[PDF] Top 20 Adenovirus-Associated Virus Multiplication
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Adenovirus-Associated Virus Multiplication
... The base composition of each strand species was determined, and it was found that the species banding at a greater density in CsCI heavy strands had an expected higher thymidine content [r] ... See full document
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Adenovirus-Associated Virus Multiplication VIII. Analysis of In Vivo Transcription Induced by Complete or Partial Helper Viruses
... [For simplicity when referring to AAV systems using different helper viruses, we use the following nomenclature in which the helper virus is represented in parentheses: AAVAd refers to A[r] ... See full document
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Adenovirus-Associated Virus Multiplication IX. Extent of Transcription of the Viral Genome In Vivo
... Because the complementary strands of AAV DNA can be separated preparatively 5, it was possible to demonstrate that AAV RNA synthesized in the presence of either a complete or partial hel[r] ... See full document
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Adenovirus-Associated Virus Multiplication VII. Helper Requirement for Viral Deoxyribonucleic Acid and Ribonucleic Acid Synthesis
... On the other hand, a synthesis of AAV deoxyribonucleic acid DNA or ribonucleic acid RNA was not detected by nucleic acid hybridization after KB cells were infected with AAV type 2 alone,[r] ... See full document
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Integrating Adenovirus–Adeno-Associated Virus Hybrid Vectors Devoid of All Viral Genes
... To confirm the integrated status of ⌬Ad.AAV1 DNA, after 4 weeks of selection high-molecular-weight chromosomal DNA from pools of transduced cells was separated by PFGE, followed by Southern analysis with a SEAP-specific ... See full document
11
Production of High-Titer Recombinant Adeno-Associated Virus Vectors in the Absence of Helper Adenovirus
... To overcome these obstacles, we have constructed various Ad miniplasmids to substitute for the Ad virion DNA compo- nent (9). Substituting Ad helper functions from virus to plas- mid DNA for rAAV production has ... See full document
9
Cellular Proteins Required for Adeno-Associated Virus DNA Replication in the Absence of Adenovirus Coinfection
... adeno-associated virus (AAV) DNA replication ...adeno- virus (Ad)-infected HeLa cell cytoplasmic extract to catalyze origin of replication-dependent AAV DNA repli- ...helper virus in cells ... See full document
11
Role of the Adenovirus DNA-Binding Protein in In Vitro Adeno-Associated Virus DNA Replication
... was associated with an increase in proces- sivity as demonstrated by MboI susceptibility and the dis- appearance of short replication products characteristic of displacement of the elongating strand from the ... See full document
8
Efficient Generation and Amplification of High-Capacity Adeno-Associated Virus/Adenovirus Hybrid Vectors
... sarcoma virus (RSV) promoter, the bacteriophage P1 cre gene, and the encephalomyocarditis virus internal ribosome entry site (IRES), followed by the puromycin resistance gene from Streptomyces albondiger ... See full document
11
Identification of an Adeno-Associated Virus Rep Protein Binding Site in the Adenovirus E2a Promoter
... 19. Adenovirus (Ad) is the most efficient helper for AAV, but human papillomavirus (HPV), cytomegalovirus (CMV), vaccinia virus, Epstein-Barr virus, and herpes simplex virus (HSV) can provide ... See full document
11
Adenovirus helper function for growth of adeno-associated virus: effect of temperature-sensitive mutations in adenovirus early gene region 2.
... Two mutants of Ad type 5 Ad5, Ad5ts125 and Ad5tslO7, with temperature-sensitive lesions in the E72 DNA-binding protein coded by the Ad early region 2, were deficient for AAV helper funct[r] ... See full document
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Evidence for a Single-Stranded Adenovirus-Associated Virus Genome: Isolation and Separation of Complementary Single Strands
... These results could be attributed to the fact that the peak of the light band is close to the density of the original doublestranded 3H-BUDR DNA so that the light band of single polynucl[r] ... See full document
7
Adeno-Associated Virus RNAs Appear in a Temporal Order and Their Splicing Is Stimulated during Coinfection with Adenovirus
... adeno-associated virus type 2 (AAV- 2) is a single-stranded DNA virus which requires coinfection of helper virus for establishment of a productive infection (see reference 1 for a ...helper ... See full document
11
Phosphorylation of chromatin- and ribosome-associated proteins in cells transformed by adenovirus, murine sarcoma virus, and methylcholanthrene.
... The high level of endogenous protein phosphorylation in chromatin and ribosomes of various transformed cells is not due to the high cell density because the highly phosphorylated 90K pro[r] ... See full document
9
Transcripts of the adenovirus-associated virus genome: multiple polyadenylated RNAs including a potential primary transcript.
... On the basis of previous demonstrations of the exonuclease VII insensitivity of looped-out DNA regions in DNA-RNA hybrids 3 even when the DNA loop is separated from the RNA terminus by a[r] ... See full document
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Effect of deletions in adenovirus early region 1 genes upon replication of adeno-associated virus.
... Mutants having extensive deletions of adenovirus early region la d1312 or regions la and lb d1313 helped AAV as efficiently as wild-type adenovirus in 293 cells, but neither mutant helpe[r] ... See full document
9
Overexpression of Cyclin A Inhibits Augmentation of Recombinant Adeno-Associated Virus Transduction by the Adenovirus E4orf6 Protein
... The 34-kDa product of adenovirus E4 region open reading frame 6 (E4orf6) dramatically enhances trans- duction by recombinant adeno-associated virus vectors (rAAV). This is achieved by promoting the ... See full document
10
Role of the apical stem in maintaining the structure and function of adenovirus virus-associated RNA.
... Examination of the secondary structure of VA RNA, in solution by using the nuclease sensitivity technique suggests that it contains two stems, the apical stem and the terminal stem, sepa[r] ... See full document
9
A MicroRNA Derived from Adenovirus Virus-Associated RNAII Promotes Virus Infection via Posttranscriptional Gene Silencing
... fection. Adenovirus (Ad) expresses miRNAs derived from VA-RNAs (mivaRNAI and -II); however, recent studies have revealed that processing of VA-RNAI into mivaRNAI in- hibits Ad ... See full document
17
Permanent cell lines that show temperature-dependent expression of adenovirus virus-associated RNA.
... Cultures of E540 and EVA1, 2, and 26 cell lines were incubated at a permissive or a restrictive temperature, fixed, and processed for in situ hybridization to a VAspecific riboprobe as d[r] ... See full document
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