[PDF] Top 20 Advances in the detection of telomerase activity using isothermal amplification
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Advances in the detection of telomerase activity using isothermal amplification
... of telomerase activity in living ...of telomerase activity [74]. Without telomerase, MSN probe was sealed by the wrapping DNA (O1, consists of TS primer and the telomeric repeats), ... See full document
16
Development of a rapid loop mediated isothermal amplification assay for diagnosis and assessment of cure of Leishmania infection
... years advances in the polymerase chain reaction (PCR) based methods have been intro- duced for diagnosis of leishmaniasis [12 – ...for detection of Leish- mania parasite, such as triplex PCR [16], multiplex ... See full document
9
Loop Mediated Isothermal Amplification Method for Rapid Detection of the Periodontopathic Bacteria Porphyromonas gingivalis, Tannerella forsythia, and Treponema denticola
... ated isothermal amplification (LAMP), which constitutes a novel nucleic acid amplification method (28, ...displacement activity and a set of four specially designed primers, termed inner and ... See full document
7
Roles of telomeres and telomerase in cancer, and advances in telomerase-targeted therapies
... of telomerase has significantly improved our under- standing of the role of telomerase in cancer ...of telomerase reactivation in cancer cells, much remains to be learned about the sufficiency or ... See full document
18
Detection of Pathogen-Specific Antibodies by Loop-Mediated Isothermal Amplification
... To construct the LAMPole fusion, we needed to design (i) the sequence of the DNA element and primer sets that specifically recognize this template, even in the presence of contaminating human or pathogen DNA, and (ii) a ... See full document
7
Detection of virus mRNA within infected host cells using an isothermal nucleic acid amplification assay: marine cyanophage gene expression within Synechococcus sp
... tion using a further probe linked to alkaline phosphatase ...phosphatase activity was measured using a plate reader (Labsystems integrated EIA Manage- ment system, Thermo Life Sciences) pre-warmed at ... See full document
8
<p>Loop-mediated isothermal amplification for detection of <em>Legionella pneumophila</em> in respiratory specimens of hospitalized patients in Ahvaz, southwest Iran</p>
... Optimization of the LAMP assay was performed in a 25 µL reaction mixture containing 2.5 µL 10 × Bst buffer (New Eng- land Biolabs, Ipswich, MA, USA), 0.2 pmol each of F3 and B3, 0.8 pmol each of FIP and BIP, different ... See full document
6
Loop mediated isothermal amplification for the detection of goose circovirus
... modulating activity was also presented in the context of the co-infection of geese with West Nile Virus in Hungarian geese flock ...the detection of GCV and other circoviruses of ...simple detection ... See full document
11
Loop Mediated Isothermal Amplification for Detection of African Trypanosomes
... Because we wished to compare the sensitivity of the LAMP and PCR methods targeted to the same gene, we intentionally used PCR primers A1-F3 and A1-B3. It has been shown that microscopy can detect ⬍1,000 parasites in ... See full document
8
Development of a loop-mediated isothermal amplification assay for rapid detection of Yersinia enterocolitica via targeting a conserved locus
... strand-displacement activity of Bst polymerase to improve ...The detection limit of LAMP assay was determined to be 44 copy number, which was 10-fold more sensi- tive than the conventional PCR using ... See full document
6
Detection of Four Plasmodium Species by Genus and Species Specific Loop Mediated Isothermal Amplification for Clinical Diagnosis
... detectable amplification caused by the lower template copy number makes the enzyme activity lower and (ii) the lower template DNA concentration, which decreases the annealing efficiency of the primers, may ... See full document
8
Feasibility and Operational Performance of Tuberculosis Detection by Loop Mediated Isothermal Amplification Platform in Decentralized Settings: Results from a Multicenter Study
... Sample flow and blinding. The two sputa per patient were random- ized: one underwent direct smear examination and a TB-LAMP test while the other underwent smear microscopy only. TB-LAMP and smear oper- ators were blinded ... See full document
8
Loop Mediated Isothermal Amplification (LAMP) Assay for GMO on: Recent Progresses and Future Perspectives
... GMO detection, especially as a field detection al- ternative in scenes where a fast screen is needed while it lacks complex laboratory ...GMO detection in the laboratory and also on spot, if it is ... See full document
9
A loop mediated isothermal amplification assay for the detection and quantification of JC polyomavirus in cerebrospinal fluid: a diagnostic and clinical management tool and technique for progressive multifocal leukoencephalopathy
... fluorescence detection of the LAMP products was also conducted by the addition of dye to each ...this detection method enables the differentiation of positive from negative ... See full document
11
Loop Mediated Isothermal Amplification for Rapid and Reliable Diagnosis of Tuberculous Meningitis
... (Genei Amplification kit; Bangalore Genei, Banglore, ...amplified using specific external ...The amplification products were separated on 2% agarose gels, visualized on a Gel Doc system (Biotech ... See full document
5
Detection of Acanthamoeba and Toxoplasma in River Water Samples by Molecular Methods in Iran.
... mediated isothermal amplification (LAMP) had been utilized for a broad spectrum of applications in the biomedical field including the detection of vi- ruses, bacteria, fungi and parasites (15, ...the ... See full document
8
Rapid and Sensitive Detection of H7N9 Avian Influenza Virus by Use of Reverse Transcription–Loop Mediated Isothermal Amplification
... (AIV) detection in migratory birds and poultry be stringent ...on-site detection or laboratories with poor conditions, especially in developing ...the detection of the new H7N9 influenza ... See full document
5
Development and evaluation of a Loop Mediated Isothermal Amplification (LAMP) technique for the detection of hookworm (Necator americanus) infection in fecal samples
... mediated isothermal amplification technique (LAMP) is a technique developed by Notomi et ...under isothermal conditions. This nucleic acid amplification technique uses only one enzyme ( Bst ... See full document
7
Rolling Circle Amplification on Biotin Streptavidin Complexes Immobilized to Activated Cyclic Polyolefin Surfaces
... Cy5-labeled detection oligonu- cleotide, D5, complementary to the L-RCA product (Figures 2(a) and (b)) was injected into the wells and the chips were incubated in a DNA Engine Peltier Thermal Cycler at 95˚C for 30 ... See full document
11
Isothermal Recombinase Polymerase amplification (RPA) of Schistosoma haematobium DNA and oligochromatographic lateral flow detection
... of detection observed at 100 fg of DNA ...reduced amplification at lower temperatures and no amplification at 50 °C ...2a). Amplification was detectable after as little as 5 min of incubation ... See full document
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