[PDF] Top 20 Analysis of the Assembly Function of the Human Immunodeficiency Virus Type 1 Gag Protein Nucleocapsid Domain

... the gag gene, causing the gag ORF to terminate 11 residues after (TARK), precisely at (TAM), or 5 residues before (ApoTE) the junction of p2 and ...the gag coding sequences of these constructs, were ... See full document

... precursor Gag (PrGag) pro- teins of retroviruses such as human immunodeficiency virus (HIV), Rous sarcoma virus (RSV), and murine leukemia vi- ruses are sufficient for the production of ... See full document

... The assembly experiments were performed exactly as de- scribed previously (6), except that in some experiments the assembly reaction was scaled up to contain twice the amounts of all constituents in twice ... See full document

... group participates in a hydrogen bond with a residue in NC. As part of our characterization of the binding of NC to oligodeoxynucleotides containing alternating T’s and G’s, we attempted to identify the minimum length ... See full document

... the Gag-Flag fusion protein, Western blotting was carried out using an anti-Flag mouse monoclonal antibody ...of Gag VLPs in ...full-length gag gene of HIV-1, HIV-2, SIVmac, SIVagm, or ... See full document

... Recently, a gel mobility shift assay has been used to measure in vitro binding of purified bovine leukemia virus BLV gag proteins to BLV genomic RNA segments; in this report, NC was show[r] ... See full document

... the Gag double mutant, we inserted potential RNA-binding sequences into the NC deletion region of the MX/NX/PX ...coma virus (RSV) Gag allowed it to bind EF1- ␣ in vitro (13), presumably due to ... See full document

... produce Gag VLP from the cell surface (4, 16, ...in Gag-Gag interaction, however, as non- myristoylated Gag protein may be rescued into budding Gag VLP by a relatively low level ... See full document

... NaCl, 1 mM EDTA, ...NaCl, 1 mM EDTA, 0.1% sodium dodecyl sulfate [SDS], 0.5% sodium deoxycholate, 1% Triton X-100, ...in 1 ml of PBS for each 10-cm-diameter ...in 1 ml of IPB plus ... See full document

... of human immunodeficiency virus (HIV) gag mutants by progressive truncation of the gag coding sequence from the C terminus and have combined these mutants with an ... See full document

... HIV-1 Gag and actin from in- fected cells with a monoclonal antibody against ...HIV-1 Gag molecules could bind actin in infected cells, we performed coimmunoprecipitation exper- ...with ... See full document

... the Gag protein is required for proper formation of HIV-1 cores, which is essential for a productive RT reaction for viral infectivity ...the Gag processing by A1752 could also induce an ... See full document

... and Gag-green fluorescent protein (GFP) fusion proteins that define the HIV-1 I domain have been previously described (30, ...vaccinia virus/T7 poly- merase hybrid expression system was ... See full document

... tant Gag precursors that lacked the C-terminal p6 domain or p6 plus the p1 interdomain ...these Gag processing inter- mediates appeared selectively enriched in the particulate frac- ...only ... See full document

... structs. Virus produced from construct pHX/MT-NC packaged approximately half as much unspliced viral RNA as did the wild type, and it packaged spliced RNA at levels equivalent to unspliced RNA ...NC ... See full document

... to Gag, eventu- ally resulting in an inhibition of virus ...on virus production. Interestingly, expression of HIV-1 ⌿ as an exogenous transcript resulted in a significant inhibition to ... See full document

... WT Gag. The immunofluorescence analysis pre- sented above suggested that the LL189,190AA mutant may be defective for Gag ...mutant Gag to be rescued upon coexpression with WT ...assay, ... See full document

... p6 domain, which is found only in primate lentiviruses, facilitates the separation of virus particles from the cell (17, 25, ...viral protein Vpr (30, 35, 43) and has also been implicated in the ... See full document

... of Gag multimerization in membrane ...of Gag in which protein-protein interactions bring together two or more ...of Gag molecules that are larger than ...HIV-1 Gag. The ... See full document

... simian virus 40-based vector which contains full-length wild-type HIV-1 proviral DNA (23, 45) and was a gift of ...Fig. 1) were verified by direct sequence analysis of the SpeI-SalI ... See full document