeted editing on genome loci with high similarities to target sequences [7, 23]. However, this approach assesses a subset of potential off-target sites and could miss a much larger number of ...
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