[PDF] Top 20 2A Protease Is Not a Prerequisite for Poliovirus Replication
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2A Protease Is Not a Prerequisite for Poliovirus Replication
... pOM-EGFP ⌬ P1 was constructed by PCR, subcloning into pBluescript II KS(⫹), and recombination. Briefly, the fragment from nt 1 to nt 735 of pOM1 which had an XhoI restriction site downstream of nt 735 was digested by ... See full document
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Defective RNA replication by poliovirus mutants deficient in 2A protease cleavage activity.
... efficient poliovirus replication. In this study, three point mutations in 2A pro (D38E, Y88L, and Y89L ...mutant poliovirus cDNAs was transcribed and translated in vitro, normal proteolytic ... See full document
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Encapsidation and serial passage of a poliovirus replicon which expresses an inactive 2A proteinase.
... was replication competent when transfected into cells previously infected with vaccinia virus ...a poliovirus replicon which contains the human immunodeficiency virus type 1 (HIV-1) gag gene positioned ... See full document
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Protein 2A is not required for Theiler's virus replication.
... the 2A protein of Theiler’s virus does not have any obvious identity with the 2A proteases of poliovirus and rhinovirus, with protein 2A of hepatitis A virus, or even with the 2A ... See full document
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Rhinovirus 16 2A Protease Affects Nuclear Localization of 3CD during Infection
... vitro protease assays highlight this; PABP and eIF4G are cleaved, respectively, by 3C pro and 2A pro with similar efficiencies under in vitro conditions where accessibility is not hindered, in contrast to ... See full document
11
Enterovirus type 71 2A protease functions as a transcriptional activator in yeast
... its protease activity (Figure 5A) but lost its transcriptional activity (Figure ...enteroviral 2A proteases may transac- tivate some cellular genes to benefit virus ...the replication of ... See full document
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Importance of Arginine 20 of the Swine Vesicular Disease Virus 2A Protease for Activity and Virulence
... genome replication is high (approximately 10 ⫺4 substitutions per nucleotide copied, ...live poliovirus type 3 vaccines contain a subset of viral genomes that contain the wt C at nt 472 in the 5 ⬘ noncoding ... See full document
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Ex Vivo and In Vivo Inhibition of Human Rhinovirus Replication by a New Pseudosubstrate of Viral 2A Protease
... against poliovirus 1 (PV-1; GenBank accession number VO1149) and human entero- virus 71 (HEV-71; GenBank accession number AEF32490) 2A pro- teases (data not ...enteroviral 2A proteases for the LVLQTM ... See full document
14
Aichi Virus 2A Protein Is Involved in Viral RNA Replication
... nonstructural 2A protein varies among vi- ruses in amino acid sequence and ...rhinoviruses, 2A is a protease responsible for cleavage of the polyprotein at its own N terminus (14, ...addition, ... See full document
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Cloning and Expression of Protease 2A from Coxsakievirus B3
... viral replication, 2Apro and 3Cpro have been viewed as excellent targets for developing antiviral drugs [5, ...from poliovirus coxsackievirus B4, Human rhino virus (HRV2) and ... See full document
5
Multiple eIF4GI-Specific Protease Activities Present in Uninfected and Poliovirus-Infected Cells
... Poliovirus (PV), coxsackievirus B (CVB), and human rhino- virus infection of HeLa cells results in a dramatic inhibition of host translation within 1.5 to 3 h postinfection. However, only cap-dependent ... See full document
13
Hijacking Components of the Cellular Secretory Pathway for Replication of Poliovirus RNA
... poliovirus replication complex formation, we studied viral RNA replication in cells ectopically expressing GBF1 and ...virus replication to the downregulation of a particular protein (not ...a ... See full document
10
A poliovirus replicon containing the chloramphenicol acetyltransferase gene can be used to study the replication and encapsidation of poliovirus RNA.
... B A CAT-specific protein with an Mr of 51,000 CAT-VP3 fusion indicated by the arrow is present in extracts of cells transfected with FLC/REP RNA lane 3 but is absent from mock-transfecte[r] ... See full document
7
Evidence for Functional Protein Interactions Required for Poliovirus RNA Replication
... Previous studies from this laboratory were aimed at analyz- ing the viability and growth patterns of chimeric polioviruses in which the N-terminal amphipathic helix in viral protein 2C was replaced by orthologous ... See full document
11
Poliovirus proteinase 2A induces cleavage of eucaryotic initiation factor 4F polypeptide p220.
... To study whether this inhibition of 2A activity has an effect on the cleavage of p220, we translated pMN30 RNA in vitro in the presence of increasing concentrations of anti-2A IgG and in[r] ... See full document
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Limited expression of poliovirus by vaccinia virus recombinants due to inhibition of the vector by proteinase 2A.
... Other lanes: a, P109 immunoprecipitated from cells infected only with VVPO-31; c, wild-type vaccinia virus plus Sabin type 1 poliovirus; e, wild-type vaccinia virus plus Sabin type 2 pol[r] ... See full document
6
A poliovirus minireplicon containing an inactive 2A proteinase is expressed in vaccinia virus-infected cells.
... The transfection of the chimeric HIV-2-gag-poliovirus genome containing the 2A mutation into poliovirus-vaccinia virus coinfected cells resulted in the expression and partial processing [r] ... See full document
9
Expression of the poliovirus receptor in intestinal epithelial cells is not sufficient to permit poliovirus replication in the mouse gut.
... viral replication was detected in the alimentary tract, as determined by titration of virus in the feces and in tissues of the small ...of poliovirus to replicate in the TgFABP-PVR mouse small intestine was ... See full document
6
An Authentic 3′ Noncoding Region Is Necessary for Efficient Poliovirus Replication
... In vitro translation kinetics of in vitro-derived transcript RNAs. It was possible that the addition of an authentic 3 ⬘ - terminal poly(A) did not affect genomic RNA replication di- rectly but rather corrected an ... See full document
12
Cold-adapted poliovirus mutants bypass a postentry replication block.
... FIG. 5. Mapping of the ca mutations. (A) Genomic RNAs of viral recombi- nants between wt and ca viruses are shown schematically, with P1/Mahoney sequences in open boxes and ca virus sequences in black boxes. At the top ... See full document
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