[PDF] Top 20 Analysis of Human Immunodeficiency Virus Type 1 Gag Dimerization-Induced Assembly

... which dimerization is ...initiate assembly via a cross-link mechanism. Indeed, assembly initia- tion of CASP1Cys proteins but not CASP1 proteins could be accomplished with the cysteine-specific ... See full document

... A complication in genetically blocking protease cleavage is the difficulty in predicting what sequence substitutions at the protease cleavage site will eliminate protease recognition in the viral mutants. General ... See full document

... in Gag myristylation and that this defect is corrected in the 4AN/6VI double ...that Gag (or MA) assembles into a two- dimensional lattice composed of interlocking six-membered rings, in which three trimers ... See full document

... monkey virus (M-PMV) Gag protein possesses the ability to assemble into an immature capsid when synthesized in a reticulocyte lysate translation ...the human immunodeficiency virus ... See full document

... and 1 day after infec- FIG. 4. Dimerization assays showing the absence of normal dimers in the ⌬⌿ ...the gag initiation codon were transcribed in ...in dimerization buffer, then placed on ice ... See full document

... protection analysis with a riboprobe capable of detecting both wild-type and mutant RNAs ...the gag gene from pNLNh and is capable of distinguishing the wild-type and each mutant ...was ... See full document

... and Gag were subjected to membrane flotation, and individual fractions were analyzed by Western ...blotting. Gag was detected in the top and bottom fractions of the gradient, while control proteins were ... See full document

... of Gag is essential for Gag-Gag multimerization in ...particle assembly do not require myristoylated Gag ...retroviral Gag proteins are stimulated to multimerize through ... See full document

... HIV-1 Gag-expressing ...that Gag-expressing cells should exhibit enhanced and/or pro- longed EGFR ...either Gag, TSG101, or an empty vector and EGFR-CFP with EGF for 10 min, washed them, and ... See full document

... HIV-1 assembly occurs predominantly on the plasma ...(MDM), assembly and budding take place in an intracellular compartment identified as the multivesicular body (MVB) or late endosome (31, 36, 40, ... See full document

... the human immunodeficiency virus type 1 (HIV-1) genome, termed the c locus, which appears essential for initiation of genomic dimerization, as well as for interactions ... See full document

... after assembly (22); similar suggestions have been made with respect to the RSV system ...virion assembly in these mutants may have interfered with p24 production, resulting in the accumulation of p25 in ... See full document

... reporter virus and VSV-G pseudotyped Renilla firefly reporter virus (in which the firefly reporter gene was replaced by Renilla ...pseudotype induced luciferase activity close to (about 75%) that of ... See full document

... A Phylogenetic tree analysis comparing HIV-1 reverse transcriptase nucleotide sequences obtained by direct sequencing of PBMC from the male, the female, 10 unrelated intravenous-drug abu[r] ... See full document

... that Gag is enveloped in membrane ...of Gag are trapped inside membrane ves- icles during homogenization, because ␤-Gal, a cytosolic pro- tein which forms a tetramer of ⬃440 kDa, fractionates primar- ily in ... See full document

... of virus particles attached at the cell surface, as observed by flow cytometry analysis (data not shown), and led to a contamination of both the vesicular and cytosolic fractions with extracellular ... See full document

... particle assembly might suggest that assembly is driven simply by pro- tein-protein interactions between Gag ...for assembly but that other RNAs from the cell can perform this function in the ... See full document

... DRM-associated Gag/GagPol and Gag/Gag ...for 1 h at 4°C to produce the S100 and P100 ...blots: 1, P100 fraction probed with anti-caveolin-1; 2, P100 fraction probed with ... See full document

... by analysis of the HIV-1 capsid ...in human cells because target cell CypA blocks HIV-1 ...Lv1 virus complex, as revealed by a decrease in restriction on CSA addition in simian ...a ... See full document

... the assembly domains abolish or severely impair particle production, regions that lie outside these domains can be deleted without any effect on particle release or ...for assembly process play critical ... See full document