whole-cell voltage clamp
High glutamate permeability and distal localization of Best1 channel in CA1 hippocampal astrocyte
... of voltage error due to astro- cyte’s leaky membrane in our experimental condition, we next performed a dual patch-clamp recording onto CA1 astrocytes to observe the actual membrane voltage during ...
9
Human neocortical excitability is decreased during anoxia via sodium channel modulation
... Whole-cell voltage-clamp studies of acutely isolated human neocortical pyramidal neurons demonstrate that anoxia and metabolic inhibition produce a large negative shift in the steady-sta[r] ...
9
Histamine induced modulation of olfactory receptor neurones in two species of lobster, Panulirus argus and Homarus americanus
... Whole-cell voltage-clamp recording from the isolated soma of a lobster olfactory receptor cell membrane potential held at —25 mV, normal patch solution: Eci= — 66mV.. Pressure ejection o[r] ...
14
Angiotensin II depolarizes podocytes in the intact glomerulus of the Rat
... Membrane voltage (Vm) and ion currents of pod were examined with the patch clamp technique in fast whole cell and whole cell nystatin ...
11
Characterization of voltage gated ionic currents in a peripheral sensory neuron in larval Drosophila
... Whole cell voltage and current clamp recordings were performed with an Axopatch 1D amplifier (Molecular Devices, Sunnyvale, ...For voltage clamp analysis, leak currents were ...
7
Errors in the measurement of voltage-activated ion channels in cell-attached patch-clamp recordings
... intracellular voltage response, which was linearly related to the amplitude of ensemble HCN1 channel activity, when measured at steady state ...depolarizing voltage responses act to decrease the transmem- ...
9
Reconstruction of cell surface densities of ion pumps, exchangers, and channels from mRNA expression, conductance kinetics, whole cell calcium, and current clamp voltage recordings, with an application to human uterine smooth muscle cells
... Voltage-gated potassium channel hERG. The hERG (the human ether-à-go-go-related gene), alias KCNH2 gene, encodes a protein known as Kv11.1 potassium ion channel, which is voltage-gated with inward ...
64
PubMedCentral-PMC5134327.pdf
... patch clamp electrophysiology would improve the ability of neurochemical investigations to obtain and relate information spread over multiple ...patch clamp and FSCV ...and voltage measurements, a ...
20
The influence of 8-prenylnaringenin on the activity of voltage-gated kv1.3 potassium channels in human jurkat t cells
... the whole-cell patch-clamp technique, we investigated the influence of 8-prenylnaringenin on the activity of the voltage-gated ...lymphocyte cell line ...cancer cell lines ...
12
Purinergic and cholinergic modulation of spinal motor networks in mice
... performing whole-cell patch-clamp recordings of ventral horn ...potential. Voltage- clamp recordings of ventral horn interneurons held at -60mV, revealed that bath application of ...
139
Electrophysiological assessment of primary cortical neurons genetically engineered using iron oxide nanoparticles.
... The development of safe technologies to genetically modify neurons is of high interest in regenerative neurology, for both translational and basic science applications. Such approaches have conventionally been heavily ...
13
Sodium channels enable fast electrical signaling and regulate phagocytosis in the retinal pigment epithelium
... hESC-derived RPE were spontaneously differentiated in floating cell clusters. The pigmented areas were isolated manually and the cells were dissociated with Tryple Se- lect (1X, Thermo Fisher Scientific) and ...
19
Tumor necrosis factor-α enhances voltage-gated Na+ currents in primary culture of mouse cortical neurons
... The activation of many pivotal signal pathways (including p38 MAPK pathway and NF-κB pathway) by TNF-α in the CNS is mediated by TNFR1 [2]. The p38 MAPK signal pathway seemingly has a multiple and contradictory roles in ...
10
Voltage clamp frequency domain analysis of NMDA activated neurons
... of voltage-sensitive membrane parameters to cellular electrical properties it is important to obtain driving point functions of lamprey neurons at different membrane potentials (Moore et ...excitable cell ...
29
Expression of functional inhibitory neurotransmitter transporters GlyT1, GAT-1, and GAT-3 by astrocytes of inferior colliculus and hippocampus
... Using whole- cell patch-clamp recordings from sulforhodamine 101 (SR101)-labeled astrocytes [19, 41, 42] and con- comitant application of glycine or GABA to provoke transporter activation, together ...
16
Developmental changes in spinal neuronal properties, motor network configuration and neuromodulation at free swimming stages of Xenopus tadpoles
... NEW & NOTEWORTHY A novel in vitro brainstem/spinal cord preparation is described that enables whole cell patch-clamp recordings from spinal neurons in pro-metamorphic Xenopus tadpoles.. [r] ...
33
Multiphysics model of a rat ventricular myocyte: A voltage-clamp study
... to build up (phase ‘b’ of Figure 7B-iii) although at a slower rate (compare isometric and isosarcometric traces in Figure 7A), causing a delayed time to peak (84 ms and 120 ms in isosarcometric and isometric cases, ...
27
Modeling CICR in rat ventricular myocytes: voltage clamp studies
... Our voltage clamp simulations have dealt with clamp voltages in the range -30 mV ≤ V ≤ 40 ...larger clamp voltages (40 mV ≤ V ≤ 60 mV) to explore the high voltage behavior of the ...
66
Acid sensing ion channels: potential therapeutic targets for neurologic diseases
... Severe cerebral ischemia may lower brain pH to 6.3 or less, resulting in Ca 2+ overload and neuronal cytotoxicity. ASIC1a and ASIC2a are widely expressed in the central nervous system [56]. Because ASIC1a is selectively ...
8
Taurodeoxycholate activates potassium and chloride conductances via an IP3 mediated release of calcium from intracellular stores in a colonic cell line (T84)
... We used perforated and standard whole-cell patch-clamp techniques, and the intracellular Ca2+ indicator fura-2 to investigate the mechanism of TDC' stimulation of ionic conductances in t[r] ...
10