Whole transcriptome
Whole transcriptome characterization of aberrant splicing events induced by lentiviral vector integrations
... a whole transcriptome analysis of chimeric LV-cellular fusion transcripts in transduced human lymphoblastoid cells and primary hematopoietic stem/progenitor ...
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Whole transcriptome sequencing identifies BCOR internal tandem duplication as a common feature of clear cell sarcoma of the kidney
... All the samples were negative for the presence of YWHAE-NUTM2 fusion transcript from t(10;17) translocation (data not shown). Whole transcriptome massively parallel sequencing was performed on tumor RNA, ...
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<p>Whole transcriptome sequencing identifies crucial genes associated with colon cancer and elucidation of their possible mechanisms of action</p>
... As shown in Figure 4, KIAA0125 and MSTRG.35002.1 were significantly downregulated in both ES and LS colon tumor tissues compared with normal colon tissues, which was in line with the [r] ...
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Whole exome sequencing of a single osteosarcoma case—integrative analysis with whole transcriptome RNA-seq data
... (WES) and whole genome sequencing (WGS) studies, TP53, PTEN and PRB2 are found to be mutated in sig- nificant frequency [5]. High mutation rate in TP53 has also demonstrated in OS cell lines. Additionally, ...
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Transcription factor motif enrichment in whole transcriptome analysis identifies STAT4 and BCL6 as the most prominent binding motif in systemic juvenile idiopathic arthritis
... An additional gene that was highly upregulated in the active stages of sJIA was CD177, confirming recent re- sults [6]. CD177 codes for human neutrophil antigen 2 (HNA-2), also called NB1, a cell surface glycoprotein ...
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p53 Is a Host Cell Regulator during Herpes Simplex Encephalitis
... Next, to investigate the effect of p53 knockout on global viral and host cellular gene expression in the brains of infected mice, we performed whole-transcriptome shotgun sequencing analysis of the brains ...
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Analysis of the Aspergillus nidulans transcriptome using high-throughput RNA sequencing
... of whole transcriptome RNA-seq libraries, as sequences shorter than 50 bp result in a high number of mismatches during alignment and the read being rejected as low ...
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RRM2 is a potential prognostic biomarker with functional significance in glioma
... the whole transcriptome and highly changed genes. GSEA for the whole transcriptome demonstrated that the genes whose expression was elevated upon RRM2 knock-down were significantly enriched ...
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Dual RNA seq reveals viral infections in asthmatic children without respiratory illness which are associated with changes in the airway transcriptome
... polyA-selected whole transcriptome RNA-seq data on the 21 qPCR virus-positive subjects, 2 CCL8/CXCL11-high but viral qPCR-negative subjects, and 25 randomly selected viral qPCR-negative ...the ...
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Comprehensive portrait of recurrent glioblastoma multiforme in molecular and clinical characteristics
... targets for therapy is urgently. To date, the distinction between primary and recurrent GBM has not been systematically investigated. In the present study, 88 primary and 22 recurrent GBM were investigated using ...
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Dysregulated Glycoprotein B-Mediated Cell-Cell Fusion Disrupts Varicella-Zoster Virus and Host Gene Transcription during Infection
... using whole transcriptome sequencing (RNA-seq) to identify viral and cellular responses induced when gBcyt regulation was disrupted by the gB[Y881F] ...
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Transcriptome scale similarities between mouse and human skeletal muscles with normal and myopathic phenotypes
... teristics in a given human muscle type than another. Based on gross histology, skeletal muscles differ in at least four parameters: bulk, length, fiber architecture and fiber type proportions. It is not immediately clear ...
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Role of p53 in pseudorabies virus replication, pathogenicity, and host immune responses
... To investigate the effects of p53 knockout on the host cell gene expression in the brains of the infected mice, we performed a whole-transcriptome shotgun sequencing analysis of the b[r] ...
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The Hidden Genomic and Transcriptomic Plasticity of Giant Marker Chromosomes in Cancer
... (ChIP-seq), whole-genome sequencing (WGS), immunofluorescence in situ hybridization (immu- no-FISH), whole-transcriptome sequencing (total RNA-seq), and other molecular analyses, we investigated in ...
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Activation of the basal cell carcinoma pathway in a patient with CNS HGNET-BCOR diagnosis: consequences for personalized targeted therapy
... RNA transcriptome analysis (RNA- ...heterogeneity, whole-transcriptome sequencing was performed using fresh frozen tissues from two regions of the CNS HGNET-BCOR primary tumor and one inoculation ...
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The evolution of combined molecular targeted therapies to advance the therapeutic efficacy in melanoma: a highlight of vemurafenib and cobimetinib
... using whole-exome sequencing and whole-transcriptome sequencing (RNA-seq) on tumor samples from five patients with acquired resistance to combined dabrafenib and tram- etinib therapy demonstrated ...
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Whose Gene Is It Anyway? The Effect of Preparation Purity on Neutrophil Transcriptome Studies
... Our experiments showed that neutrophil suspensions isolated by Polymorphprep express only very low levels of transcripts that are attributable to contaminating leukocytes. Further- more, cytokine treatment had very ...
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Pankova, Katarina (2017): Neurotransmitters in the neuronal circuit for motion vision in Drosophila melanogaster. Dissertation, LMU München: Graduate School of Systemic Neurosciences (GSN)
... Understanding how neuronal circuits perform computations on the cellular and molecular level is a crucial step towards deciphering how brains function. Yet, the complete elucidation of mechanisms underlying simple ...
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Extreme sensitivity of gene expression in human SH SY5Y neurocytes to ultra low doses of Gelsemium sempervirens
... The homeopathic dilutions/dynamizations were prepared in a manner comparable to methods used by commercial manufacturers, i.e. using 30% ethanol for all dilution/ succussion steps. Since ethanol at higher concentration ...
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Integrated analysis of whole-exome sequencing and transcriptome profiling in males with autism spectrum disorders
... The technological improvements of the last decade have lead to tremendous advances in understanding the genetic basis of ASD, revealing a high degree of genetic heterogen- eity. Clinical application of molecular ...
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