[PDF] Top 20 Analysis of Human Immunodeficiency Virus Type 1 Gag Ubiquitination

... that Gag domains other than p6 have the potential to be ubiquitinated, we wanted to test whether HIV-1 Gag proteins lacking lysine residues in p6 are still ...Furthermore, ubiquitination of ... See full document

... or Gag ⌬ ...or Gag⌬p6. Deproteinization was performed through the addition of 1 ml of proteinase K (5 mg/ml) to the reactions, which were incubated at 37°C for 30 min, followed by phenol-chloro- form ... See full document

... Envelope association with lipid rafts is eliminated in the absence of the Gag precursor. We first investigated whether HIV-1 envelope required other viral proteins to associate with DRMs. Envelope was ... See full document

... Human immunodeficiency virus type 1 (HIV-1) recombination occurs during reverse transcription when parts of the two copackaged RNAs are used as templates for DNA ...HIV-1 ... See full document

... within Gag that mediate Gag-lipid raft ...in Gag-lipid raft interactions through the use of Gag-GFP fusion constructs that had previously been characterized for I-domain function by particle ... See full document

... full-length gag gene clone (31), was fused to green fluorescent protein in pEGFPN1 (Clontech, Palo Alto, ...(American Type Cul- ture Collection) and HeLa S3 cells (a kind gift from Robert ... See full document

... the human immunodeficiency virus type 1 (HIV-1) Gag-Pol precursor protein results from a ⴚ 1 ribosomal frameshifting ...generates Gag and Gag-Pol in a ... See full document

... of human immunodeficiency virus type 1 (HIV-1) and other lentiviruses is required for efficient replication in primary cells and certain immortalized cell lines in vitro and, in ... See full document

... p6 Gag deletion mutation, two consecutive stop codons were introduced into the N-terminal-coding region of the p6 gene (nucleotide [nt] ...the gag reading frame terminated at codon 449, was referred to as ... See full document

... The gag polyprotein is cleaved into the mature viral proteins by the viral protease at the time of particle budding 71, but it is probably the gag precursor polyprotein and not any of it[r] ... See full document

... Our results complement two prior studies of CTL responses in vaccine recipients who had subsequent HIV-1 infection. Betts et al. examined an individual who received a recombinant canarypox vaccine (vCP205), who ... See full document

... particulate Gag in the form of VLPs as a candidate AIDS ...HIV-1 Gag VLPs in mice is complicated by the failure of HIV-1 Gag to efficiently assemble VLPs in murine ...chimeric ... See full document

... Sequence analysis and phylogenetic programs. Clustal V (18) and the Genet- ics Computer Group program PILEUP were used to align the deduced gag amino acid sequences. PRETTY was used to generate a consensus ... See full document

... into Gag VLPs in yeast when Gag was supplied in ...the Gag VLPs encased in yeast cell membrane induced maturation and cross-presentation of dendritic cells ...produced Gag VLPs packaging the ... See full document

... HIV-1 Gag to the plasma membrane ...facilitate Gag-membrane binding [26], we decided to examine whether the E2 expression has an effect on ARF proteins and mRNA ...blotting analysis revealed ... See full document

... CyPB- Gag interaction is much less sensitive to disruption by CsA than is the CyPA-Gag interaction ...blot analysis of protein complexes obtained from a binding experiment in which GST-DCyPB ... See full document

... examine Gag membrane ...wild type, only the membrane flotation assay can separate membrane-bound ma- terial from non-membrane-bound protein complexes such as the recently described detergent-resistant ... See full document

... of gag transcripts are further decreased by the presence of a relatively high AU content and destabilizing AUUUA motifs (inhibitory se- quences ...HIV-1 gag (29), but these modifications reduced the ... See full document

... to Gag antigens indicates a good prognosis, presents a paradox: anti-Gag antibodies have few documented antiviral activities, while the envelope glycoproteins are the targets for essentially all ... See full document

... (CD4 1 HeLa cells) were maintained in Dulbecco’s modified Eagle’s me- dium supplemented with 10% heat-inactivated fetal calf serum, penicillin, and ...plates 1:4 onto 10-cm-diam- eter plates 24 h before ... See full document