[PDF] Top 20 Activity of the Bacillus anthracis20 kDa protective antigen component

... and found a decrease in CD38-associated fluorescence (Figure 12). Discussion and conclusion It is widely accepted that B. anthracis toxins contribute to anthrax pathogenesis and to date, only the combination of the PA 63 ... See full document

... infection. Protective antigen (PA) from Bacillus anthracis is a potent immunogen, which has been of interest in the development of new candidate vaccines against the ...this antigen on ... See full document

... 63 kDa fragment o f PA may not be a suitable candidate for expression, but other fragments might be both stably expressed and ...immunogenic. Protective epitopes have not yet been identified for PA and so ... See full document

... the activity of anthrax toxin is based on in vitro systems, which delineate the interaction between Bacillus anthracis toxins and the cell ...anthracis protective anti- gen (PA) along with mass ... See full document

... In summary, we evaluated the immunogenicities and protec- tive efficacies of the different domains of PA in different mouse strains. Our results suggest that some mouse strains generate a more potent neutralizing ... See full document

... anthrax protective antigen (PA) is the central component of the three-part anthrax toxin, and it is the primary immunogenic component in the approved AVA anthrax vaccine and the ... See full document

... Conformational changes of chimeric PA/HA proteins. Cell surface expression and HA1-HA2 cleavage do not dictate that the chimeric proteins will be functional. For HA, a useful analytical tool involves experiments on the ... See full document

... neutralizing activity of anti-PA antibody in serum ...tralizing activity of MDX-1303 in that particular sample is at least as good as that obtained using the CDC reference stan- ... See full document

... anthrax protective antigen PA14 domain (InterPro family IPR011658) and a bacterial Binary_ToxB exotoxin domain (InterPro family IPR003896) commonly found in binary Vip1 and other related bacterial toxins ... See full document

... anthrax protective antigen PA14 domain (InterPro family IPR011658) and a bacterial Binary_ToxB exotoxin domain (InterPro family IPR003896) commonly found in binary Vip1 and other related bacterial toxins ... See full document

... of Bacillus anthracis Joseph A. Bellanti, b * Feng-Ying C. Lin, a * Chiayung Chu, a Joseph Shiloach, c Stephen H. Leppla, d German A. Benavides, b Arthur Karpas, a Mahtab Moayeri, d Chunyan Guo, a John B. Robbins, ... See full document

... Discussion In this work, we provide for the first time evidence for a role of Rsk in HD. Within Rsk family, we analyzed Rsk1 and Rsk2, two isoforms that are broadly expressed in the brain, including the striatum, and ... See full document

... encoding the PA component of the anthrax toxin [11]. The host B brevis 47-5Q was cultured form modified PY medium containing proteose peptone (Becton Dickinson, Sparks, MD, USA) 1%, yeast extract (Becton ... See full document

... several molecules with the ability to bind a small cavity (Figure 1, panel B) in the protective antigen located at the oligomerization interface, close to the region where the N-terminal domain contacts the ... See full document

... a protective anti- gen in addition to type 18 M ...streptococcal protective antigen) that evoked antibodies that opsonized the M18 parent and the M-negative ...of protective epitopes within ... See full document

... If the PA-c-Myc protein uses the 9E10 antibody to enter and kill hybridoma cells, the addition of the c-Myc peptide should compete with PA-c- Myc binding and thus protect cells from toxi[r] ... See full document

... for antigen purification to be used as candidate ...fusion, antigen in saline was injected into the tail vain and then mice were killed and the spleen lymphocytes were fused with myeloma ... See full document

... We developed an antigen capture enzyme-linked immunosorbent assay (ELISA) which does not require purified protective antigen (PA) for detection of human antibodies to Bacillus anthracis [r] ... See full document

... of protective immunity against a number of toxin-mediated bacterial diseases, including anthrax; however, the cellular mechanisms that lead to optimal neutralizing antibody re- sponses remain ill ...thrax ... See full document

... VSV-neutralizing IgG was determined after inactivation of IgM by 2-mer- captoethanol (2-ME) (41). Despite controversies over the specificity of this procedure it remains the only method for determining virus neutralizing ... See full document