[PDF] Top 20 cis-acting sequences involved in human immunodeficiency virus type 1 RNA packaging.
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cis-acting sequences involved in human immunodeficiency virus type 1 RNA packaging.
... vector RNA expression and ...UTR packaging signal, encapsidation is reduced or abol- ished ...env sequences downstream of the heterolo- gous ...essential cis-acting packaging ... See full document
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Human Immunodeficiency Virus Type 1 Vif Protein Is an Integral Component of an mRNP Complex of Viral RNA and Could Be Involved in the Viral RNA Folding and Packaging Process
... acids 1 to 64, which is located in the N terminus of the Vif, contains a strong RNA binding ...the RNA binding ...weak RNA binding activity ...heterogeneous sequences, yet they can ... See full document
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Mechanisms of Human Immunodeficiency Virus Type 2 RNA Packaging: Efficient trans Packaging and Selection of RNA Copackaging Partners
... Human immunodeficiency virus type 2 (HIV-2) has been reported to have a distinct RNA packaging mechanism, referred to as cis packaging, in which Gag proteins ... See full document
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Nucleocapsid and Matrix Protein Contributions to Selective Human Immunodeficiency Virus Type 1 Genomic RNA Packaging
... genomic RNA packaging, and some evidence has implicated the matrix protein (MA) of certain retroviruses in viral RNA ...viral RNA and to address the potential contribution of MA in this ... See full document
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RNA Structure and Packaging Signals in the 5′ Leader Region of the Human Immunodeficiency Virus Type 1 Genome
... target RNA into HIV-1 virions ...mous packaging activity spans the first 350 to 400 contiguous bases of the genome, and thus includes not only SL1 to SL4 but also 242 bases upstream (22, 25, 31, ... See full document
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The sequences of and distance between two cis-acting signals determine the efficiency of ribosomal frameshifting in human immunodeficiency virus type 1 and human T-cell leukemia virus type II in vivo.
... stem-loop AG frameshift efficiency % kcal/mol l _______________________ Slippery HIV-1 wild-type 3.17 sequence SlIppery o [071 HTLV-2 Expression vectors for quantitating in vivo translat[r] ... See full document
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Effects of Mutations in the Human Immunodeficiency Virus Type 1 gag Gene on RNA Packaging and Recombination
... The cis-packaging hypothe- sis implies that mutants that cannot generate functional Gag are selected against at two levels: these mutants do not gener- ate infectious virus, and these mutant RNAs ... See full document
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Sequences Downstream of the 5′ Splice Donor Site Are Required for both Packaging and Dimerization of Human Immunodeficiency Virus Type 1 RNA
... viral RNA within the cytoplasm ...viral RNA expressed within the cells revealed that all mutants tested gen- erated approximately twice as much genomic as spliced viral RNA ...viral RNA ... See full document
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Nonreciprocal Packaging of Human Immunodeficiency Virus Type 1 and Type 2 RNA: a Possible Role for the p2 Domain of Gag in RNA Encapsidation
... of human immunodeficiency virus types 1 (HIV-1) and 2 (HIV-2) to cross-package each other’s RNA was investigated by cotransfecting helper virus constructs with vectors ... See full document
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The Human Immunodeficiency Virus Type 1 TAR RNA Upper Stem-Loop Plays Distinct Roles in Reverse Transcription and RNA Packaging
... each virus relative to that of the wild ...wild- type amounts of negative strong-stop DNA in NERT assays ...of sequences from position ⫹22 to ⫹24 had only minor effects on reverse transcription, ... See full document
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A dual role of the putative RNA dimerization initiation site of human immunodeficiency virus type 1 in genomic RNA packaging and proviral DNA synthesis.
... on virus infectivity than loop sub- ...decreased virus infec- tivity by a factor of 10 to 100, indicating that the loop has a biological ...genomic RNA dimer formation and/or stability ...for ... See full document
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An Intact TAR Element and Cytoplasmic Localization Are Necessary for Efficient Packaging of Human Immunodeficiency Virus Type 1 Genomic RNA
... of RNA into particles as an endpoint are subject to several general ...identified packaging element could be a unique domain for the binding of a host or viral factor that somehow mediates the encapsi- ... See full document
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Redundant Roles for Nucleocapsid and Matrix RNA-Binding Sequences in Human Immunodeficiency Virus Type 1 Assembly
... two RNA-binding do- mains in Gag is required for assembly of HIV-1 ...the RNA-binding region of MA or deletion of NC alone does not dramatically reduce virion production per ...these RNA- ... See full document
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cis-acting elements in human immunodeficiency virus type 1 RNAs direct viral transcripts to distinct intranuclear locations.
... detect RNA by hybridization as de- scribed by Johnson et ...mixed 1:1 with hybridization buffer (4 3 SSC [1 3 SSC is ...min, 1 3 SSC at RT for 20 min, and 4 3 SSC at RT for 10 ...to ... See full document
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trans-acting proteins involved in RNA encapsidation and viral assembly in human immunodeficiency virus type 1.
... permitted RNA encapsidation in COS cells, suggesting that the C-terminal zinc finger is dispensable in this cell type, as was suggested in the domain exchange studies of Gorelick et ...viral RNA ... See full document
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Concerted Action of Multiple cis-Acting Sequences Is Required for Rev Dependence of Late Human Immunodeficiency Virus Type 1 Gene Expression
... and 7), confirming previous LMB inhibitor studies (1, 3, 11, 36). However, we also consistently observed reduced levels of nuclear wild-type gag transcripts (Fig. 5B; compare lanes 6 and 8). This ... See full document
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Mutations of RNA and protein sequences involved in human immunodeficiency virus type 1 packaging result in production of noninfectious virus.
... Cells transfected with the mutant genomes, while expressing normal levels of human immunodeficiency virus type 1 RNA and proteins, produced viral particles that were normal in protein co[r] ... See full document
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Mutational analysis of cis-acting packaging signals in human immunodeficiency virus type 1 RNA.
... To determine whether sequences that we had previously demonstrated to be important for binding of HIV-1 RNA to the HIV-1 Gag polyprotein in vitro 9, 35 were necessary for packaging of RN[r] ... See full document
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Packaging of human immunodeficiency virus type 1 RNA requires cis-acting sequences outside the 5' leader region.
... A 1.1-kb env gene fragment encompassing the Rev-responsive element was absolutely required for the expression and encapsidation of vectors containing cis-acting repressive sequences and [r] ... See full document
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Analysis in human immunodeficiency virus type 1 vectors of cis-acting sequences that affect gene transfer into human lymphocytes.
... 3888-3895 0022-538X/94/$04.00 + 0 Copyright © 1994, American Society for Microbiology Analysis in Human Immunodeficiency Virus Type 1 Vectors of cis-Acting Sequences That Affect Gene Tra[r] ... See full document
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