Zinc-binding
Hepatitis B Virus X Protein Function Requires Zinc Binding
... Biophysical analyses of recombinant HBx indicate that C61, C69, C115, and C137 are involved in zinc binding. We next performed mass spectrometry-based hydrogen-deuterium exchange (HDX) analysis of the ...
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Ebola Virus Transcription Activator VP30 Is a Zinc-Binding Protein
... strong zinc-binding capacity of the VP30 peptide (amino acids 68 to 95) when fused to GST under the conditions ...the zinc-binding domain of Sendai virus V protein led to the reduction of ...
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Zinc-binding and protein-protein interactions mediated by the polyomavirus large T antigen zinc finger.
... The zinc blotting is not quantitative, so both H-4653D and H-4723L might have been expected to be positive, given their reduced but signifi- cant ...all zinc binding proteins work in such assays; ...
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Characterization of the Zinc Binding Activity of the Rubella Virus Nonstructural Protease
... a zinc binding motif in several types of ...metal binding site (His1190 and His 1204) were not essential for Zn ...by zinc binding by the imidazole group of histidine and carboxyl ...
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Oral zinc supplementation restore high molecular weight seminal zinc binding protein to normal value in Iraqi infertile men
... weight zinc binding protein (IMW-ZnBP), [because it has molecu- lar weight more than 3000 and less than 80 000], the first Semenogelin I (a protein of molecular weight (MW) 52 kDa) and Semenogelin II ...
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Mutational analysis of a predicted zinc-binding motif in the 26-kilodalton protein encoded by the vaccinia virus A2L gene: correlation of zinc binding with late transcriptional transactivation activity.
... 10 0022-538X/93/105749-05$02.00/0 Copyright © 1993, American Society for Microbiology Mutational Analysis of a Predicted Zinc-Binding Motif in the 26-Kilodalton Protein Encoded by the Va[r] ...
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Native mass spectrometry approaches to study zinc binding plasma proteins
... metal binding. It is likely that Zn 2+ binding modifies the protein structure of HRG in order to mediate interactions with other biomolecules (Jones et ...the binding of Zn 2+ to the HRR is ...
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PubMedCentral-PMC4955873.pdf
... one zinc ion was identi fi ed in the crystal struc- ture of ZE2 (described below), it is unclear where this second binding event occurs; ZE2 contains several patches of acidic residues which could ...
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Molecular characterization of the HIV 1 gagnucleocapsid gene associated with vertical transmission
... two zinc finger like ...critical zinc binding amino acids are mutated to non-zinc binding residues, it results in virions that are defective in RNA packaging and replication ...RNA ...
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Structure-Function Characterization of the Human Dual Specificity Phosphatase hYVH1
... The human orthologue, hYVH1 (also termed DUSP12), shares approximately 30% sequence identity with YVH1 and possesses the characteristic DSP domain and C- terminal zinc-binding domain (Figure 1.4). hYVH1 is ...
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The role of metallothionein in zinc homeostasis
... ‘sense’ zinc levels significantly changed the understanding of zinc ...These zinc-sensing proteins are transcription factors that can alter the expression of zinc transporters and storage ...
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S-nitrosation on zinc finger motif of PARP-1 as a mechanism of DNA repair inhibition by arsenite
... a zinc finger DNA repair protein. The cysteine-rich zinc finger motif serves as a key structure in DNA binding, which is critically important to PARP-1 DNA recognition and DNA repair activity [27, ...
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Amino acid modulation of in vivo intestinal zinc absorption in freshwater rainbow trout
... chelated zinc from dietary zinc-binding constituents such as phytic ...of zinc absorption in fish where the beneficial effects of adding zinc as an amino acid chelate were greater in ...
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Circulatory zinc transport is controlled by distinct interdomain sites on mammalian albumins
... two zinc binding sites with significant affinity besides site A in HSA or ...low zinc concentration, together with the presence of this site in the 5IIU ESA structure, this site appears as a ...
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Reduced Schiff base zinc complexes as proposed models of the active site of the dinuclear zinc enzyme A Aminopeptidase
... Two relatively strong bands appear at 1270-1400 cm -1 for all Schiff bases. These bands can be assigned to phenolic C-O stretching vibrations and O-H deformation vibrations [32]. These two peaks still appear in the ...
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Communication between the zinc and tetrahydrobiopterin binding sites in nitric oxide synthase
... supports binding of 2 with the extra methyl facing to F462; while the density for 3 is weaker than that for 1 or 2, yet enough to support binding of 3 in the shown ...
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Biochemical Activities of Highly Purified, Catalytically Active Human APOBEC3G: Correlation with Antiviral Effect
... reported thus far. Using purified APO3G, we initially com- pared the binding affinity of APO3G to DNA and RNA by EMSA. As shown in Fig. 3A, as the concentration of APO3G was raised an increase in the number of ...
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Zinc transporters in Alzheimer’s disease
... of zinc in normal mamma- lian brain development and physiology [25], it also plays a critical role in the function of the central nervous system ...of zinc in the brain, namely vesicular zinc, ...
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Zinc: A Necessary Ion for Mammalian Sperm
... binding partners in human seminal fluid [62, 63]. Zinc-containing Sperm Proteins 110.. Zinc-containing proteins, commonly known as metalloproteins, are capable of binding one or 111.[r] ...
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Zinc Finger Transcription Factors as Molecular Targets for Nitric Oxide-mediated Immunosuppression: Inhibition of IL-2 Gene Expression in Murine Lymphocytes
... In conclusion, inhibition of the DNA binding activities of transcription factors such as the zinc finger proteins Spl and EGR-1 appears to be a molecular mechanism for the inhibition of [r] ...
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