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Amplification mediated by polyomavirus large T antigen defective in replication.
... To further address the role of the replicative function of large T-Ag in amplification, we investigated the effect of polyomavirus mutants in another cell line, designated Hy2, in which [r] ... See full document
5
High-frequency recombination mediated by polyomavirus large T antigen defective in replication.
... We show here that in another cell line, designated Hy5, polyomavirus large T-Ag can promote different recombination events independently of its replicative function and that recombinatio[r] ... See full document
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The Minimum Replication Origin of Merkel Cell Polyomavirus Has a Unique Large T-Antigen Loading Architecture and Requires Small T-Antigen Expression for Optimal Replication
... DNA replication. Several studies using SV40, Py and JCV sT defective viruses and constructs demonstrate an auxiliary role for sT in origin replication (8, 9, ...origin replication activity of ... See full document
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Asp-286----Asn-286 in polyomavirus large T antigen relaxes the specificity of binding to the polyomavirus origin.
... To determine whether binding of large T antigen was responsible for the defective replication of these DNAs and, more generally, to show that binding of large T antigen to the origin was[r] ... See full document
8
Strategy for eliciting antigen-specific CD8+ T cell-mediated immune response against a cryptic CTL epitope of merkel cell polyomavirus large T antigen
... LT antigen contains a premature stop codon that leads to the loss of helicase located at the C-terminus, which is necessary for viral replication ...viral replication ensures the infected cell is ... See full document
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Polyomavirus Large T Antigen Binds Cooperatively to Its Multiple Binding Sites in the Viral Origin of DNA Replication
... by large T antigen to specific DNA, from a maximum at pH 6 to nearly no detectable binding at pH ...either polyomavirus or simian virus 40 large T antigen have been ...of ... See full document
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Enhancer-mediated role for polyomavirus middle T/small T in DNA replication.
... middle T signalling (58, 60), and the activation of these two transcrip- tional activators has a synergistic effect, at least at the tran- scriptional level ...in polyomavirus gene expression and ... See full document
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The Retinoblastoma Protein Alters the Phosphorylation State of Polyomavirus Large T Antigen in Murine Cell Extracts and Inhibits Polyomavirus Origin DNA Replication
... viral replication in herpesvirus- infected ...SV40 T Ag, prevents assembly of T Ag hexamers at the core origin and also interferes with the interaction of T Ag with DNA polymerase ...Py ... See full document
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Zinc-binding and protein-protein interactions mediated by the polyomavirus large T antigen zinc finger.
... pLTR880 (a gift from T. Roberts, Dana-Farber Cancer Institute) expresses LT from a cDNA. The vector consists of pBR322 sequences, the Harvey sarcoma- virus long terminal repeat for transcription initiation, and an ... See full document
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The p110α Isoform of Phosphatidylinositol 3-Kinase Is Essential for Polyomavirus Middle T Antigen-Mediated Transformation
... acid large T antigen (LT) is a nuclear protein with origin- specific DNA binding properties and an ATPase activity es- sential for viral replication ...Small t antigen (st) is a ... See full document
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AP1 enhances polyomavirus DNA replication by promoting T-antigen-mediated unwinding of DNA.
... that replication-compe- tent wild-type helpers compete for replication of test DNAs, indicating that one or more factors limit replication in vivo (41, ...such replication assays is the viral ... See full document
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Elements of the polyomavirus replication origin required for homologous recombination mediated by large T antigen.
... and replication. To verify this possibility, we tested the replication capacity of plasmid pD(a1b)s ...affect replication, presumably because of their separation from ...the T-ag initiation ... See full document
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Multimerization of polyomavirus middle-T antigen.
... the T-antigen mutant dl8; we therefore used it to determine whether 14-3-3 association is essential for dimerization of middle-T antigen ...middle-T antigen by PAb 722 in singly ... See full document
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Polyomavirus tumor induction in mice: effects of polymorphisms of VP1 and large T antigen.
... 335 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest By testing recombinants between "high tumor" inducing a high incidence of tumors and "low tumor" inducing a low inci[r] ... See full document
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Determination of the origin-specific DNA-binding domain of polyomavirus large T antigen.
... Fusion proteins of large T antigen lacking the first 200 N-terminal amino acids bound specifically to polyomavirus origin DNA; however, deletions beyond this site resulted in unstable pr[r] ... See full document
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J Domain-Independent Regulation of the Rb Family by Polyomavirus Large T Antigen
... After serum withdrawal, LT activation of the cyclin A pro- moter, like induction of apoptosis, requires an intact Rb bind- ing site but not J function. In considering possible J-indepen- dent targets for LT, the cyclin A ... See full document
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Transformation and tumorigenic properties of a mutant polyomavirus containing a middle T antigen defective in Shc binding.
... middle T antigen, a mutation that changes the tyrosine at 315 to phenylalanine was introduced into the virus ...middle T that cannot be phosphorylated at this site and thus cannot bind PI 3-kinase ... See full document
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Constitutive expression of simian virus 40 large T antigen in monkey cells activates their capacity to support polyomavirus replication.
... Similar results were observed with three different DNAs containing wild-type polyomavirus origins, and we have observed replication of polyomavirus DNAs in two other types of monkey cell[r] ... See full document
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Polyomavirus Large T Antigen Binds Symmetrical Repeats at the Viral Origin in an Asymmetrical Manner
... of replication (8, 30, ...rus replication proceeds via a bidirectional ...rolling-circle replication is well documented for Py (34) and SV40 (35), as well as for papillomavirus 16, which encodes E1, ... See full document
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Polyomavirus large T antigen binds independently to multiple, unique regions on the viral genome.
... To test the applicability of this method for measuring the binding of polyomavirus large T antigen to specific viral DNA fragments, we incubated increasing amounts of nuclear extract fro[r] ... See full document
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