Xenopus egg
DNA replication is required for the checkpoint response to damaged DNA in Xenopus egg extracts
... What might these structures correspond to? In Esche- richia coli , replication forks that are stalled by base lesions are processed in a manner that allows lesion bypass and replication restart, and the biochemistry of ...
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Altering the position of the first horizontal cleavage furrow of the amphibian (Xenopus) egg reduces embryonic survival
... Int I Dc, BioI 36 527 535 (1992) 527 Original Arlie/" Altering the position of the first horizontal cleavage furrow of the amphibian (Xenopus) egg reduces embryonic survival HIROKI YOKOTA', ANTON W NE[.] ...
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Cell free Xenopus egg extracts for studying DNA damage response pathways
... Cell-free Xenopus egg extracts, derived from the eggs of African clawed frogs (Xenopus laevis), have been widely used for studies concerning DDR ...using Xenopus egg extracts to ...
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Intrinsic chiral properties of the Xenopus egg cortex: an early indicator of left right asymmetry?
... The Xenopus egg is cylindrically symmetric until fertilization, when the site of sperm entry superimposes the DV and LR body dimensions onto the original animal-vegetal axis (Manes and Elinson, ...
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Molecular Characterization of the Dbf4/Drf1 Dependent Kinase (DDK) and the DNA Replication Checkpoint Mediator Claspin in Xenopus Egg Extracts
... Genetic analyses in a variety of systems also provided evidence that Cdc7 is a key regulator of DNA replication initiation. Firstly, the phenotype for the null mutant of S. pombe Hsk1 is premature entry into mitosis and ...
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Studying essential DNA metabolism proteins in Xenopus egg extract
... monitored by western blot to identify the stage of DNA replication reaction in which they are involved (Errico and Costanzo, 2010). For these experiments human genomic DNA cloned in the human bacmid library can be used. ...
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Aurora A: an expedition to the pole of the spindle in Xenopus egg extracts
... in Xenopus laevis Aurora-A is sufficient to abolish activation by TPX2 (Eyers et ...In Xenopus laevis egg extract Aurora-A is phosphorylated on Serine 349 and the mutation S349D completely abolished ...
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Calcium requirements during mitotic cdc2 kinase activation and cyclin degradation in Xenopus egg extracts
... Subsequent anaphase sister chromatid separation in both CSF and mitotic extracts can be prevented by competitive inhibi- tion of cyclin degradation (Holloway et al., 1993; Morin et al., 1994). These observations are ...
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The Activation of ATR in Response to Double-Stranded DNA Breaks
... in Xenopus egg extracts and human and mice tissue culture cells showed that even in the absence of damage overexpression of the key regulatory domain of TopBP1, which mediates the association to ATR, the ...
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Regulation of Bovine Papillomavirus Replicative Helicase E1 by the Ubiquitin-Proteasome Pathway
... in egg extract occurs by the ubiquitin-pro- teasome ...the egg extracts with different modified forms of ubiquitin as well as by inhibiting the activity of cellular isopeptidases, which cleave ubiquitin ...
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Reprogramming of somatic cells and nuclei by Xenopus oocyte and egg extracts
... of Xenopus eggs (Ostrup et ...in Xenopus eggs about 30 minutes after activation and continues for approximately 30 minutes (Wangh et ...So, Xenopus eggs and egg extracts were utilized to ...
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Inhibitor-2 induced M-phase arrest in Xenopus cycling egg extracts is dependent on MAPK activation
... There is evidence linking Inh-2 and PP1 under physiological conditions, as Inh-2 protein levels increase during entry into M phase and inhibit PP1 phosphatase activity [31]. PP1 activity has been shown to oscillate ...
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Understanding the role of Claspin in DNA damage checkpoints
... RPA-depleted Xenopus egg extracts, ATR can no longer associate with chromatin and can’t be activated in response to topoisomerase inhibitors (Costanzo and Gautier ...
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New clues to understand how CENP-A maintains centromere identity
... in Xenopus egg extracts is that one can analyze the effect of eliminating one specific factor after a single round of CENP-A loading without accumulation of errors from previous rounds or alteration of cell ...
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Evaluation of the stability of whipped egg white
... Confectionery products made of egg white foam and sugar (e.g. meringue and angel food cake) are popular due to their specific texture properties and good appearance. In some countries, includ- ing Hungary and ...
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The development of free swimming in Xenopus laevis larvae
... Spontaneous activity in developing nervous systems is often due to either the depolarising action of classical inhibitory transmission or unstable membrane potentials due to pacemaker currents or a combination of the two ...
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Proliferative capacity and cytokine production by cells of HIV infected and uninfected adults with different helminth infection phenotypes in South Africa
... the egg + IgE hi when compared to the other three subgroups (Panels B, D and ...both egg positive subgroups (the egg + IgE hi and the egg + IgE lo ) had significantly higher Ki67 proliferation ...
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Performance of Koekoek Chicken Fed with Different Levels of Moringa oleifera Leaf Meal
... external egg quality in the present study were affected positively by the dietary substitution of SBM by MOLM at different levels in dual purpose Koekeok ...weight, egg length, albumen height, yolk index, ...
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Determination of egg yolk content in egg liqueurs
... the egg contents resides in the high variability of the raw material (cholesterol content varies from 840 to 1970 and from 170 to 550 mg per 100 g in yolk and the edible part of egg, respectively) (M������ ...
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Effect of hen age, environmental temperature, and oviposition time on egg shell quality and egg shell and serum mineral contents in laying and broiler breeder hens
... Serum Mg levels decreased at the same rate during the day (–0.045, Table 3) irrespective of treatment, but the content was higher in younger hens. Pavlik et al. (2009) described plasma Mg levels increasing till 47 weeks ...
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